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We describe a two-beam interference structured illumination fluorescence microscope. The novelty of the presented system lies in its simplicity. A programmable electro-optical spatial light modulator in an intermediate image plane enables…

We report an angle-tilted, wavelength-multiplexed ptychographic modulation approach for multispectral lensless on-chip microscopy. In this approach, we illuminate the specimen with lights at 5 wavelengths simultaneously. A prism is added at…

This paper is concerned with investigating super-resolution algorithms and solutions for handling electron microscopic images. We note two main aspects differentiating the problem discussed here from those considered in the literature. The…

Image and Video Processing · Electrical Eng. & Systems 2020-08-26 Yanjun Qian , Jiaxi Xu , Lawrence F. Drummy , Yu Ding

Deep learning has become an extremely effective tool for image classification and image restoration problems. Here, we apply deep learning to microscopy, and demonstrate how neural networks can exploit the chromatic dependence of the…

Optics · Physics 2018-07-05 Eran Hershko* , Lucien E. Weiss* , Tomer Michaeli , Yoav Shechtman

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

I explain what is, what is not, and what is only sort of superresolution microscopy. I discuss optical resolution, first in terms of diffraction theory, then in terms of linear systems theory, and finally in terms of techniques that use…

Optics · Physics 2015-04-02 John Bechhoefer

Tracking single fluorescent molecules has offered resolution into dynamic molecular processes at the single-molecule level. This perspective traces the evolution of single-molecule tracking, highlighting key developments across various…

Biological Physics · Physics 2026-02-24 Lance W. Q. Xu , Steve Pressé

A microscopic theory for the luminescence of ordered semiconductors is modified to describe photoluminescence of strongly disordered semiconductors. The approach includes both diagonal disorder and the many-body Coulomb interaction. As a…

Disordered Systems and Neural Networks · Physics 2007-08-22 P. Bozsoki , M. Kira , W. Hoyer , T. Meier , I. Varga , P. Thomas , S. W. Koch

Deep learning methods have been successfully applied to various computer vision tasks. However, existing neural network architectures do not per se incorporate domain knowledge about the addressed problem, thus, understanding what the model…

Computer Vision and Pattern Recognition · Computer Science 2019-10-21 Iman Marivani , Evaggelia Tsiligianni , Bruno Cornelis , Nikos Deligiannis

Solid-state quantum emitters coupled to integrated photonic nanostructures are quintessential for exploring fundamental phenomena in cavity quantum electrodynamics and widely employed in photonic quantum technologies such as non-classical…

The phase-resolved imaging of confined light fields by homodyne detection is a cornerstone of metrology in nano-optics and photonics, but its application in electron microscopy has been limited so far. Here, we report the mapping of optical…

Mesoscale and Nanoscale Physics · Physics 2024-08-27 Jonathan T. Weber , Niklas Müller , Alexander Schröder , Sascha Schäfer

Super-resolution aims at increasing image resolution by algorithmic means and has progressed over the recent years due to advances in the fields of computer vision and deep learning. Convolutional Neural Networks based on a variety of…

Image and Video Processing · Electrical Eng. & Systems 2020-08-11 M. U. Müller , N. Ekhtiari , R. M. Almeida , C. Rieke

Precise control and manipulation of optical fields on a nanoscale is one of the most important and challenging problems in "nanophotonics". Since optical wavelength is on a much larger microscale, it is impossible to employ conventional…

Optics · Physics 2011-06-03 T. S. Kao , S. D. Jenkins , J. Ruostekoski , N. I. Zheludev

Deep learning algorithms offer a powerful means to automatically analyze the content of medical images. However, many biological samples of interest are primarily transparent to visible light and contain features that are difficult to…

Computer Vision and Pattern Recognition · Computer Science 2017-09-22 Roarke Horstmeyer , Richard Y. Chen , Barbara Kappes , Benjamin Judkewitz

Single-beam scanning microscopy (SBSM) is one of the most robust strategies for commercial optical systems. Although structured illumination combined with Fourier-domain spatial spectrum fusion can enhance SBSM resolution beyond the…

Optics · Physics 2025-07-15 Yongtao Liu , Tianxiao Wu , Xiao Zhou , Fan Wang

Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular localization tools to study molecular structure at the nanoscale level in the intact cell, bridging the mesoscale gap to classical structural biology…

Subcellular Processes · Quantitative Biology 2024-05-28 Ivan R. Nabi , Ben Cardoen , Ismail M. Khater , Guang Gao , Timothy H. Wong , Ghassan Hamarneh

Super-resolution imaging is vital for optical applications, such as high capacity information transmission, real-time bio-molecular imaging and nanolithography. Technology and method of super-resolution imaging have attracted much…

Optics · Physics 2022-10-19 Yangyang Zhou , Huanyang Chen

Single image super-resolution (SISR) is a very popular topic nowadays, which has both research value and practical value. In daily life, we crop a large image into sub-images to do super-resolution and then merge them together. Although…

Computer Vision and Pattern Recognition · Computer Science 2019-07-31 Junyu , Wang , Rong Song

We demonstrate spectroscopy of incoherent light with sub-diffraction resolution. In a proof-of-principle experiment we analyze the spectrum of a pair of incoherent point-like sources whose separation is below the diffraction limit. The two…

Non-destructive nano-imaging of the internal structure of solid matter is only feasible using hard X-rays due to their high penetration. The highest resolution images are achieved at synchrotron radiation sources (SRF), offering superior…