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Single molecule Raman spectroscopy provides information on individual molecules with vibrational-level resolution. The unique mechanisms leading to the huge Raman cross-section enhancement necessary for single molecule sensitivity are under…

Materials Science · Physics 2007-05-23 Gilad Haran

We discuss the influence of the superconducting transition in a film on the fluorescence spectrum of a single molecule located nearby. We show that single molecule spectroscopy (SMS) should be an appropriate tool to detect the electric…

Superconductivity · Physics 2007-05-23 M. Faure , B. Lounis , A. I. Buzdin

We study the influence of a scanning nano-electrode on fluorescence excitation spectra of single terrylene molecules embedded in thin p-terphenyl films at cryogenic temperatures. We show that applied voltages less than 10 V can result in…

Chemical Physics · Physics 2010-09-17 Ilja Gerhardt , Gert Wrigge , Vahid Sandoghdar

Another way to evaluate the spectral-correlation properties of thermal fields of solids is suggested. Such a method takes into account detailed structure of the interface transition layer separating one bulk region from those of the vacuum…

Quantum Physics · Physics 2015-06-15 Illarion Dorofeyev

The advent of single molecule optics has had a profound impact in fields ranging from biophysics to material science, photophysics, and quantum optics. However, all existing room-temperature single molecule methods have been based on…

Mesoscale and Nanoscale Physics · Physics 2010-07-22 Michele Celebrano , Philipp Kukura , Alois Renn , Vahid Sandoghdar

We show that structural information can be extracted from single molecule localization microscopy (SMLM) data. More precisely, we reinterpret SMLM data as the measures of a phaseless optical diffraction tomography system for which the…

Signal Processing · Electrical Eng. & Systems 2023-07-19 Thanh-An Pham , Emmanuel Soubies , Ferréol Soulez , Michael Unser

Fluctuation in fluorescence emission of immobilized single molecule is typically ascribed to the chromophore's intrinsic structural conformations and the influence of local environmental factors. Despite extensive research over several…

Chemical Physics · Physics 2024-08-07 Aranyak Sarkar , Vinu Namboodiri , Manoj Kumbhakar

We present a theory for analyzing residence times of single molecules in a fixed detection area of a scanning tunneling microscope (STM). The approach is developed for one-dimensional molecule diffusion and can be extended to two dimensions…

Mesoscale and Nanoscale Physics · Physics 2022-08-23 Christoph Schiel , Philipp Rahe , Philipp Maass

Single molecule spectroscopy aims at unveiling often hidden but potentially very important contributions of single entities to a system's ensemble response. Albeit contributing tremendously to our ever growing understanding of molecular…

Chemical Physics · Physics 2017-12-19 Matz Liebel , Costanza Toninelli , Niek F. van Hulst

Photothermal spectroscopy and microscopy provides a route to measure the spectral and spatial properties of individual nanoscopic absorbers, independent from scattering, extinction, and emission. The approach relies upon use of two light…

Optics · Physics 2021-04-12 Harrison J. Goldwyn , Stephan Link , David J. Masiello

Photothermal microscopy has recently complemented single molecule fluorescence microscopy by the detection of individual nano-objects in absorption. Photothermal techniques gain their superior sensitivity by exploiting a heat induced…

Optics · Physics 2015-05-28 Markus Selmke , Marco Braun , Frank Cichos

We present scanning near-field extinction spectra of single molecules embedded in a solid matrix. By varying the molecule-tip separation, we modify the line shape of the spectra, demonstrating the coherent nature of the interaction between…

Optics · Physics 2009-11-13 I. Gerhardt , G. Wrigge , M. Agio , P. Bushev , G. Zumofen , V. Sandoghdar

Spectral diffusion is a result of random spectral jumps of a narrow line as a result of a fluctuating environment. It is an important issue in spectroscopy, because the observed spectral broadening prevents access to the intrinsic line…

Frequency domain spectroscopy allows an experimenter to establish optical properties of solids in a wide frequency band including the technically challenging 10 THz region, and in other bands enables metrological comparison between…

Optics · Physics 2018-09-21 Steven Chick , Ben Murdin , Guy Matmon , Mira Naftaly

Methods of determining surface diffusion coefficients of molecules from signal fluctuations of a locally fixed probe are revisited and refined. Particular emphasis is put on the influence of the molecule's extent. In addition to the…

Mesoscale and Nanoscale Physics · Physics 2015-06-12 Susanne Hahne , Julian Ikonomov , Moritz Sokolowski , Philipp Maass

A two level model of a single molecule undergoing spectral diffusion dynamics and interacting with a sequence of two short laser pulses is investigated. Analytical solution for the probability of n=0,1,2 photon emission events for the…

Chemical Physics · Physics 2009-11-13 F. Shikerman , E. Barkai

We investigate theoretically how single-molecule spectroscopy techniques can be used to perform fast and high resolution displacement detection and manipulation of nanomechanical oscillators, such as singly clamped carbon nanotubes. We…

Mesoscale and Nanoscale Physics · Physics 2015-10-21 Vadim Puller , Brahim Lounis , Fabio Pistolesi

Single-molecule fluorescence spectroscopy is a powerful method that avoids ensemble averaging, but its temporal resolution is limited by the fluorescence lifetime to nanoseconds at most. At the ensemble level, two-dimensional spectroscopy…

Optics · Physics 2024-10-11 Sanchayeeta Jana , Simon Durst , Markus Lippitz

Current optical detection schemes for single molecules require light absorption, either to produce fluorescence or direct absorption signals. This severely limits the range of molecules that can be detected, because most molecules are…

Chemical Physics · Physics 2012-07-06 Peter Zijlstra , Pedro M. R. Paulo , Michel Orrit

Single molecule localization microscopy is a recently developed superresolution imaging technique to visualize structural properties of single cells. The basic principle consists in chemically attaching fluorescent dyes to the molecules,…

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