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We propose a speed-up method for the in-focus plane detection in digital holographic microscopy that can be applied to a broad class of autofocusing algorithms that involve repetitive propagation of an object wave to various axial locations…

Quantitative Methods · Quantitative Biology 2023-05-19 Julianna Winnik , Damian Suski , Piotr Zdańkowski , Luiza Stanaszek , Vicente Micó , Maciej Trusiak

Current biological and medical research is aimed at obtaining a detailed spatiotemporal map of a live cell's interior to describe and predict cell's physiological state. We present here an algorithm for complete 3-D modelling of cellular…

Quantitative Methods · Quantitative Biology 2017-04-03 Renata Rychtarikova , Tomas Nahlik , Kevin Shi , Daria Malakhova , Petr Machacek , Rebecca Smaha , Jan Urban , Dalibor Stys

X-ray fluorescence holography (XFH) is a method for obtaining diffraction-limited images of the local atomic structure around a given type of emitter. The reconstructed wave-field represents a distorted image of the scatterer electron…

Condensed Matter · Physics 2009-09-29 S. Marchesini , C. S. Fadley , F. J. Garcia de Abajo

Automated label-free quantitative imaging of biological samples can greatly benefit high throughput diseases diagnosis. Digital holographic microscopy (DHM) is a powerful quantitative label-free imaging tool that retrieves structural…

A method is proposed for high-resolution, three-dimensional reconstruction of internal structure of objects from planar transmission images. The described approach can be used with any form of radiation or matter waves, in principle,…

Optics · Physics 2022-08-24 T. E. Gureyev , H. M. Quiney , L. J. Allen

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

We present a dual-stage neural network architecture for analyzing fine shape details from microscopy recordings in 3D. The system, tested on red blood cells, uses training data from both healthy donors and patients with a congenital blood…

Quantitative Methods · Quantitative Biology 2020-05-29 G. Simionato , K. Hinkelmann , R. Chachanidze , P. Bianchi , E. Fermo , R. van Wijk , M. Leonetti , C. Wagner , L. Kaestner , S. Quint

Digital holography is an interference-based imaging technique capable of recording both the amplitude and phase of an electromagnetic field. It can be obtained at the laser illumination wavelength, but also with second-harmonic generation,…

We introduce a practical digital holographic method capable of imaging through a diffusive or scattering medium. The method relies on statistical averaging from a rotating ground glass diffuser to negate the adverse effects caused by…

Optical tomography has emerged as a non-invasive imaging method, providing three-dimensional insights into subcellular structures and thereby enabling a deeper understanding of cellular functions, interactions, and processes. Conventional…

Medical Physics · Physics 2023-12-14 Jiawei Sun , Bin Yang , Nektarios Koukourakis , Jochen Guck , Juergen W. Czarske

In-line holographic video microscopy records a wealth of information about the microscopic structure and dynamics of colloidal materials. Powerful analytical techniques are available to retrieve that information when the colloidal particles…

Soft Condensed Matter · Physics 2024-03-19 Jatin Abacousnac , Jasna Brujic , David G. Grier

Off-axis X-ray holography is a lensless imaging technique that allows unambiguous retrieval of an object's exit-wave function with high fidelity. It has been used with great success to study nanoscale phenomena and spatio-temporal dynamics…

We use digital holographic microscopy and Mie scattering theory to simultaneously characterize and track individual colloidal particles. Each holographic snapshot provides enough information to measure a colloidal sphere's radius and…

We experimentally test a recently proposed holographic method for imaging coherent light scatterers which are distributed over a 2-dimensional grid. In our setup the scatterers consist of a back-illuminated, opaque mask with submicron-sized…

Holotomography, a three-dimensional quantitative phase imaging technique, presents an innovative, non-invasive approach to studying biological samples by exploiting the refractive index as an intrinsic imaging contrast. Despite offering…

Optics · Physics 2023-08-04 Herve Hugonnet , HyunJun Han , Weisun Park , YongKeun Park

Digital holography has applications in bio-imaging because it can simultaneously obtain the amplitude and phase information of a microscopic sample in a single shot, thus facilitating non-contact, noninvasive observation of the 3D shape of…

Graphics · Computer Science 2024-06-14 Yuki Nagahama

A new scheme based on (i) upcoming brilliant X-ray Free Electron Laser (FEL) sources, (ii) novel energy and angular dispersive, large-area electron imagers and (iii) the well-known photoelectron holography is elaborated that provides…

Atomic Physics · Physics 2015-05-14 F. Krasniqi , B. Najjari , L. Strüder , D. Rolles , A. Voitkiv , J. Ullrich

In radio astronomy, holography is a commonly used technique to create an image of the electric field distribution in the aperture of a dish antenna. The image is used to detect imperfections in the reflector surface. Similarly, holography…

Instrumentation and Methods for Astrophysics · Physics 2021-06-09 U. Kiefner , R. B. Wayth , D. B. Davidson , M. Sokolowski

Holography relies on the interference between a known reference and a signal of interest to reconstruct both the amplitude and phase of that signal. Commonly performed with photons and electrons, it finds numerous applications in imaging,…

Wireless data transmission systems such as WiFi or Bluetooth emit coherent light - electromagnetic waves with precisely known amplitude and phase. Propagating in space, this radiation forms a hologram - a two-dimensional wavefront encoding…

Classical Physics · Physics 2017-05-10 Philipp Holl , Friedemann Reinhard