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Optical coherence tomography (OCT) is being increasingly adopted as a label-free and non-invasive technique for biomedical applications such as cancer and ocular disease diagnosis. Diagnostic information for these tissues is manifest in…

This work compares two tomographic imaging technologies, time-domain full-field optical coherence tomography (FFOCT) working in reflection and optical transmission tomography (OTT), using a new optical setup that combines both. We show…

Optoacoustic tomography (OAT), also known as photoacoustic tomography, is a rapidly emerging hybrid imaging technique that possesses great potential for a wide range of biomedical imaging applications. In OAT, a laser is employed to…

Medical Physics · Physics 2016-12-21 Yang Lou , Kun Wang , Alexander. A. Oraevsky , Mark. A. Anastasio

Transmission optical coherence tomography (OCT) enables analysis of biological specimens in vitro through detection of forward scattered light. Up to now, transmission OCT was considered as a technique that cannot directly retrieve…

Optics · Physics 2024-05-24 Wojciech Krauze , Martyna Mazur , Arkadiusz Kuś

Various imaging techniques have significantly enhanced our ability to visualize objects embedded within complex media such as biological tissues, fog, atmosphere, or various turbid media. Optical imaging, in particular, offers multiple…

Optics · Physics 2025-05-05 Mingwei He , Sujit K. Sahoo , Chengyuan Xiao , Cuong Dang

Three-dimensional molecular imaging of living cells is essential for unraveling cellular metabolism and response to therapies. However, existing volumetric methods, including fluorescence microscopy and quantitative phase imaging, either…

While optical coherence tomography (OCT) provides a resolution down to 1 micrometer it has difficulties to visualize cellular structures due to a lack of scattering contrast. By evaluating signal fluctuations, a significant contrast…

Dynamic optical coherence tomography (DOCT) statistically analyzes fluctuations in time-sequential OCT signals, enabling label-free and three-dimensional visualization of intratissue and intracellular activities. Current DOCT methods, such…

Optical coherence tomography (OCT) is a powerful technology for rapid volumetric imaging in biomedicine. The bright field imaging approach of conventional OCT systems is based on the detection of directly backscattered light, thereby…

Optical coherence tomography (OCT) and confocal microscopy are pivotal in retinal imaging, offering distinct advantages and limitations. In vivo OCT offers rapid, non-invasive imaging but can suffer from clarity issues and motion artifacts,…

Image and Video Processing · Electrical Eng. & Systems 2024-12-18 Xin Tian , Nantheera Anantrasirichai , Lindsay Nicholson , Alin Achim

Measurement of local temperature using label-free optical methods has gained importance as a pivotal tool in both fundamental and applied research. Yet, most of these approaches are limited to steady-state measurements of planar heat…

Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, have limited ability to provide…

Biological Physics · Physics 2016-11-08 Kyoohyun Kim , SeoEun Lee , Jonghee Yoon , JiHan Heo , Chulhee Choi , YongKeun Park

Imaging airway tissue, optical coherence tomography (OCT) provides cross-sectional images of tissue structures, shows cilia movement and mucus secretion, but does not provide sufficient contrast to differentiate individual cells. By using…

Optical coherence tomography (OCT) is a non-invasive 3D modality widely used in ophthalmology for imaging the retina. Achieving automated, anatomically coherent retinal layer segmentation on OCT is important for the detection and monitoring…

Image and Video Processing · Electrical Eng. & Systems 2022-10-26 Botond Fazekas , Guilherme Aresta , Dmitrii Lachinov , Sophie Riedl , Julia Mai , Ursula Schmidt-Erfurth , Hrvoje Bogunovic

We present a novel illumination control technique for optical diffraction tomography (ODT). Various spatial frequencies of beam illumination were controlled by displaying time-averaged sinusoidal patterns using a digital micromirror device…

Biological Physics · Physics 2017-04-05 Kyeoreh Lee , Kyoohyun Kim , Geon Kim , Seungwoo Shin , YongKeun Park

Recent advancements in the ability to construct three-dimensional (3D) tissues and organoids from stem cells and biomaterials have not only opened abundant new research avenues in disease modeling and regenerative medicine but also have…

Tissues and Organs · Quantitative Biology 2017-07-27 Richard J. McMurtrey

The evaluation and monitoring of cells health in the human retina is crucial and follow time course of retinal diseases, detect lesions before irreversible visual loss and to evaluate treatment effects. Towards this goal, a major challenge…

We developed a new endogenous approach to reveal subcellular metabolic contrast in fresh ex vivo tissues taking advantage of the time dependence of the full field optical coherence tomography interferometric signals. This method reveals…

Medical Physics · Physics 2016-01-07 Clement Apelian , Fabrice Harms , Olivier Thouvenin , A. Claude Boccara

Optical tomographic cross-sectional images of biological samples were made possible by interferometric imaging techniques such as Optical Coherence Tomography (OCT). Owing to its unprecedented view of the sample, OCT has become a gold…

Medical Physics · Physics 2020-07-13 Pedro Mecê , Elena Gofas-Salas , Michel Paques , Kate Grieve , Serge Meimon

Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…

Computer Vision and Pattern Recognition · Computer Science 2023-09-22 Md Shazid Islam , Arindam Dutta , Calvin-Khang Ta , Kevin Rodriguez , Christian Michael , Mark Alber , G. Venugopala Reddy , Amit K. Roy-Chowdhury