Related papers: Imaging blood cells through scattering biological …
Optical time-reversal techniques are being actively developed to focus light through or inside opaque scattering media. When applied to biological tissue, these techniques promise to revolutionize biophotonics by enabling deep-tissue…
High-resolution optical microscopy has transformed biological imaging, yet its resolution and contrast deteriorate with depth due to multiple light scattering. Conventional correction strategies typically approximate the medium as one or a…
Wavefront shaping is a powerful method to refocus light through a scattering medium. Its application to large spectral bandwidths or multiple wavelengths refocusing for nonlinear bio-imaging in-depth is however limited by spectral…
We demonstrate a high-speed method to image objects through a thin scattering medium and around a corner. The method employs a reference object of known shape to retrieve the speckle-like point spread function of the scatterer. We extract…
When light travels through scattering media, speckles (spatially random distribution of fluctuated intensities) are formed due to the interference of light travelling along different optical paths, preventing the perception of structure,…
A number of questions in systems biology such as understanding how dynamics of neuronal networks are related to brain function require the ability to capture the functional dynamics of large cellular populations at high speed. Recently,…
Recently introduced angular-memory-effect based techniques enable non-invasive imaging of objects hidden behind thin scattering layers. However, both the speckle-correlation and the bispectrum analysis are based on the statistical average…
Inspection of tissues using a light microscope is the primary method of diagnosing many diseases, notably cancer. Highly multiplexed tissue imaging builds on this foundation, enabling the collection of up to 60 channels of molecular…
Scattering, especially multiple scattering, is a well known problem in imaging, ranging from astronomy to medicine. In particular it is often desirable to be able to perform non-invasive imaging through turbid and/or opaque media. Many…
By their very nature microscopy images of cells and tissues consist of a limited number of object types or components. In contrast to most natural scenes, the composition is known a priori. Decomposing biological images into semantically…
Current biological and medical research is aimed at obtaining a detailed spatiotemporal map of a live cell's interior to describe and predict cell's physiological state. We present here an algorithm for complete 3-D modelling of cellular…
High-resolution optical microscopy suffers from a low contrast in scattering media where a multiply scattered wave obscures a ballistic wave used for image formation. To extend the imaging depth, various gating operations - confocal,…
In this chapter a general mathematical model of Optical Coherence Tomography (OCT) is presented on the basis of the electromagnetic theory. OCT produces high resolution images of the inner structure of biological tissues. Images are…
Due to the complex of mixed spectral point spread function within memory effect range, it is unreliable and slow to use speckle correlation technology for non-invasive imaging through scattering medium under broadband illumination. The…
Accurate segmentation of live cell images has broad applications in clinical and research contexts. Deep learning methods have been able to perform cell segmentations with high accuracy; however developing machine learning models to do this…
Fluorescence microscopy is widely used in biological imaging, however scattering from tissues strongly limits its applicability to a shallow depth. In this work we adapt a methodology inspired from stellar speckle interferometry, and…
Aberrations limit scanning fluorescence microscopy when imaging in scattering materials such as biological tissue. Model-based approaches for adaptive optics take advantage of a computational model of the optical setup. Such models can be…
Scattering can rapidly degrade our ability to form an optical image, to the point where only speckle-like patterns can be measured. Truly non-invasive imaging through a strongly scattering obstacle is difficult, and usually reliant on a…
As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and…
We combine confocal imaging, microfluidics and image analysis to record 3D-images of cells in flow. This enables us to recover the full 3D representation of several hundred living cells per minute. Whereas 3D confocal imaging has thus far…