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In conventional optical microscopes, image contrast of objects mainly results from the differences in light intensity and/or color. Muller matrix optical microscopes (MMMs), on the other hand, can provide significantly enhanced image…

Molecular interactions are key to many chemical and biological processes like protein function. In many signaling processes they occur in sub-cellular areas displaying nanoscale organizations and involving molecular assemblies. The…

Nitrogen-vacancy (NV) centers show great potentials for nanoscale bio-sensing and bio-imaging. Nevertheless, their envisioned bio-applications suffer from intrinsic background noise due to unavoidable light scattering and autofluorescence…

Optics · Physics 2024-08-05 Yayin Tan , Xiaolu Wang , Feng Xu , Xinhao Hu , Yuan Lin , Bo Gao , Zhiqin Chu

Live cell imaging using metallic nanoparticles as tags is an emerging technique to visualize long and highly dynamic processes due to the lack of photobleaching and high photon rate. However, the lack of excited states as compared to…

Mesoscale and Nanoscale Physics · Physics 2011-01-12 H. E. Grecco , O. E. Martínez

Polymer-sorted high-density carbon nanotube (CNT) arrays have shown great potential to extend the silicon-based Moore's law. Imaging the CNT arrays on insulators like SiO2/Si using low-voltage scanning electron microscopy (LVSEM) to acquire…

Applied Physics · Physics 2023-06-06 Boxiang Zhang , Zhiyong Zhang , Chuanhong Jin

In situ measurement of analytes for in vivo or in vitro systems has been challenging due to the bulky size of traditional analytical instruments. Also, frequent in vitro concentration measurements rely on fluorescence-based methods or…

Light sources on the scale of single molecules can be addressed and characterized on their proper sub-nanometer scale by scanning tunneling microscopy induced luminescence (STML). Such a source can be driven by defined short charge pulses…

Mesoscale and Nanoscale Physics · Physics 2021-06-15 Anna Rosławska , Pablo Merino , Christopher C. Leon , Abhishek Grewal , Markus Etzkorn , Klaus Kuhnke , Klaus Kern

Low quantum yields of near infrared (NIR) fluorophores have limited their capabilities as imaging probes in a transparent, low background imaging window. Here for the first time we reported near-infrared fluorescence enhance (NIR-FE) cell…

This paper presents a comprehensive numerical analysis of super-resolution imaging using dielectric microparticles, employing the Finite-Difference Time-Domain (FDTD) method to elucidate the mechanisms that enable resolution enhancements…

Optics · Physics 2024-09-18 Arlen Bekirov , Zengbo Wang , Boris Lukyanchuk

Despite the significant progress in image denoising, it is still challenging to restore fine-scale details while removing noise, especially in extremely low-light environments. Leveraging near-infrared (NIR) images to assist visible RGB…

Computer Vision and Pattern Recognition · Computer Science 2025-03-11 Rongjian Xu , Zhilu Zhang , Renlong Wu , Wangmeng Zuo

The curvature of biological membranes at the nanometer scale is critically important for vesicle trafficking, organelle morphology, and disease propagation. This manuscript reports the development of Polarized Localization Microscopy (PLM),…

Biological Physics · Physics 2017-03-31 Abir Maarouf Kabbani , Christopher V. Kelly

Characterizing materials using electron micrographs is crucial in areas such as semiconductors and quantum materials. Traditional classification methods falter due to the intricatestructures of these micrographs. This study introduces an…

Computer Vision and Pattern Recognition · Computer Science 2024-08-28 Sakhinana Sagar Srinivas , Geethan Sannidhi , Sreeja Gangasani , Chidaksh Ravuru , Venkataramana Runkana

We present a coherent population trapping based scheme to attain sub-nanoscale resolution for atom localization, microscopy and lithography. Our method uses three-level atoms coupled to amplitude modulated probe field and spatially…

Quantum Physics · Physics 2009-12-08 K. T. Kapale , G. S. Agarwal

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

Ultrasound modulated bioluminescence tomography (UMBLT) is an imaging method which can be formulated as a hybrid inverse source problem. In the regime where light propagation is modeled by a radiative transfer equation, previous approaches…

Analysis of PDEs · Mathematics 2020-08-14 Francis Chung , Tianyu Yang , Yang Yang

Cryopreservation via vitrification requires loading cryoprotective cocktails. Insufficient loading may lead to freezing, precluding successful recovery; overloading is toxic. Yet, existing in situ measurements of cryoprotectant permeation…

The polarized fluorescence emission of organic fluorophores has been extensively studied in photonics and is increasingly exploited in single molecule scale bio-imaging. Expanding the polarization properties of compact molecular assemblies…

Mesoscale and Nanoscale Physics · Physics 2021-01-06 A. Badon , C. Allard , F. Fossard , A. Loiseau , L. Cognet , E. Flahaut , N. Izard , R. Martel , E. Gaufrès

We introduce Modulated Orientation Sensitive Terahertz Spectroscopy (MOSTS) that can both increase contrast between adjacent modes and remove the broad band librational background to allow mode identification for large macro molecules such…

Biomolecules · Quantitative Biology 2012-10-30 Rohit Singh , Deepu Koshy George , Andrea Markelz

We propose a method to reconstruct the density of a luminescent source in a highly-scattering medium from ultrasound modulated optical measurements. Our approach is based on the solution to a hybrid inverse source problem for the diffusion…

Optics · Physics 2015-06-17 Guillaume Bal , John C Schotland

The emerging technique of mid-infrared optical coherence tomography (MIR-OCT) takes advantage of the reduced scattering of MIR light in various materials and devices, enabling tomographic imaging at deeper penetration depths. Because of…