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We present a novel approach for extracting 3D atomic-level information from transmission electron microscopy (TEM) images affected by significant noise. The approach is based on formulating depth estimation as a semantic segmentation…

Computer Vision and Pattern Recognition · Computer Science 2026-01-29 Matan Leibovich , Mai Tan , Ramon Manzorro , Adria Marcos-Morales , Sreyas Mohan , Peter A. Crozier , Carlos Fernandez-Granda

The work proposes an image segmentation algorithm that isolates slender regions in three-dimensional microstructures. Characterizing slender regions in material microstructures is an extremely important aspect in material science because…

Data Analysis, Statistics and Probability · Physics 2025-03-13 Vinit Vijay Deshpande , Romana Piat

Cryo-electron microscopy (cryo-EM) studies using single particle reconstruction is extensively used to reveal structural information of macromolecular complexes. Aiming at the highest achievable resolution, state of the art electron…

Computer Vision and Pattern Recognition · Computer Science 2020-05-04 Ramin Norousi , Stephan Wickles , Thomas Becker , Roland Beckmann , Volker J. Schmid , Achim Tresch

Traditional iterative reconstruction methods are accurate but computationally expensive, limiting their use in high-throughput and real-time ptychography. Recent deep learning approaches improve speed, but often predict phase as a Euclidean…

Image and Video Processing · Electrical Eng. & Systems 2026-04-30 Carson Yu Liu , Jun Cheng , Chien-Chun Chen , Steve F. Shu

Tissue stiffness is related to soft tissue pathologies and can be assessed through palpation or via clinical imaging systems, e.g., ultrasound or magnetic resonance imaging. Typically, the image based approaches are not suitable during…

Signal Processing · Electrical Eng. & Systems 2025-09-04 Maximilian Neidhardt , Sarah Latus , Tim Eixmann , Gereon Hüttmann , Alexander Schlaefer

Advances in image registration and machine learning have recently enabled volumetric analysis of postmortem brain tissue from conventional photographs of coronal slabs, which are routinely collected in brain banks and neuropathology…

The dynamic analysis of erythrocyte deformability is used as an important means for early diagnosis of blood diseases and blood rheology. Yet no effective method is available in terms of three-dimensional reconstruction of erythrocytes in a…

Medical Physics · Physics 2013-06-26 Yifang Fan , Yubo Fan , Zhiyu Li , Wentao Lin , Yuan Wei , Xing Zhong , Tony Newman , Changsheng Lv , Yuzhou Fan

In this paper we propose a deep learning approach for segmenting sub-cortical structures of the human brain in Magnetic Resonance (MR) image data. We draw inspiration from a state-of-the-art Fully-Convolutional Neural Network (F-CNN)…

Computer Vision and Pattern Recognition · Computer Science 2016-02-08 Mahsa Shakeri , Stavros Tsogkas , Enzo Ferrante , Sarah Lippe , Samuel Kadoury , Nikos Paragios , Iasonas Kokkinos

Three dimensional electron back-scattered diffraction (EBSD) microscopy is a critical tool in many applications in materials science, yet its data quality can fluctuate greatly during the arduous collection process, particularly via…

Computer Vision and Pattern Recognition · Computer Science 2023-08-21 Harry Dong , Sean Donegan , Megna Shah , Yuejie Chi

Here we apply hyperspectral bright field imaging to collect computed tomographic images with excellent energy resolution (800 eV), applying it for the first time to map the distribution of stain in a fixed biological sample through its…

Ptychography spans from sub-angstrom to meter scales yet suffers from convergence instability and excessive data redundancy. Here we introduce self-correcting residual neural fields as a dose-efficient framework for electron, X-ray, and…

Limited-angle electron tomography aims to reconstruct 3D shapes from 2D projections of Transmission Electron Microscopy (TEM) within a restricted range and number of tilting angles, but it suffers from the missing-wedge problem that causes…

Computer Vision and Pattern Recognition · Computer Science 2026-05-05 Zhantao Deng , Mériem Er-Rafik , Anna Sushko , Cécile Hébert , Pascal Fua

Scanning electron microscopy (SEM), a century-old technique, is today a ubiquitous method of imaging the surface of nanostructures. However, most SEM detectors simply count the number of secondary electrons from a material of interest, and…

Accurate segmentation and measurement of lithography scanning electron microscope (SEM) images are crucial for ensuring precise process control, optimizing device performance, and advancing semiconductor manufacturing yield. Lithography…

Computer Vision and Pattern Recognition · Computer Science 2025-11-18 Xinyu He , Botong Zhao , Bingbing Li , Shujing Lyu , Jiwei Shen , Yue Lu

Optical phase contains key information for biomedical and astronomical imaging. However, it is often obscured by layers of heterogeneous and scattering media, which render optical phase imaging at different depths an utmost challenge.…

Image restoration of biological structures in microscopy poses unique challenges for preserving fine textures and sharp edges. While recent GAN-based image restoration formulations have introduced frequency-domain losses for natural images,…

Quantitative Methods · Quantitative Biology 2026-01-30 Xingjian Zhang , Claire Leclech , Louison Blivet-Bailly , Abdul I. Barakat , Elsa D. Angelini

Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…

Electromagnetic (EM) imaging is an important tool for non-invasive sensing with low-cost and portable devices. One emerging application is EM stroke imaging, which enables early diagnosis and continuous monitoring of brain strokes.…

Signal Processing · Electrical Eng. & Systems 2025-09-08 Rui Guo , Yi Zhang , Yhonatan Kvich , Tianyao Huang , Maokun Li , Yonina C. Eldar

The measurement of cellular traction forces on soft elastic substrates has become a standard tool for many labs working on mechanobiology. Here we review the basic principles and different variants of this approach. In general, the…

Quantitative Methods · Quantitative Biology 2015-06-09 Ulrich S. Schwarz , Jerome R. D. Soine

Constructing 3D structures from serial section data is a long standing problem in microscopy. The structure of a fiber reinforced composite material can be reconstructed using a tracking-by-detection model. Tracking-by-detection algorithms…

Computer Vision and Pattern Recognition · Computer Science 2018-05-28 Hongkai Yu , Dazhou Guo , Zhipeng Yan , Wei Liu , Jeff Simmons , Craig P. Przybyla , Song Wang
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