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Biological macromolecules have complex and non-trivial energy landscapes, endowing them a unique conformational adaptability and diversity in function. Hence, understanding the processes of elasticity and dissipation at the nanoscale is…

Soft Condensed Matter · Physics 2009-11-11 Bhavin S. Khatri , Masaru Kawakami , Katherine Byrne , D. Alastair Smith , Tom C. B. McLeish

The glassy dynamics of soft harmonic spheres is often mapped onto the dynamics of hard spheres by considering an effective diameter for the soft particles and therefore an effective packing fraction. While in this approach the thermal…

Soft Condensed Matter · Physics 2019-02-25 Moumita Maiti , Michael Schmiedeberg

We present a molecular dynamics based method for computing accurately short-range structural forces resulting from the overlap of spatially diffuse solid-liquid interfaces at wetted grain boundaries close to the melting point. The method is…

Materials Science · Physics 2009-11-13 J. J. Hoyt , David Olmsted , Saryu Jindal , Mark Asta , Alain Karma

We review the background, theory and general equations for the analysis of equilibrium protein unfolding experiments, focusing on denaturant and heat-induced unfolding. The primary focus is on the thermodynamics of reversible…

Biological Physics · Physics 2020-12-23 Kresten Lindorff-Larsen , Kaare Teilum

Electron energy loss spectroscopy is consolidating as a powerful tool to explore electronic (as well as vibrational) excitations of matter, including molecules. Performed in a scanning transmission electron microscope, this technique is…

Chemical Physics · Physics 2021-03-05 Ciro A. Guido , Enzo Rotunno , Matteo Zanfrognini , Stefano Corni , Vincenzo Grillo

At equilibrium, thermodynamic and kinetic information can be extracted from biomolecular energy landscapes by many techniques. However, while static, ensemble techniques yield thermodynamic data, often only dynamic, single-molecule…

DNA hairpin molecules with periodic base sequences can be expected to exhibit a regular coarse-grained free energy landscape (FEL) as function of the number of open base pairs and applied mechanical force. Using a commonly employed model,…

Biological Physics · Physics 2013-12-17 Sandra Nostheide , Viktor Holubec , Petr Chvosta , Philipp Maass

We extend to finite temperature a Green's function method that was previously proposed to evaluate ground-state properties of mesoscopic clouds of non-interacting fermions moving under harmonic confinement in one dimension. By calculations…

Statistical Mechanics · Physics 2009-11-07 Z. Akdeniz , P. Vignolo , A. Minguzzi , M. P. Tosi

The use of beta-solenoid proteins as functionalizable, nanoscale, self-assembling molecular building blocks may have many applications, including templating the growth of wires or higher-dimensional structures. By understanding their…

Biomolecules · Quantitative Biology 2017-06-20 Amanda S. Parker , Krishnakumar M. Ravikumar , Daniel L. Cox

We consider a stiff polymer chain in poor solvent and apply a force at one end of the chain. We find that by varying the stiffness parameter, polymer undergoes a transition from the globule state to the folded like state. The conformation…

Soft Condensed Matter · Physics 2015-05-13 Sanjay Kumar , Debaprasad Giri

We propose a simple approach, based on the minimization of the total (entropic plus unfolding) energy of a two-state system, describing the stretch-induced unfolding of macromolecules (proteins, silks, nanopolymers, DNA/RNA). The model is…

Soft Condensed Matter · Physics 2013-04-03 Domenico De Tommasi , Nicola Millardi , Giuseppe Puglisi , Giuseppe Saccomandi

While ubiquitous, energy redistribution remains a poorly understood facet of the nonequilibrium thermodynamics of biomolecules. At the molecular level, finite-size effects, pronounced nonlinearities, and ballistic processes produce behavior…

Biological Physics · Physics 2019-11-15 Justin E. Elenewski , Kirill A. Velizhanin , Michael Zwolak

In spite of decades of research, much remains to be discovered about folding: the detailed structure of the initial (unfolded) state, vestigial folding instructions remaining only in the unfolded state, the interaction of the molecule with…

Biological Physics · Physics 2018-11-26 Walter A. Simmons

For several decades, experimental and computational studies have been used to investigate the potential functional role of knots in protein structures. A property that has attracted considerable attention is thermal stability, i.e., the…

Biomolecules · Quantitative Biology 2026-03-13 João N. C. Especial , Beatriz P. Teixeira , Ana Nunes , Miguel Machuqueiro , Patrícia F. N. Faísca

We analyze the thermodynamic properties of a simplified model for folded RNA molecules recently studied by G. Vernizzi, H. Orland, A. Zee (in {\it Phys. Rev. Lett.} {\bf 94} (2005) 168103). The model consists of a chain of one-flavor base…

Biomolecules · Quantitative Biology 2009-11-13 Matias dell'Erba , Guillermo R. Zemba

Experimental studies on enzyme evolution show that only a small fraction of all possible mutation trajectories are accessible to evolution. However, these experiments deal with individual enzymes and explore a tiny part of the fitness…

Populations and Evolution · Quantitative Biology 2015-05-30 Alexander E. Lobkovsky , Yuri I. Wolf , Eugene V. Koonin

Non-coding RNA sequences play a great role in controlling a number of cellular functions, thus raising the need to understand their complex conformational dynamics in quantitative detail. In this perspective, we first show that single…

Biomolecules · Quantitative Biology 2012-11-29 Jong-Chin Lin , Changbong Hyeon , D. Thirumalai

We show how to localize and quantify the functional evolutionary constraints on natural proteins. The method compares the perturbations caused by local sequence variants to the energetics of the protein folding process and to the…

Biomolecules · Quantitative Biology 2025-08-12 Ezequiel A. Galpern , Carlos Bueno , Ignacio E. Sánchez , Peter G. Wolynes , Diego U. Ferreiro

A method for reconstructing the energy landscape of simple polypeptidic chains is described. We show that we can construct an equivalent representation of the energy landscape by a suitable directed graph. Its topological and dynamical…

Soft Condensed Matter · Physics 2010-01-10 L. Bongini , L. Casetti , R. Livi , A. Politi , A. Torcini

Protein folding processes are generally described statistically with the help of multidimensional free energy landscape, typically reduced to a 1-D free energy profile along good reaction co-ordinate. There are many physical parameters…

Biological Physics · Physics 2017-05-04 Debajyoti De , Anurag Singh , Amar Nath Gupta
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