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Optical imaging plays a critical role in advancing our understanding of three dimensional dynamics of biological systems. Coherent imaging (CI) methods exploit spatial phase information, encoded through propagation of coherent signal light…

Optics · Physics 2015-07-24 Jeffrey J. Field , David G. Winters , Randy A. Bartels

This paper describes a novel method for partitioning image into meaningful segments. The proposed method employs watershed transform, a well-known image segmentation technique. Along with that, it uses various auxiliary schemes such as…

Computer Vision and Pattern Recognition · Computer Science 2013-03-21 Ankit R. Chadha , Neha S. Satam

In coherent X-ray diffraction microscopy the diffraction pattern generated by a sample illuminated with coherent x-rays is recorded, and a computer algorithm recovers the unmeasured phases to synthesize an image. By avoiding the use of a…

The ability to resolve and quantify features at submicrometer scales from a single-shot image is crucial for real-time uncovering intricate structures in unlabeled biological samples and analyzing them at the subcellular level. We introduce…

Optics · Physics 2025-01-22 Jaromír Běhal , Miroslav Ježek

We demonstrate a novel imaging approach and associated reconstruction algorithm for far-field coherent diffractive imaging, based on the measurement of a pair of laterally sheared diffraction patterns. The differential phase profile…

Optics · Physics 2018-05-23 G. S. M. Jansen , A. C. C. de Beurs , X. Liu , K. S. E. Eikema , S. Witte

Phase retrieval is a nonlinear inverse problem that arises in a wide range of imaging modalities, from electron microscopy to Fourier ptychography. In particular, the reconstruction is facilitated when the sensing matrix is i.i.d. random,…

On 3D imaging, light field cameras typically are of single shot, and however, they heavily suffer from low spatial resolution and depth accuracy. In this paper, by employing an optical projector to project a group of single high-frequency…

Image and Video Processing · Electrical Eng. & Systems 2024-04-11 Geyou Zhang , Ce Zhu , Kai Liu , Yipeng Liu

In this paper, we develop a novel phase retrieval approach to reconstruct x-ray differential phase shift induced by an object. A primary advantage of our approach is a higher-order accuracy over that with the conventional linear…

Medical Physics · Physics 2010-03-18 Wenxiang Cong , Ge Wang

We introduce OCULAR, an innovative hardware and software solution for three-dimensional dynamic image analysis of fine particles. Current state-of-the art instruments for dynamic image analysis are largely limited to two-dimensional…

Computer Vision and Pattern Recognition · Computer Science 2024-12-10 Sadegh Nadimi , Vasileios Angelidakis , Sadaf Maramizonouz , Chao Zhang

A semi-analytical computational algorithm to model the wavefield generated by paraxial diffraction of a class of Laguerre-Gauss beams by sharp-edge elliptic apertures is here developed. Thanks to such a powerful computational tool, some…

Optics · Physics 2024-02-05 Riccardo Borghi

Phase reconstruction is important in transmission electron microscopy for structural studies. We describe electron Fourier ptychography and its application to phase reconstruction of both radiation-resistant and beam-sensitive materials. We…

Prostate specific membrane antigen (PSMA) positron emission tomography/computed tomography (PET/CT) imaging provides a tremendously exciting frontier in visualization of prostate cancer (PCa) metastatic lesions. However, accurate…

Medical Physics · Physics 2024-12-05 Amirhosein Toosi , Sara Harsini , François Bénard , Carlos Uribe , Arman Rahmim

We present a Machine Learning-based method for tomographic reconstruction of dense layered objects, with range of projection angles limited to $\pm $10$^\circ$. Whereas previous approaches to phase tomography generally require two steps,…

Image and Video Processing · Electrical Eng. & Systems 2020-01-08 Alexandre Goy , Girish Rughoobur , Shuai Li , Kwabena Arthur , Akintunde I. Akinwande , George Barbastathis

Detection of phase variations across optically transparent samples is often a difficult task. We propose and demonstrate a compact, lightweight and low cost quantitative phase contrast imager. Light diffracted from a pinhole is incident on…

We present a novel approach to single-shot characterization of the spectral phase of broadband laser pulses. Our method is inexpensive, insensitive to alignment and combines the simplicity and robustness of the dispersion scan technique,…

Formation of a bright-field microscopic image of a transparent phase object is described in terms of elementary geometrical optics. Our approach is based on the premise that image replicates the intensity distribution (real or virtual) at…

Optics · Physics 2018-02-14 A. K. Khitrin , M. A. Model

Based on point spread function (PSF) engineering and astigmatism due to a pair of cylindrical lenses, a novel compressed imaging mechanism is proposed to achieve single-shot incoherent 3D imaging. The speckle-like PSF of the imaging system…

Optics · Physics 2021-05-24 Qiong Gao , Weidong Qu , Ming Shao , Wei Liu , Xiangzheng Cheng

Imaging 3D nano-structures at very high resolution is crucial in a variety of scientific fields. However, due to fundamental limitations of light propagation we can only measure the object indirectly via 2D intensity measurements of the 3D…

Image and Video Processing · Electrical Eng. & Systems 2020-02-28 Zalan Fabian , Justin Haldar , Richard Leahy , Mahdi Soltanolkotabi

We demonstrate numerical refocusing in coherent confocal laser scanning microscopy based on synthetic optical holography. This physics-based approach implements a computational propagation on the complex signal recovered in synthetic…

The optical resolution of a digital camera is one of its most crucial parameters with broad relevance for consumer electronics, surveillance systems, remote sensing, or medical imaging. However, resolution is physically limited by the…

Computer Vision and Pattern Recognition · Computer Science 2018-12-27 Thomas Köhler