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Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Incoherently illuminated or luminescent objects give rise to a low-contrast speckle-like pattern when observed through a thin diffusive medium, as such a medium effectively convolves their shape with a speckle-like point spread function…

A new and simple method of blood bilirubin detection through light reflection from skin is developed. The basic improvement over the existing methods is in the design of the light emitter and detector geometry which facilitates a two-stream…

Optics · Physics 2007-05-23 Bedrettin Subasilar

The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response…

Image and Video Processing · Electrical Eng. & Systems 2021-08-18 Ganna Platonova , Dalibor Stys , Pavel Soucek , Kirill Lonhus , Jan Valenta , Renata Rychtarikova

This work demonstrates a multi-lens microscopic imaging system that overlaps multiple independent fields of view on a single sensor for high-efficiency automated specimen analysis. Automatic detection, classification and counting of various…

Computer Vision and Pattern Recognition · Computer Science 2022-01-19 Xing Yao , Vinayak Pathak , Haoran Xi , Amey Chaware , Colin Cooke , Kanghyun Kim , Shiqi Xu , Yuting Li , Timothy Dunn , Pavan Chandra Konda , Kevin C. Zhou , Roarke Horstmeyer

Achieving fast, large-scale volumetric imaging with micrometer resolution has been a persistent challenge in the field of biological microscopy. To address this challenge, we report an augmented version of light field microscopy,…

Optics · Physics 2024-10-11 Aymerick Bazin , Amaury Badon

The intricate nature of the brain necessitates the application of advanced probing techniques to comprehensively study and understand its working mechanisms. Neurophotonics offers minimally invasive methods to probe the brain using optics…

This paper proposes a non-computational method of counteracting the effect of image degradation introduced by the diffraction phenomenon in lensless microscopy. All the optical images (whether focused by lenses or not) are diffraction…

Optics · Physics 2021-04-08 Sanjeev Kumar , Manjunatha Mahadevappa , Pranab Kumar Dutta

The use of photonic crystals, which were fabricated on the basis of synthetic opals, as substrates for the luminescence microscopy of biological objects has been shown. The spatial distributions of the photoluminescence by DNA clusters…

We present an optical metrology system for characterization of topography of micro/nano-structures on a surface or embedded in a semi-transparent material. Based on the principles of scatterometry, where the intensity of scattered light is…

Instrumentation and Detectors · Physics 2016-01-15 Morten Hannibal Madsen , Poul-Erik Hansen

Imaging Raman spectroscopy can be used to identify cancerous tissue. Traditionally, a step-by-step scanning of the sample is applied to generate a Raman image, which, however, is too slow for routine examination of patients. By transferring…

Instrumentation and Methods for Astrophysics · Physics 2018-10-09 Elmar Schmälzlin , Benito Moralejo , Ingo Gersonde , Johannes Schleusener , Maxim E. Darvin , Gisela Thiede , Martin M. Roth

Diffraction-limited imaging through complex scattering media is a long sought after goal with important applications in biomedical research. In recent years, high resolution wavefront-shaping has emerged as a powerful approach to generate a…

Optics · Physics 2014-10-17 Ori Katz , Eran Small , Yefeng Guan , Yaron Silberberg

Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint…

We propose and numerically demonstrate a technique for subwavelength imaging based on a metal-dielectric multilayer hyperlens designed in such a way that only the large-wavevector waves are transmitted while all propagating waves from the…

Optics · Physics 2015-10-28 Taavi Repän , Andrei V. Lavrinenko , Sergei V. Zhukovsky

This paper proposes a novel automatic classification framework for the recognition of five types of white blood cells. Segmenting complete white blood cells from blood smears images and extracting advantageous features from them remain…

Computer Vision and Pattern Recognition · Computer Science 2020-09-07 Na Dong , Meng-die Zhai , Jian-fang Chang , Chun-ho Wu

Accurately classifying white blood cells from microscopic images is essential to identify several illnesses and conditions in medical diagnostics. Many deep learning technologies are being employed to quickly and automatically classify…

Image and Video Processing · Electrical Eng. & Systems 2025-08-07 Tatwadarshi P. Nagarhalli , Shruti S. Pawar , Soham A. Dahanukar , Uday Aswalekar , Ashwini M. Save , Sanket D. Patil

Imaging through dense scattering media - such as biological tissue, fog, and smoke - has applications in the medical and robotics fields. We propose a new framework using automatic differentiation for All Photons Imaging through homogeneous…

Image and Video Processing · Electrical Eng. & Systems 2020-06-04 Tomohiro Maeda , Ankit Ranjan , Ramesh Raskar

Tissue oxygenation and perfusion can be an indicator for organ viability during minimally invasive surgery, for example allowing real-time assessment of tissue perfusion and oxygen saturation. Multispectral imaging is an optical modality…

Computer Vision and Pattern Recognition · Computer Science 2017-06-23 Geoffrey Jones , Neil T Clancy , Xiaofei Du , Maria Robu , Simon Arridge , Daniel S Elson , Danail Stoyanov

We demonstrate sub-wavelength sectioning on biological samples with a conventional confocal microscope. This optical sectioning is achieved by the phenomenon of supercritical angle fuorescence, wherein only a fluorophore next to the…

We develop a means for speckle-based phase imaging of the projected thickness of a single-material object, under the assumption of illumination by spatially random time-independent x-ray speckles. These speckles are generated by passing x…

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