Related papers: Super-resolution deep imaging with hollow Bessel b…
Stimulated Emission Depletion (STED) microscopy has emerged as a powerful technique providing visualization of biological structures at the molecular level in living samples. In this technique, the diffraction limit is broken by selectively…
With recent developments in microscopy, such as stimulated emission depletion (STED) microscopy, far-field imaging at resolutions better than the diffraction limit is now a commercially available technique. Here, we show that, in the…
Stimulated emission depletion, or STED microscopy is a well-established super-resolution technique, but is ultimately limited by the chosen flourophore. Here we demonstrate STED microscopy with color centers in nanoscale flakes of hexagonal…
Magnetic particle imaging (MPI) is an in-vivo imaging method to detect magnetic nanoparticles for blood vessel imaging and molecular target imaging. Compared with conventional molecular imaging devices (such as nuclear medicine imaging PET…
We demonstrate stimulated emission depletion (STED) microscopy with 20 nm gold nanospheres coated by fluorescent silica. Compared with previous demonstrations of STED with a hybrid plasmonic fluorescent label, the current implementation…
Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-scanning. This constitutes…
Stimulated Emission Depletion Microscopy (STED) can achieve a spatial resolution as high as several nanometers. As a point scanning imaging method, it requires 3D scanning to complete the imaging of 3D samples. The time-consuming 3D…
We propose a novel stimulated emission depletion (STED) microscopy based on array detection and photon reassignment. By replacing the single-point detector in traditional STED with a detector array and utilizing the photon reassignment…
Accurate three-dimensional (3D) imaging requires an isotropic point spread function (PSF). However, the inherent missing aperture of a single objective lens results in an elongated, cigar-like PSF, which has rendered isotropic resolution in…
Plasmonic nanoparticles influence the absorption and emission processes of nearby emitters due to local enhancements of the illuminating radiation and the photonic density of states. Here, we use the plasmon resonance of metal nanoparticles…
This thesis centres on the development of multidimensional fluorescence imaging tools, with a particular emphasis on fluorescence lifetime imaging (FLIM) microscopy for application to biological research. The key aspects of this thesis are…
Far-field optical microscopy using focused light is an important tool in a number of scientific disciplines including chemical, (bio)physical and biomedical research, particularly with respect to the study of living cells and organisms.…
Photobleaching is a major limitation of superresolution Stimulated Depletion Emission (STED) microscopy. Fast scanning has long been considered an effective means to reduce photobleaching in fluorescence microscopy, but a careful…
Fluorescence depletion microscopy techniques such as STED and RESOLFT require optical fields with a well-defined and spatially confined central intensity minimum to achieve sub-diffraction lateral resolution. Here, we present the design and…
The China Space Station Telescope (CSST) will conduct wide-field multiband photometric imaging and slitless spectroscopic surveys, advancing cosmology and galaxy evolution studies. Achieving CSST's cosmological goals requires precise…
In the context of simulating precision laser interferometers, we compare via several examples two wavefront decomposition methods: the Mode Expansion Method (MEM) and the Gaussian beam decomposition (GBD) for their precision and…
Aberration-corrected scanning transmission electron microscopes (STEM) provide sub-angstrom lateral resolution; however, the large convergence angle greatly reduces the depth of field. For microscopes with a small depth of field,…
To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…
Stimulated emission depletion (STED) can achieve optical super-resolution, with the optical diffraction limit broken by the suppression on the periphery of the fluorescent focal spot. Previously, it is generally experimentally accepted that…
Accurate medical image segmentation is fundamental to precision medicine, yet robust delineation remains challenging under heterogeneous appearances, ambiguous boundaries, and large anatomical variability. Similar intensity and texture…