Linking thermodynamics and measurements of protein stability
Biological Physics
2020-12-23 v2 Chemical Physics
Abstract
We review the background, theory and general equations for the analysis of equilibrium protein unfolding experiments, focusing on denaturant and heat-induced unfolding. The primary focus is on the thermodynamics of reversible folding/unfolding transitions and the experimental methods that are available for extracting thermodynamic parameters. We highlight the importance of modelling both how the folding equilibrium depends on a perturbing variable such as temperature or denaturant concentration, and the importance of modelling the baselines in the experimental observables.
Keywords
Cite
@article{arxiv.2010.12281,
title = {Linking thermodynamics and measurements of protein stability},
author = {Kresten Lindorff-Larsen and Kaare Teilum},
journal= {arXiv preprint arXiv:2010.12281},
year = {2020}
}
Comments
21 pages, 5 figures