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Fluorescence microscopy is a critical tool across various disciplines, from materials science to biomedical research, yet it is limited by the diffraction limit of resolution. Advanced super-resolution techniques such as localization…

In some super-resolution techniques, adjacent points are illuminated at different times. Thereby, their locations and light intensities can be detected even if the images are very blurred due to diffraction. According to conventional…

Image and Video Processing · Electrical Eng. & Systems 2019-12-10 Edward Y. Sheffield

The diffraction of light imposes a fundamental limit on the resolution of light microscopes. This limit can be circumvented by creating and exploiting independent behaviors of the sample at length scales below the diffraction limit. In…

Biological Physics · Physics 2024-06-19 Mohamadreza Fazel , Michael J. Wester

I explain what is, what is not, and what is only sort of superresolution microscopy. I discuss optical resolution, first in terms of diffraction theory, then in terms of linear systems theory, and finally in terms of techniques that use…

Optics · Physics 2015-04-02 John Bechhoefer

In single molecule localisation super-resolution microscopy the need for repeated image capture limits the imaging speed, while the size of fluorescence probes limits the possible theoretical localisation resolution. Here, we demonstrated a…

Superresolution fluorescence microscopy techniques beat the diffraction limit, enabling ultra-high resolution imaging in biological physics and nanoscience. In all cases that have been studied experimentally, the resolution scales inversely…

Optics · Physics 2012-10-10 Alexander Small

The optical resolution of a digital camera is one of its most crucial parameters with broad relevance for consumer electronics, surveillance systems, remote sensing, or medical imaging. However, resolution is physically limited by the…

Computer Vision and Pattern Recognition · Computer Science 2018-12-27 Thomas Köhler

Various techniques have been developed to measure the 2D and 3D positions and 2D and 3D orientations of fluorescent molecules with improved precision over standard epifluorescence microscopes. Due to the challenging signal-to-background…

Optics · Physics 2021-02-02 Oumeng Zhang , Matthew D. Lew

Super-resolution reconstruction techniques entail the utilization of software algorithms to transform one or more sets of low-resolution images captured from the same scene into high-resolution images. In recent years, considerable…

Computer Vision and Pattern Recognition · Computer Science 2024-08-02 Hao Yan , Zixiang Wang , Zhengjia Xu , Zhuoyue Wang , Zhizhong Wu , Ranran Lyu

Super-resolution microscopy overcomes the diffraction limit of conventional light microscopy in spatial resolution. By providing novel spatial or spatio-temporal information on biological processes at nanometer resolution with molecular…

Biological Physics · Physics 2021-11-10 Tianjie Yang , Yaoru Luo , Wei Ji , Ge Yang

Accurately classifying white blood cells from microscopic images is essential to identify several illnesses and conditions in medical diagnostics. Many deep learning technologies are being employed to quickly and automatically classify…

Image and Video Processing · Electrical Eng. & Systems 2025-08-07 Tatwadarshi P. Nagarhalli , Shruti S. Pawar , Soham A. Dahanukar , Uday Aswalekar , Ashwini M. Save , Sanket D. Patil

Image super-resolution (SR) is a field in computer vision that focuses on reconstructing high-resolution images from the respective low-resolution image. However, super-resolution is a well-known ill-posed problem as most methods rely on…

Computer Vision and Pattern Recognition · Computer Science 2022-10-25 Athiya Deviyani , Efe Sinan Hoplamaz , Alan Savio Paul

Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Hallucinating high frequency image details in single image super-resolution is a challenging task. Traditional super-resolution methods tend to produce oversmoothed output images due to the ambiguity in mapping between low and high…

Computer Vision and Pattern Recognition · Computer Science 2017-01-27 Libin Sun , James Hays

Super-resolution (SR), the process of obtaining high-resolution images from one or more low-resolution observations of the same scene, has been a very popular topic of research in the last few decades in both signal processing and image…

Image and Video Processing · Electrical Eng. & Systems 2022-02-25 Bahattin Can Maral

Super-resolution (SR) is a technique that allows increasing the resolution of a given image. Having applications in many areas, from medical imaging to consumer electronics, several SR methods have been proposed. Currently, the best…

Computer Vision and Pattern Recognition · Computer Science 2019-12-02 Marija Vella , João F. C. Mota

Super-resolution is the process of obtaining a high-resolution image from one or more low-resolution images. Single image super-resolution (SISR) and multi-frame super-resolution (MFSR) methods have been evolved almost independently for…

Computer Vision and Pattern Recognition · Computer Science 2023-03-07 Mohammad Mahdi Afrasiabi , Reshad Hosseini , Aliazam Abbasfar

We develop an imaging algorithm that exploits strong scattering to achieve super-resolution in changing random media. The method processes large and diverse array datasets using sparse dictionary learning, clustering, and multidimensional…

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Super-resolution results are usually measured by full-reference image quality metrics or human rating scores. However, these evaluation methods are general image quality measurement, and do not account for the nature of the super-resolution…

Computer Vision and Pattern Recognition · Computer Science 2023-03-14 Sheng Cheng
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