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We train a neural network to predict distributional responses in gene expression following genetic perturbations. This is an essential task in early-stage drug discovery, where such responses can offer insights into gene function and inform…

Gene regulatory networks, as a powerful abstraction for describing complex biological interactions between genes through their expression products within a cell, are often regarded as virtually deterministic dynamical systems. However, this…

Molecular Networks · Quantitative Biology 2023-09-18 Ulysse Herbach

Gene expression is a stochastic process governed by the presence of specific transcription factors. Here we study the dynamics of gene expression in the presence of feedback, where a gene regulates its own expression. The nonlinear coupling…

Cell Behavior · Quantitative Biology 2008-07-23 Otto Pulkkinen , Johannes Berg

Stochastic modeling of transcription is a classic yet long-standing problem in theoretical biophysics. The lack of unified results and a computationally efficient approach for a general, fine-grained transcription model has confined…

Biological Physics · Physics 2025-11-17 Yuntao Lu , Yunxin Zhang

Signal-processing molecules inside cells are often present at low copy number, which necessitates probabilistic models to account for intrinsic noise. Probability distributions have traditionally been found using simulation-based approaches…

Molecular Networks · Quantitative Biology 2009-11-09 Andrew Mugler , Aleksandra M. Walczak , Chris H. Wiggins

We introduce a biologically detailed, stochastic model of gene expression describing the multiple rate-limiting steps of transcription, nuclear pre-mRNA processing, nuclear mRNA export, cytoplasmic mRNA degradation and translation of mRNA…

Molecular Networks · Quantitative Biology 2024-01-24 Muhan Ma , Juraj Szavits-Nossan , Abhyudai Singh , Ramon Grima

We explore a Markov model used in the analysis of gene expression, involving the bursty production of pre-mRNA, its conversion to mature mRNA, and its consequent degradation. We demonstrate that the integration used to compute the solution…

Methodology · Statistics 2020-09-09 Gennady Gorin , Lior Pachter

Expression of many genes varies as a cell transitions through different cell-cycle stages. How coupling between stochastic expression and cell cycle impacts cell-to-cell variability (noise) in the level of protein is not well understood. We…

Molecular Networks · Quantitative Biology 2016-05-10 Mohammad Soltani , Abhyudai Singh

From the response to external stimuli to cell division and death, the dynamics of living cells is based on the expression of specific genes at specific times. The decision when to express a gene is implemented by the binding and unbinding…

Molecular Networks · Quantitative Biology 2009-11-13 Johannes Berg

Understanding how stochastic gene expression is regulated in biological systems using snapshots of single-cell transcripts requires state-of-the-art methods of computational analysis and statistical inference. A Bayesian approach to…

Quantitative Methods · Quantitative Biology 2018-12-10 Yen Ting Lin , Nicolas E. Buchler

Transcription commonly occurs in bursts, with alternating productive (ON) and quiescent (OFF) periods, governing mRNA production rates. Yet, how transcription is regulated through bursting dynamics remains unresolved. Here, we conduct…

Molecular Networks · Quantitative Biology 2025-11-11 Po-Ta Chen , Michal Levo , Benjamin Zoller , Thomas Gregor

Time delays, modelling the process of intracellular gene expression, have been shown to have important impacts on the dynamics of pattern formation in reaction-diffusion systems. In particular, past work has shown that such time delays can…

Pattern Formation and Solitons · Physics 2022-06-28 Alec Sargood , Eamonn A. Gaffney , Andrew L. Krause

Cell growth and gene expression, essential elements of all living systems, have long been the focus of biophysical interrogation. Advances in single-cell methods have invigorated theoretical studies into these processes. However, until…

Subcellular Processes · Quantitative Biology 2023-11-22 Ido Golding , Ariel Amir

In a general stochastic multistate promoter model of dynamic mRNA/protein interactions, we identify the stationary joint distribution of the promoter state, mRNA, and protein levels through an explicit `stick-breaking' construction of…

Statistics Theory · Mathematics 2021-08-26 William Lippitt , Sunder Sethuraman , Xueying Tang

Gene expression is a fundamental process in a living system. The small RNAs (sRNAs) is widely observed as a global regulator in gene expression. The inherent nonlinearity in this regulatory process together with the bursty production of…

Molecular Networks · Quantitative Biology 2021-10-12 Shigang Qiu , Tao Jia

In this paper we develop a model of stochastic gene expression, which is an extension of the model investigated in the paper [T. Lipniacki, P. Paszek, A. Marciniak-Czochra, A.R. Brasier, M. Kimmel, Transcriptional stochasticity in gene…

Probability · Mathematics 2015-10-20 Ryszard Rudnicki , Andrzej Tomski

Stochastic oscillations in individual cells are usually characterized by a non-monotonic power spectrum with an oscillatory autocorrelation function. Here we develop an analytical approach of stochastic oscillations in a minimal hybrid…

Molecular Networks · Quantitative Biology 2024-02-08 Chen Jia , Hong Qian , Michael Q. Zhang

The rate of mRNA translation depends on the initiation, elongation, and termination rates of ribosomes along the mRNA. These rates depend on many "local" factors like the abundance of free ribosomes and tRNA molecules in the vicinity of the…

Subcellular Processes · Quantitative Biology 2021-02-25 Michael Margaliot , Wasim Huleihel , Tamir Tuller

Gene expression time delays, modelling the complex biological processes of gene transcription and translation, have been shown to play an important role in cellular dynamics. Time delays, motivated by the gene expression process, can also…

Cell Behavior · Quantitative Biology 2022-02-24 Alec Sargood

We derive exact solutions of simplified models for the temporal evolution of the protein concentration within a cell population arbitrarily far from the stationary state. We show that monitoring the dynamics can assist in modeling and…

Biomolecules · Quantitative Biology 2015-05-13 Sandro Azaele , Jayanth R. Banavar , Amos Maritan