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We describe a simple and robust approach for characterizing the shift-variant pupil aberrations of wide field-of-view microscopy systems. We derive the location-dependent pupil transfer functions by first capturing multiple intensity images…

Optics · Physics 2016-12-21 Guoan Zheng , Xiaoze Ou , Roarke Horstmeyer , Changhuei Yang

Many adaptive optics systems operate by measuring the distortion of the wavefront in one wavelength range and performing the scientific observations in a second, different wavelength range. One common technique is to measure wavefront…

Astrophysics · Physics 2009-11-07 Henry G. Roe

Adaptive optics in combination with multi-photon techniques is a powerful approach to image deep into a specimen. Remarkably, virtually all adaptive optics schemes today rely on wavefront modulators which are reflective, diffractive, or…

All optical systems, which involve the collimation of a reflected, transmitted or scattered wave subsequent to tight focusing, are subject to two kinds of deviations. One is the wavefront curvature due to inaccurate focal placement of the…

Optics · Physics 2024-06-10 Nitish Kumar , Anirban Debnath , Nirmal K. Viswanathan

Optical aberrations prevent telescopes from reaching their theoretical diffraction limit. Once estimated, these aberrations can be compensated for using deformable mirrors in a closed loop. Focal plane wavefront sensing enables the…

Depth of field is an important factor of imaging systems that highly affects the quality of the acquired spatial information. Extended depth of field (EDoF) imaging is a challenging ill-posed problem and has been extensively addressed in…

Image and Video Processing · Electrical Eng. & Systems 2020-05-27 Ugur Akpinar , Erdem Sahin , Monjurul Meem , Rajesh Menon , Atanas Gotchev

Imaging techniques such as functional near-infrared spectroscopy (fNIRS) and diffuse optical tomography (DOT) achieve deep, non-invasive sensing in turbid media, but they are constrained by the photon budget. Wavefront shaping (WFS) can…

Optics · Physics 2026-04-21 Pablo Jara , Arthur Goetschy , Hui Cao , Alexey Yamilov

The detection and characterisation of extra-solar planets is a major theme driving modern astronomy, with the vast majority of such measurements being achieved by Doppler radial-velocity and transit observations. Another technique -- direct…

Instrumentation and Methods for Astrophysics · Physics 2015-06-19 Barnaby Norris , Nick Cvetojevic , Simon Gross , Nemanja Jovanovic , Paul N. Stewart , Ned Charles , Jon S. Lawrence , Michael J. Withford , Peter Tuthill

Focal plane wavefront sensing and control is a critical approach to reducing non-common path errors between the a conventional astronomical adaptive optics (AO) wavefront sensor (WFS) detector and science camera. However, in addition to…

Instrumentation and Methods for Astrophysics · Physics 2023-03-01 Benjamin L. Gerard , Daren Dillon , Sylvain Cetre , Rebecca Jensen-Clem

Wavefront estimation using in-focus image data is critical to many applications. This data is invariant to a sign flip with complex conjugation of the complex amplitude in the pupil, making for a non-unique solution. Information from an…

Instrumentation and Methods for Astrophysics · Physics 2016-07-08 Alexandra Greenbaum , Anand Sivaramakrishnan

Fourier ptychography (FP) is a powerful light-based synthetic aperture imaging technique that allows one to reconstruct a high-resolution, wide field-of-view image by computationally integrating a diverse collection of low-resolution,…

Image and Video Processing · Electrical Eng. & Systems 2025-07-21 Matthew A. Chan , Casey J. Pellizzari , Christopher A. Metzler

High-contrast imaging systems using active control with adaptive optics (AO) are often limited by non-common path (NCP) aberrations that are seen only at the final science image. AO systems employing focal-plane wavefront sensors (FP-WFSs)…

Instrumentation and Methods for Astrophysics · Physics 2026-01-22 Aditya R. Sengupta , Lisa A. Poyneer , Benjamin L. Gerard , Rebecca Jensen-Clem , Aaron J. Lemmer

We demonstrate a three-dimensional (3D) optical diffraction tomographic technique with optimal frequency combination (OFC-ODT) for the 3D quantitative phase imaging of unlabeled specimens. Three sets of through-focus intensity images are…

Optics · Physics 2018-03-06 Jiaji Li , Qian Chen Jiasong Sun , Jialin Zhang , Junyi Ding , Chao Zuo

Optical coherence tomography (OCT) is a prevalent, interferometric, high-resolution imaging method with broad biomedical applications. Nonetheless, OCT images suffer from an artifact, called speckle which degrades the image quality. Digital…

The imaging performance of an optical microscope can be degraded by sample-induced aberrations. A general strategy to undo the effect of these aberrations is to apply wavefront correction with a deformable mirror (DM). In most cases, the DM…

Optics · Physics 2015-06-23 Jerome Mertz , Hari Paudel , Thomas G. Bifano

Complex field imaging, which captures both the amplitude and phase information of input optical fields or objects, can offer rich structural insights into samples, such as their absorption and refractive index distributions. However,…

Optics · Physics 2024-05-30 Jingxi Li , Yuhang Li , Tianyi Gan , Che-Yung Shen , Mona Jarrahi , Aydogan Ozcan

With its high sensitivity, the Pyramid wavefront sensor (PyWFS) is becoming an advantageous sensor for astronomical adaptive optics (AO) systems. However, this sensor exhibits significant non-linear behaviours leading to challenging AO…

Instrumentation and Methods for Astrophysics · Physics 2021-05-12 Vincent Chambouleyron , Olivier Fauvarque , Jean-François Sauvage , Benoît Neichel , Thierry Fusco

The basic outline of a pupil plane WaveFront Sensor is reviewed taking into account that the source to be sensed could be different from an unresolved source, i.e. it is extended, and that it could deploy also in a 3D fashion, enough to…

Digital cameras and displays utilise picture elements (pixels) that perform a single function: detecting or emitting light intensity. To exploit the full information content of electromagnetic waves, more advanced elements are required.…

Light microscopy enables multifunctional imaging of biological specimens at unprecedented depths and resolutions. However, the performance of all optical methods degrades with the imaging depth due to sample-induced aberrations. Methods of…

Optics · Physics 2019-02-06 Lyubov V. Amitonova