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Increasing demand for understanding the vast heterogeneity of cellular phenotypes has driven the development of imaging flow cytometry (IFC), that combines features of flow cytometry with fluorescence and bright field microscopy. IFC…

Quantitative Methods · Quantitative Biology 2019-02-06 Yuanyuan Han , Rui Tang , Yi Gu , Alex Ce Zhang , Wei Cai , Violet Castor , Sung Hwan Cho , William Alaynick , Yu-Hwa Lo

We describe an instrument which can be used to analyze complex chemical mixtures at high resolution and high sensitivity. Molecules are collisionally cooled with helium gas at cryogenic temperatures (~ 4-7 K), and subsequently detected…

Instrumentation and Detectors · Physics 2019-05-22 Jessica P. Porterfield , Lincoln Satterthwaite , Sandra Eibenberger , David Patterson , Michael C. McCarthy

We propose to use a compound of magnetic nanoparticles (20-100 nm) embedded in a flexible polymer (Polydimethylsiloxane PDMS) to filter circulating tumor cells (CTCs). The analysis of CTCs is an emerging tool for cancer biology research and…

Quantum simulation relies on the preparation and control of low-entropy many-body systems to reveal the behavior of classically intractable models. The development of new approaches for realizing such systems therefore represents a frontier…

Identifying pathogens in complex samples such as blood, urine, and wastewater is critical to detect infection and inform optimal treatment. Surface-enhanced Raman spectroscopy (SERS) and machine learning (ML) can distinguish among multiple…

Bacteria sense a diverse range of environmental analytes with high sensitivity and temporal resolution. Engineering and synthetic biology approaches enabled harnessing this capability through development of whole-cell biosensors that…

We report on the development of some process capabilities for a polymer-based, multi-layer microelectrofluidic platform, namely: the hot embossing process, metallization on polymer and polymer bonding. Hot embossing experiments were…

Other Computer Science · Computer Science 2007-11-29 S. -H. Ng , Z. -F. Wang , R. -T. Tjeung , N. De Rooij

We present a multiplexing scheme for the measurement of large numbers of mesoscopic devices in cryogenic systems. The multiplexer is used to contact an array of 256 split gates on a GaAs/AlGaAs heterostructure, in which each split gate can…

Mesoscale and Nanoscale Physics · Physics 2013-06-19 H. Al-Taie , L. W. Smith , B. Xu , P. See , J. P. Griffiths , H. E. Beere , G. A. C. Jones , D. A. Ritchie , M. J. Kelly , C. G. Smith

The next generation of proton decay and neutrino experiments, the post-SuperKamiokande detectors as those that will take place in megaton size water tanks, will require very large surfaces of photodetection and a large volume of data. Even…

The fabrication and experimental characterization of a thermal flow meter, capable of detecting and measuring two independent gas flows with a single chip, is described. The device is based on a 4 x 4 mm2 silicon chip, where a series of…

Other Computer Science · Computer Science 2008-12-18 P. Bruschi , M. Dei , M. Piotto

We suggest a new multimodal imaging technique for quantitatively measuring the integral (thickness-average) refractive index of the nuclei of live biological cells in suspension. For this aim, we combined quantitative phase microscopy with…

Bright-field microscopy, a cost-effective solution for live-cell culture, is often the only resource available, along with standard CPUs, for many low-budget labs. The inherent challenges of bright-field images -- their noisiness, low…

Quantitative Methods · Quantitative Biology 2025-11-03 Surajit Das , Pavel Zun

We introduce a scalable witness-based persistent homology pipeline for full-brain MRI volumes that couples density-aware landmark selection with a GPU-ready witness filtration. Candidates are scored by a hybrid metric that balances…

Computational Geometry · Computer Science 2025-10-07 Jorge Leonardo Ruiz Williams

In the clinic, resected tissue samples are stained with Hematoxylin-and-Eosin (H&E) and/or Immunhistochemistry (IHC) stains and presented to the pathologists on glass slides or as digital scans for diagnosis and assessment of disease…

Computer Vision and Pattern Recognition · Computer Science 2022-04-12 Parmida Ghahremani , Joseph Marino , Ricardo Dodds , Saad Nadeem

Dermal interstitial fluid (ISF) is a rich source of biomarkers (e.g., glucose) that can be used for continuous health monitoring with wearable sensors. Hollow microneedle devices are a promising solution to extract ISF on demand by…

We present a new and versatile implementation of rapid and localized immunohistochemical staining of tissue sections. Immunohistochemistry (IHC) allows to detect specific proteins on tissue sections and comprises a sequence of specific…

Tissues and Organs · Quantitative Biology 2021-05-18 Robert D. Lovchik , David Taylor , Govind Kaigala

Progress in superconducting qubit experiments with greater numbers of qubits or advanced techniques such as feedback requires faster and more accurate state measurement. We have designed a multiplexed measurement system with a bandpass…

Producing images from interferometer data requires accurate modeling of the sources in the field of view, which is typically done using the CLEAN algorithm. Given the large number of degrees of freedom in interferometeric images, one…

Instrumentation and Methods for Astrophysics · Physics 2020-01-22 Amanda A. Kepley , Takahiro Tsutsumi , Crystal L. Brogan , Remy Indebetouw , Ilsang Yoon , Brian Mason , Jennifer Donovan Meyer

Inexpensive, portable lab-on-a-chip devices would revolutionize fields like environmental monitoring and global health, but current microfluidic chips are tethered to extensive off-chip hardware. Insects, however, are self-contained and…

Proteinoids are solidified gels made from poly(amino acids) based polymers that exhibit oscillatory electrical activity. It has been proposed that proteinoids are capable of performing analog computing as their electrical activity can be…