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In this paper, we review spatial light interference microscopy (SLIM), a common-path, phase-shifting interferometer, built onto a phase-contrast microscope, with white-light illumination. As one of the most sensitive quantitative phase…
Photothermal spectroscopy and microscopy provides a route to measure the spectral and spatial properties of individual nanoscopic absorbers, independent from scattering, extinction, and emission. The approach relies upon use of two light…
We demonstrate spectroscopy of incoherent light with sub-diffraction resolution. In a proof-of-principle experiment we analyze the spectrum of a pair of incoherent point-like sources whose separation is below the diffraction limit. The two…
The design is suggested, and possible operation parameters are discussed, of an instrument to inspect a skin cancer tumour in the terahertz (THz) range, transferring the image into the infrared (IR) and making it visible with the help of…
Image sensors are the backbone of many imaging technologies of great importance to modern sciences, being particularly relevant in biomedicine. An ideal image sensor should be usable through all the electromagnetic spectrum (large…
In the 21st century, new and alternative non-radioactive methods for cancer treatment have been developed. One of this is magnetic hyperthermia (MH), which is based on the ability of magnetic nanoparticles (MNP) to transduce electromagnetic…
We present a simple and inexpensive means to measure background-free photoinduced absorption spectra by implementing a dual optical modulation scheme. Our objective is to measure quasi-steady-state absorption spectra of long-lived…
Two blind source separation methods (Independent Component Analysis and Non-negative Matrix Factorization), developed initially for signal processing in engineering, found recently a number of applications in analysis of large-scale data in…
Infrared (IR) dyes, especially those within the near-infrared (NIR) spectrum, offer substantial advantages for in vivo imaging, owing to their deep tissue penetration and minimal background autofluorescence. Nanoparticles incorporating…
Extracting colorimetric image information from the spectral characteristics of image sensors is a key issue in accurate image acquisition. Technically feasible filter/sensor combinations usually do not replicate colorimetric responses with…
Hyperspectral imaging is useful for applications ranging from medical diagnostics to agricultural crop monitoring; however, traditional scanning hyperspectral imagers are prohibitively slow and expensive for widespread adoption. Snapshot…
Purpose: The terahertz (THz) medical imaging is a promising noninvasive technique for monitoring the skin's conditions, early detection of the human skin cancer, and recovery from burns and wounds. It can be applied for visualization of…
The wavelength calibration of spectrographs is an essential but challenging task in many disciplines. Calibration is traditionally accomplished by imaging the spectrum of a light source containing features that are known to appear at…
Fourier ptychographic (FP) microscope is a coherent imaging method that can synthesize an image with a higher bandwidth using multiple low-bandwidth images captured at different spatial frequency regions. The method's demand for multiple…
Chemical imaging provides information about the distribution of chemicals within a target. When combined with structural information about the target, in situ chemical imaging opens the door to applications ranging from tissue…
Different imaging modalities are used to extract the diverse information carried in an optical field. Two prominent modalities include bright field and phase contrast microscopy that can visualize the amplitude and phase features of a…
Photothermal-Induced Resonance (PTIR) spectroscopy and imaging with infrared light has seen increasing application in molecular spectroscopy of biological samples. The appeal of the technique lies in its capability to provide information…
Spatial light modulators enable arbitrary control of the intensity of optical light fields and facilitate a variety of applications in biology, astronomy and atomic, molecular and optical physics. For coherent light fields, holography,…
Biological soft tissues encountered in clinical and pre-clinical imaging mainly consist of light element atoms, and their composition is nearly uniform with little density variation. Thus, x-ray attenuation imaging suffers from low image…
The utilization of computational photography becomes increasingly essential in the medical field. Today, imaging techniques for dermatology range from two-dimensional (2D) color imagery with a mobile device to professional clinical imaging…