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It is widely believed that the folding of the chromosome in the nucleus has a major effect on genetic expression. For example co-regulated genes in several species have been shown to colocalize in space despite being far away on the DNA…

Quantitative Methods · Quantitative Biology 2020-04-29 Guillaume Le Treut , François Képès , Henri Orland

Increasing evidence suggests that chromosome folding and genetic expression are intimately connected. For example, the co-expression of a large number of genes can benefit from their spatial co-localization in the cellular space.…

Soft Condensed Matter · Physics 2018-03-13 Guillaume Le Treut

Three-dimensional (3D) chromatin structure is closely related to genome function, in particular transcription. However, the folding path of the chromatin fiber in the interphase nucleus is unknown. Here, we systematically measured the 3D…

The 3D folding of a mammalian gene can be studied by a polymer model, where the chromatin fibre is represented by a semiflexible polymer which interacts with multivalent proteins, representing complexes of DNA-binding transcription factors…

Biological Physics · Physics 2023-12-20 Andrea Bonato , Dom Corbett , Sergey Kitaev , Davide Marenduzzo , Alexander Morozov , Enzo Orlandini

The probability of two loci, separated by a certain genome length, being in contact can be inferred using the Chromosome Conformation Capture (3C) method and related Hi-C experiments. How to go from the contact map, a matrix listing the…

Soft Condensed Matter · Physics 2021-03-24 Guang Shi , D. Thirumalai

Experimental approaches have been applied to address questions in understanding three-dimensional chromatin organisation and function. As datasets increase in size and complexity, it becomes a challenge to reach a mechanistic interpretation…

Biological Physics · Physics 2020-10-14 Chris A. Brackley , Davide Marenduzzo , Nick Gilbert

Several experiments show that the three dimensional (3D) organization of chromosomes affects genetic processes such as transcription and gene regulation. To better understand this connection, researchers developed the Hi-C method that is…

Genomics · Quantitative Biology 2019-05-03 Sang Hoon Lee , Yeonghoon Kim , Sungmin Lee , Xavier Durang , Per Stenberg , Jae-Hyung Jeon , Ludvig Lizana

Chromatin and associated proteins constitute the highly folded structure of chromosomes. We consider a self-avoiding polymer model of the chromatin, segments of which may get cross-linked via protein binders that repel each other. The…

Soft Condensed Matter · Physics 2019-06-13 Amit Kumar , Debasish Chaudhuri

The three dimensional structure of DNA in the nucleus (chromatin) plays an important role in many cellular processes. Recent experimental advances have led to high-throughput methods of capturing information about chromatin conformation on…

Genomics · Quantitative Biology 2015-11-05 Kevin Emmett , Benjamin Schweinhart , Raul Rabadan

We propose a polymer model for the dynamics of chromatin replication in three dimensional space. Our simulations indicate that both immobile and tracking replisomes may self-assemble during the process, reconciling previous apparently…

Biological Physics · Physics 2023-12-19 G. Forte , S. Buonomo , P. R. Cook , N. Gilbert , D. Marenduzzo , E. Orlandini

We discuss a polymer model for the 3D organization of human chromosomes. A chromosome is represented by a string of beads, with each bead being "colored" according to 1D bioinformatic data (e.g., chromatin state, histone modification, GC…

Biological Physics · Physics 2020-10-15 C. A. Brackley , D. Michieletto , F. Mouvet , J. Johnson , S. Kelly , P. R. Cook , D. Marenduzzo

How long threadlike eukaryotic chromosomes fit tidily in the small volume of the nucleus without significant entanglement is just beginning to be understood, thanks to major advances in experimental techniques. Several polymer models, which…

Soft Condensed Matter · Physics 2024-10-03 D. Thirumalai , Guang Shi , Sucheol Shin , Changbong Hyeon

Chromosome organisation is increasingly recognised as an essential component of genome regulation, cell fate and cell health. Within the realm of transposable elements (TEs) however, the spatial information of how genomes are folded is…

Biological Physics · Physics 2019-11-01 Alexandros Bousios , Hans-Wilhelm Nuetzmann , Dorothy Buck , Davide Michieletto

The development of multicellular organisms relies on the precise coordination of molecular events across multiple spatial and temporal scales. Understanding how information flows from molecular interactions to cellular processes and tissue…

Biological Physics · Physics 2025-01-29 Andrés H. Cardona , Márcia Mesquita Peixoto , Tohn Borjigin , Thomas Gregor

To better understand DNA's 3D folding in cell nuclei, researchers developed chromosome capture methods such as Hi-C that measure the contact frequencies between all DNA segment pairs across the genome. As Hi-C data sets often are massive,…

Biological Physics · Physics 2023-11-14 Anton Holmgren , Dolores Bernenko , Ludvig Lizana

Three-dimensional interphase organization of metazoan genomes has been linked to cellular identity. However, the principles governing 3D interphase genome architecture and its faithful transmission through disruptive events of cell-cycle,…

Subcellular Processes · Quantitative Biology 2016-04-12 Davide Michieletto , Davide Marenduzzo , Ajazul H. Wani

Polymer models are used to describe chromatin, which can be folded at different spatial scales by binding molecules. By folding, chromatin generates loops of various sizes. We present here a randomly cross-linked (RCL) polymer model, where…

Soft Condensed Matter · Physics 2017-05-12 Ofir Shukron , David Holcman

Three-Dimensional (3D) chromatin interactions, such as enhancer-promoter interactions (EPIs), loops, Topologically Associating Domains (TADs), and A/B compartments play critical roles in a wide range of cellular processes by regulating gene…

Genomics · Quantitative Biology 2024-03-07 Brydon P. G. Wall , My Nguyen , J. Chuck Harrell , Mikhail G. Dozmorov

Understanding the three-dimensional (3D) structure of the genome is essential for elucidating vital biological processes and their links to human disease. To determine how the genome folds within the nucleus, chromosome conformation capture…

The statistical properties of intra-chromosome maps obtained by a genome-wide chromosome conformation capture method (Hi-C) are described in the framework of the hierarchical crumpling model of heteropolymer chain with quenched disorder in…

Genomics · Quantitative Biology 2013-12-02 L. Nazarov , M. V. Tamm , S. K. Nechaev , V. A. Avetisov
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