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Optical nanoscopy is crucial in life and materials sciences, revealing subtle cellular processes and nanomaterial properties. Scattering-type Scanning Near-field Optical Microscopy (s-SNOM) provides nanoscale resolution, relying on the…

High-speed atomic force microscopy (HS-AFM) is an indispensable technique in the biological field owing to its excellent imaging capability for the real-time observation of biomolecules with high spatial resolution. Furthermore, recent…

Artificial intelligence (AI) and machine learning have promised to revolutionize the way we live and work, and one of particularly promising areas for AI is image analysis. Nevertheless, many current AI applications focus on post-processing…

Materials Science · Physics 2020-07-31 Boyuan Huang , Zhenghao Li , Jiangyu Li

X-ray ptychography has revolutionised nanoscale phase contrast imaging at large-scale synchrotron sources in recent years. We present here the first successful demonstration of the technique in a small-scale laboratory setting. We conducted…

Dynamic-mode atomic force microscopy (AFM) in liquid remains complicated due to the strong viscous damping of the cantilever resonance. Here we show that a high-quality resonance (Q>20) can be achieved in aqueous solution by attaching a…

Instrumentation and Detectors · Physics 2015-05-14 Sebastian Hoof , Nitya Nand Gosvami , Bart W. Hoogenboom

Nowadays powerful X-ray sources like synchrotrons and free-electron lasers are considered as ultimate tools for probing microscopic properties in materials. However, the correct interpretation of such experiments requires a good…

Materials Science · Physics 2018-02-28 B. Ruta , F. Zontone , Y. Chushkin , G. Baldi , G. Pintori , G. Monaco , B. Rufflé , W. Kob

We report on the use of 4.7-$\mu$m-diameter, optically levitated, charged microspheres to image the three-dimensional force field produced by charge distributions on an Au-coated, microfabricated Si beam in vacuum. An upward-propagating,…

Instrumentation and Detectors · Physics 2019-02-13 Charles P. Blakemore , Alexander D. Rider , Sandip Roy , Qidong Wang , Akio Kawasaki , Giorgio Gratta

We propose an imaging system and methodology for mapping soft-tissue samples in three dimensions, with micron-scale and isotropic spatial resolution, with low-concentrations as well as in the absence of heavy metal staining. We used hard…

Instrumentation and Detectors · Physics 2023-12-21 Michela Esposito , Nicole Schieber , Alessandro Olivo , Yannick Schwab , Marco Endrizzi

Three-dimensional atomic force microscopy (3D-AFM) has been a powerful tool to probe the atomic-scale structure of solid-liquid interfaces. As a nanoprobe moves along the 3D volume of interfacial liquid, the probe-sample interaction force…

A device based on a three-block Fresnel zone plate interferometer is proposed for hard X-ray phase-contrast imaging. The device combines a low requirement for the coherence of the initial radiation (the interferometer operates in the…

Instrumentation and Detectors · Physics 2021-04-22 L. A. Haroutunyan

Atomic force microscopy (AFM) is a mechanical profiling technique that allows to image surfaces with atomic resolution. Recent progress in reducing the noise of this technique has led to a resolution level where previously undetectable…

Materials Science · Physics 2015-06-24 F. J. Giessibl , H. Bielefeldt , S. Hembacher , J. Mannhart

In a scanning transmission electron microscope (STEM), producing a high-resolution image generally requires an electron beam focused to the smallest point possible. However, the magnetic lenses used to focus the beam are unavoidably…

Microscopy is an essential tool in scientific research, enabling the visualization of structures at micro- and nanoscale resolutions. However, the field of microscopy often encounters limitations in field-of-view (FOV), restricting the…

Image and Video Processing · Electrical Eng. & Systems 2025-03-17 Huanhuan Zhao , Ruben Millan-Solsona , Marti Checa , Spenser R. Brown , Jennifer L. Morrell-Falvey , Liam Collins , Arpan Biswas

Atomic force microscopes (AFMs) are ubiquitous in research laboratories and have recently been priced for use in teaching laboratories. Here we review several AFM platforms (Dimension 3000 by Digital Instruments, EasyScan2 by Nanosurf,…

Photoacoustic microscopy is becoming an important tool for the biomedical research. It has been widely used in biological researches, such as structural imaging of vasculature, brain structural and functional imaging, and tumor detection.…

Image and Video Processing · Electrical Eng. & Systems 2020-09-09 Xianlin Song , Jianshuang Wei , Lingfang Song

We present a multi-aperture analyser set-up for performing X-ray phase contrast imaging in planar and three-dimensional modalities. A multi-slice representation of the sample is used to establish a quantitative relation between projection…

Instrumentation and Detectors · Physics 2017-06-21 M. Endrizzi , F. A. Vittoria , L. Rigon , D. Dreossi , A. Olivo

Scanning X-ray nanodiffraction microscopy is a powerful technique for spatially resolving nanoscale structural morphologies by diffraction contrast. One of the critical challenges in experimental nanodiffraction data analysis is posed by…

Applied Physics · Physics 2024-06-26 Aileen Luo , Tao Zhou , Martin V. Holt , Andrej Singer , Mathew J. Cherukara

Atomic Force Microscopy - Infrared (AFM-IR) spectroscopy allows spectroscopic studies in the mid-infrared spectral region with a spatial resolution better than 50 nm. We show that the high spatial resolution can be used to perform…

Quantitative Methods · Quantitative Biology 2017-04-06 Luca Quaroni , Katarzyna Pogoda , Joanna-Wiltowska Zuber , Wojciech Kwiatek

The description of hydrodynamic interactions between a particle and the surrounding liquid, down to the nanometer scale, is of primary importance since confined liquids are ubiquitous in many natural and technological situations. In this…