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Fluorescence microscopy is indispensable in nanoscience and biological sciences. The versatility of labeling target structures with fluorescent dyes permits to visualize structure and function at a subcellular resolution with a wide field…

Fluorescence molecular tomography (FMT) is a sensitive optical imaging technology widely used in biomedical research. However, the ill-posedness of the inverse problem poses a huge challenge to FMT reconstruction. Although end-to-end deep…

Image and Video Processing · Electrical Eng. & Systems 2025-03-18 Peng Zhang , Qianqian Xue , Xingyu Liu , Guanglei Zhang , Wenjian Wang , Jiye Liang

Spectral imaging is a fundamental diagnostic technique with widespread application. Conventional spectral imaging approaches have intrinsic limitations on spatial and spectral resolutions due to the physical components they rely on. To…

Image and Video Processing · Electrical Eng. & Systems 2021-06-07 Figen S. Oktem , Oğuzhan Fatih Kar , Can Deniz Bezek , Farzad Kamalabadi

Tilting planar samples for multi-zone-axes observation is a routine procedure in electron microscopy. However, this process invariably introduces optical path differences in the electron beam across different sample positions, significantly…

Materials Science · Physics 2024-08-22 Jizhe Cui , Yi Zheng , Kang Sun , Wenfeng Yang , Haozhi Sha , Rong Yu

Mid-infrared photo-induced force microscopy (IR-PiFM/PiF-IR) enables high-resolution chemical imaging of surfaces with lateral resolution less than 5 nm. Here are some answers to questions about the physical background, practical handling…

Materials Science · Physics 2026-05-05 Daniela Täuber

Diffraction tomography is an inverse scattering technique used to reconstruct the spatial distribution of the material properties of a weakly scattering object. The object is exposed to radiation, typically light or ultrasound, and the…

Numerical Analysis · Mathematics 2024-03-26 Clemens Kirisits , Noemi Naujoks , Otmar Scherzer

Lens-free on-chip digital holographic microscopy (LFOCDHM) is a modern imaging technique whereby the sample is placed directly onto or very close to the digital sensor, and illuminated by a partially coherent source located far above it.…

Optics · Physics 2019-06-17 Jialin Zhang , Jiasong Sun , Qian Chen , Chao Zuo

The plug-and-play priors (PnP) framework has been recently shown to achieve state-of-the-art results in regularized image reconstruction by leveraging a sophisticated denoiser within an iterative algorithm. In this paper, we propose a new…

Computer Vision and Pattern Recognition · Computer Science 2021-03-25 Yu Sun , Shiqi Xu , Yunzhe Li , Lei Tian , Brendt Wohlberg , Ulugbek S. Kamilov

We present two complementary algorithms suitable for using focal-plane measurements to control a wavefront corrector with an extremely high spatial resolution. The algorithms use linear approximations to iteratively minimize the aberrations…

Instrumentation and Methods for Astrophysics · Physics 2015-06-19 Visa Korkiakoski , Christoph U. Keller , Niek Doelman , Matthew Kenworthy , Gilles Otten , Michel Verhaegen

The goal of synthetic aperture imaging is to estimate the reflectivity of a remote region of interest by processing data gathered with a moving sensor which emits periodically a signal and records the backscattered wave. We introduce and…

Image and Video Processing · Electrical Eng. & Systems 2019-07-05 Liliana Borcea , Josselin Garnier

A hybrid imaging system is a simultaneous physical arrangement of a refractive lens and a multilevel phase mask (MPM) as a diffractive optical element (DOE). The favorable properties of the hybrid setup are improved extended-depth-of-field…

Neurological Positron Emission Tomography (PET) is a critical imaging modality for diagnosing and studying neurodegenerative diseases like Alzheimer's disease. However, the inherent low spatial resolution of PET images poses significant…

Medical Physics · Physics 2025-02-24 Matthew Tivnan , Quanzheng Li

Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…

A light field print (LFP) displays three-dimensional (3D) information to the naked-eye observer under ambient white light illumination. Changing perspectives of a 3D image are seen by the observer from varying angles. However, LFPs appear…

A new far-field optical microscopy technique capable of reaching nanometer-scale resolution has been developed recently using the in-plane image magnification by surface plasmon polaritons. This microscopy is based on the optical properties…

Strongly Correlated Electrons · Physics 2009-11-10 Igor I. Smolyaninov

The ability to resolve detail in the object that is being imaged, named by resolution, is the core parameter of an imaging system. Super-resolution is a class of techniques that can enhance the resolution of an imaging system and even…

Data Structures and Algorithms · Computer Science 2022-10-13 Yaonan Jin , Daogao Liu , Zhao Song

Dark-field X-ray microscopy is a new full-field imaging technique that nondestructively maps the structure and local strain inside deeply embedded crystalline elements in three dimensions. Placing an objective lens in the diffracted beam…

Materials Science · Physics 2022-03-10 H. F. Poulsen , L. E. Dresselhaus-Marais , M. A. Carlsen , C. Detlefs , G. Winther

We present a structured-illumination technique for full-field super-resolution transmission X-ray microscopy, which employs Fourier spectral decomposition inspired by established methods in visible-light microscopy. A 2D grating creating…

Traditional fluorescence microscopy is constrained by inherent trade-offs among resolution, field-of-view, and system complexity. To navigate these challenges, we introduce a simple and low-cost computational multi-aperture miniature…

Optics · Physics 2024-05-31 Qianwan Yang , Ruipeng Guo , Guorong Hu , Yujia Xue , Yunzhe Li , Lei Tian

Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…

Optics · Physics 2025-01-31 Oumeng Zhang , Changhuei Yang