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Camera calibration is an essential process in photogrammetry, serving as a crucial link between the 2D image coordinate system and the 3D world coordinate system. However, when observations are conducted through refractive interfaces, the…

Many adaptive optics systems operate by measuring the distortion of the wavefront in one wavelength range and performing the scientific observations in a second, different wavelength range. One common technique is to measure wavefront…

Astrophysics · Physics 2009-11-07 Henry G. Roe

Robust and accurate camera calibration is essential for 3D reconstruction in light microscopy under circular motion. Conventional methods require either accurate key point matching or precise segmentation of the axial-view images. Both…

Computer Vision and Pattern Recognition · Computer Science 2019-10-31 Yuanhao Guo , Fons J. Verbeek , Ge Yang

The technique of remote refocusing is used in optical microscopy to provide rapid axial scanning without mechanically perturbing the sample and in techniques such as oblique plane microscopy that build on remote refocusing to image a tilted…

Optics · Physics 2023-09-27 Wenzhi Hong , Hugh Sparks , Chris Dunsby

We report on a method to obtain confocal imaging through multimode fibers using optical correlation. First, we measure the fiber's transmission matrix in a calibration step. This allows us to create focused spots at one end of the fiber by…

Deconvolution phase microscopy enables high-contrast visualization of transparent samples through reconstructions of their transmitted phases or refractive indexes. Herein, we propose a method to extend 2D deconvolution phase microscopy to…

Optics · Physics 2022-04-20 Herve Hugonnet , Mahn Jae Lee , Yongkeun Park

Parallax measurements allow distances to celestial objects to be determined. Coupled with measurement of their position on the celestial sphere, it gives a full three-dimensional picture of the location of the objects relative to the…

Astrophysics · Physics 2015-06-24 M. V. Sazhin , V. E. Zharov , T. A. Kalinina

Unmeasured, spatially-structured factors can confound associations between spatial environmental exposures and health outcomes. Adding flexible splines to a regression model is a simple approach for spatial confounding adjustment, but the…

Applications · Statistics 2020-06-22 Joshua P. Keller , Adam A. Szpiro

Remote focusing (RF) is a technique that greatly extends the aberration-free axial scan range of an optical microscope. To maximise the diffraction limited depth range in an RF system, the magnification of the relay lenses should be such…

Optics · Physics 2021-01-05 Sharika Mohanan , Alexander D. Corbett

Underwater cameras are typically placed behind glass windows to protect them from the water. Spherical glass, a dome port, is well suited for high water pressures at great depth, allows for a large field of view, and avoids refraction if a…

Computer Vision and Pattern Recognition · Computer Science 2021-12-15 Mengkun She , David Nakath , Yifan Song , Kevin Köser

We compared several techniques for calibrating angular separation between wide (>1 arcsec) pairs. These techniques are (i) reference pair calibration using {\alpha} Cen AB orbital parameters, (ii) the video drift method, and (iii) the…

Instrumentation and Methods for Astrophysics · Physics 2021-05-13 Matthew B. James , Graeme L. White , Stephen G. Bosi , Rod R. Letchford

The complete characterization of spatial coherence is difficult because the mutual coherence function is a complex-valued function of four independent variables. This difficulty limits the ability of controlling and optimizing spatial…

For cone-beam three-dimensional computed tomography (3D-CT) scanning system, voxel size is an important indicator to guarantee the accuracy of data analysis and feature measurement based on 3D-CT images. Meanwhile, the voxel size changes…

Instrumentation and Detectors · Physics 2015-06-15 Min Yang , Xiaolong Wang , Yipeng Liu , Fanyong Men , Xingdong Li , Wenli Liu , Dongbo Wei

We propose a straightforward sample-based technique to calibrate the axial detection in 3D single molecule localization microscopy (SMLM). Using microspheres coated with fluorescent molecules, the calibration curves of PSF-shaping- or…

Fluorescence Correlation Spectroscopy (FCS) yields measurement parameters (number of molecules, diffusion time) that characterize the concentration and kinetics of fluorescent molecules within a supposedly known observation volume. Absolute…

Biological Physics · Physics 2011-12-23 Charles Edouard Leroux , Irène Wang , Jacques Derouard , Antoine Delon

Long cylinders, such as optical fibers, are some of the most widely used photonic devices. The radius and refractive index of these fibers are therefore fundamentally important parameters in determining their performance. We have developed…

Optics · Physics 2022-07-20 Mathieu Couillard , Pablo Bianucci

Refractive index is a fundamental optical property of powder and a key input to the measurement of the size distribution using light scattering and the measurement of the absorption and scattering coefficients using diffuse reflectance…

Optics · Physics 2024-03-05 Cory Juntunen , Adam J. Rish , Carl Anderson , Yongjin Sung

The computation time required by standard finite difference methods with fixed timesteps for solving fractional diffusion equations is usually very large because the number of operations required to find the solution scales as the square of…

Numerical Analysis · Mathematics 2024-06-28 Santos B. Yuste , Joaquin Quintana-Murillo

In absence of a lens to form an image, incoherent or partially coherent light scattering off an obstructive or reflective object forms a broad intensity distribution in the far field with only feeble spatial features. We show here that…

We propose a solution for increasing the axial resolution of confocal microscopes. In the experimental set-up described in this paper an interference phenomenon between two counterpropagating beams is used to determine the axial position of…

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