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Super-resolution imaging techniques have largely improved our capabilities to visualize nanometric structures in biological systems. Their application further enables one to potentially quantitate relevant parameters to determine the…

Biological Physics · Physics 2019-12-18 Tina Kosǔta , Marta Cullell-Dalmau , Francesca Cella Zanacchi , Carlo Manzo

Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…

Event-based image sensors provide microsecond temporal resolution but lack spectral discrimination, whereas diffractive spectral imagers encode wavelength information at conventional frame rates. We introduce a fluorescence microscopy…

Optics · Physics 2026-03-20 Richard G. Baird , Boyden Myers , Erik M. Jorgensen , Rajesh Menon

One of the main characteristics of optical imaging systems is the spatial resolution, which is restricted by the diffraction limit to approximately half the wavelength of the incident light. Along with the recently developed classical…

High-resolution fMRI provides a window into the brain's mesoscale organization. Yet, higher spatial resolution increases scan times, to compensate for the low signal and contrast-to-noise ratio. This work introduces a deep learning-based 3D…

Image and Video Processing · Electrical Eng. & Systems 2024-03-20 Hongwei Bran Li , Matthew S. Rosen , Shahin Nasr , Juan Eugenio Iglesias

Single Molecule Localization Microscopy (SMLM) enables the acquisition of high-resolution images by alternating between activation of a sparse subset of fluorescent molecules present in a sample and localization. In this work, the…

Image and Video Processing · Electrical Eng. & Systems 2018-12-17 Arne Bechensteen , Laure Blanc-Féraud , Gilles Aubert

Breaking the diffraction limit in microscopy by utilizing quantum properties of light has been the goal of intense research in the recent years. We propose a quantum superresolution technique based on non-classical emission statistics of…

Optics · Physics 2013-05-30 Osip Schwartz , Dan Oron

In single-molecule super-resolution microscopy, engineered point-spread functions (PSFs) are designed to efficiently encode new molecular properties, such as 3D orientation, into complex spatial features captured by a camera. To fully…

Image and Video Processing · Electrical Eng. & Systems 2019-07-16 Hesam Mazidi , Eshan S. King , Oumeng Zhang , Arye Nehorai , Matthew D. Lew

Single molecule applications, saturated pattern excitation microscopy, or stimulated emission depletion (STED) microscopy demand for bright and highly stable fluorescent dyes1,2. Despite of intensive research the choice of fluorphores is…

Mesoscale and Nanoscale Physics · Physics 2011-09-01 Christian Grunwald , Katrin Schulze , Gregory Giannone , Laurent Cognet , Brahim Lounis , Daniel Choquet , Robert Tampé

The integration of entangled photon emitters in nanophotonic structures designed for the broadband enhancement of photon extraction is a major challenge for quantum information technologies. We study the potential of quantum dot (QD)…

This work presents a new super-resolution imaging approach by using subwavelength hole resonances. We employ a subwavelength structure in which an array of tiny holes are etched in a metallic slab with the neighboring distance $\ell$ that…

Optics · Physics 2020-10-07 Junshan Lin , Hai Zhang

We consider simultaneously identifying the membership and locations of point sources that are convolved with different low-pass point spread functions, from the observation of their superpositions. This problem arises in three-dimensional…

Information Theory · Computer Science 2015-04-24 Yuanxin Li , Yuejie Chi

We propose to achieve super-resolution in far-field imaging by use of dual-dye-doped nanoparticles. The nanoparticles with a diameter of a few nanometers are co-doped with two types of dye molecules, namely, Cy3 and Cy5, at a controllable…

Optics · Physics 2015-05-13 Jianfang Chen , Ya Cheng

In Ultrasound Localization Microscopy (ULM), achieving high-resolution images relies on the precise localization of contrast agent particles across a series of beamformed frames. However, our study uncovers an enormous potential: The…

Computational Geometry · Computer Science 2024-04-09 Christopher Hahne , Georges Chabouh , Arthur Chavignon , Olivier Couture , Raphael Sznitman

Super-resolution is a machine-learning technique in image processing which generates high-resolution images from low-resolution images. Inspired by this approach, we perform a numerical experiment of quantum machine learning, which takes…

Quantum Physics · Physics 2022-11-09 Rei Sakuma , Yutaro Iiyama , Lento Nagano , Ryu Sawada , Koji Terashi

We propose an unfolded accelerated projected-gradient descent procedure to estimate model and algorithmic parameters for image super-resolution and molecule localization problems in image microscopy. The variational lower-level constraint…

Numerical Analysis · Mathematics 2024-03-27 Silvia Bonettini , Luca Calatroni , Danilo Pezzi , Marco Prato

We propose and demonstrate a photon-efficient optical classifier to overcome the Rayleigh limit in spatial resolution. It utilizes mode-selective sum frequency generation and single-pixel photon detection to resolve closely spaced…

Quantum Physics · Physics 2020-10-21 He Zhang , Santosh Kumar , Yu-Ping Huang

Single molecule localization microscopy is widely used in biological research for measuring the nanostructures of samples smaller than the diffraction limit. This study uses multifocal plane microscopy and addresses the 3D single molecule…

Computer Vision and Pattern Recognition · Computer Science 2020-01-08 Toshimitsu Aritake , Hideitsu Hino , Shigeyuki Namiki , Daisuke Asanuma , Kenzo Hirose , Noboru Murata

Fluorescence lifetime imaging microscopy (FLIM) provides detailed information about molecular interactions and biological processes. A major bottleneck for FLIM is image resolution at high acquisition speeds, due to the engineering and…

Image and Video Processing · Electrical Eng. & Systems 2024-04-23 Valentin Kapitány , Areeba Fatima , Vytautas Zickus , Jamie Whitelaw , Ewan McGhee , Robert Insall , Laura Machesky , Daniele Faccio

We propose a new wide-field imaging method that exploits the Localized Surface Plasmon Resonance phenomenon to produce super-resolution images with an optical microscope equipped with a custom design polarization analyzer module. In this…

Instrumentation and Methods for Astrophysics · Physics 2023-10-09 Andrey Alexandrov , Takashi Asada , Fabio Borbone , Valeri Tioukov , Giovanni De Lellis