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Optical coherence tomography (OCT) microscope (OCM) uses a high-numerical-aperture objective to achieve cellular-level lateral resolution. However, its practical imaging depth range is limited by the depth of focus (DOF). Although…

We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (0.2 micron) and temporal (50 psec) resolution and…

Quantitative Methods · Quantitative Biology 2009-11-10 R. V. Krishnan , H. Saitoh , H. Terada , V. E. Centonze , B. Herman

Atomic time scale imaging, opening a new era for studying dynamics in microcosmos, is presently attracting immense research interesting on the global level due to its powerful ability. On the atom level, physics, chemistry, and biology are…

Optics · Physics 2023-10-19 Jingzhen Li , Yi Cai , Xuanke Zeng , Xiaowei Lu , Qifan Zhu , Yongle Zhu

Atomic resolution imaging in transmission electron microscopy (TEM) and scanning TEM (STEM) of light elements in electron-transparent materials has long been a challenge. Biomolecular materials, for example, are rapidly altered when…

Instrumentation and Detectors · Physics 2018-12-05 Fehmi S. Yasin , Tyler R. Harvey , Jordan J. Chess , Jordan S. Pierce , Colin Ophus , Peter Ercius , Benjamin J. McMorran

Fast and sensitive detector arrays enable image scanning microscopy (ISM), overcoming the trade-off between spatial resolution and signal-to-noise ratio (SNR) typical of confocal microscopy. However, current ISM approaches cannot provide…

Occlusions between consecutive frames have long posed a significant challenge in optical flow estimation. The inherent ambiguity introduced by occlusions directly violates the brightness constancy constraint and considerably hinders…

Computer Vision and Pattern Recognition · Computer Science 2023-11-30 Shangkun Sun , Jiaming Liu , Thomas H. Li , Huaxia Li , Guoqing Liu , Wei Gao

Manual operation of microscopes for repetitive tasks in cell biology is a significant bottleneck, consuming invaluable expert time, and introducing human error. Automation is essential, and while Digital Holographic Microscopy (DHM) offers…

Retinal imaging has emerged as a powerful, non-invasive modality for detecting and quantifying biomarkers of systemic diseases-ranging from diabetes and hypertension to Alzheimer's disease and cardiovascular disorders but current insights…

Image and Video Processing · Electrical Eng. & Systems 2025-05-28 Tariq M Khan , Toufique Ahmed Soomro , Imran Razzak

With the continuing advances in scientific instrumentation, scanning microscopes are now able to image physical systems with up to sub-atomic-level spatial resolutions and sub-picosecond time resolutions. Commensurately, they are generating…

This paper presents a new algorithm, Accelerated Wirtinger Flow (AWF), for ptychographic image reconstruction from phaseless diffraction pattern measurements. AWF is based on combining Nesterov's acceleration approach with Wirtinger…

Image and Video Processing · Electrical Eng. & Systems 2018-06-29 Rui Xu , Mahdi Soltanolkotabi , Justin P. Haldar , Walter Unglaub , Joshua Zusman , Anthony F. J. Levi , Richard M. Leahy

Time-domain sampling of arbitrary electric fields with sub-cycle resolution enables a complete time-frequency analysis of a system's response to electromagnetic illumination. This provides access to dynamic information that is not provided…

Orbital angular momentum (OAM) detection underpins almost all aspects of vortex beams' advances such as communication and quantum analogy. Conventional schemes are frustrated by low speed, complicated system, limited detection range. Here,…

Emerging Technologies · Computer Science 2022-09-07 Hao Wang , Ziyu Zhan , Futai Hu , Yuan Meng , Zeqi Liu , Xing Fu , Qiang Liu

Operando/in-situ liquid cell transmission electron microscopy (LCTEM) allows for real time imaging of dynamic nanoscale liquid-based processes. However, due to the thick liquid cell of traditional LCTEM holders and thus scattering of the…

Optically pumped magnetometers (OPMs) have emerged as a powerful technique for high-resolution magnetic field imaging. However, achieving sub-millimeter spatial resolution at sub-picotesla sensitivities ($\mathrm{< 1\,pT/\sqrt{Hz}}$)…

In the effort to aid cytologic diagnostics by establishing automatic single cell screening using high throughput digital holographic microscopy for clinical studies thousands of images and millions of cells are captured. The bottleneck lies…

Image and Video Processing · Electrical Eng. & Systems 2024-10-28 Julia Sistermanns , Ellen Emken , Gregor Weirich , Oliver Hayden , Wolfgang Utschick

We introduce Time-Conditioned Contraction Matching (TCCM), a novel method for semi-supervised anomaly detection in tabular data. TCCM is inspired by flow matching, a recent generative modeling framework that learns velocity fields between…

Machine Learning · Computer Science 2025-10-22 Zhong Li , Qi Huang , Yuxuan Zhu , Lincen Yang , Mohammad Mohammadi Amiri , Niki van Stein , Matthijs van Leeuwen

Scanning ion conductance microscopy (SICM) can image the surface topography of specimens in ionic solutions without mechanical probe--sample contact. This unique capability is advantageous for imaging fragile biological samples but its…

Applied Physics · Physics 2019-12-25 Shinji Watanabe , Satoko Kitazawa , Linhao Sun , Noriyuki Kodera , Toshio Ando

Motivation: Cellular Electron CryoTomography (CECT) is an emerging 3D imaging technique that visualizes subcellular organization of single cells at submolecular resolution and in near-native state. CECT captures large numbers of…

Quantitative Methods · Quantitative Biology 2018-05-16 Yixiu Zhao , Xiangrui Zeng , Qiang Guo , Min Xu

Factors that contribute to the rapid increase in power dissipation as a function of input bandwidth in high speed electronic Analog-to-Digital Converters (ADCs) are discussed. We find that the figure of merit (FOM), defined as the energy…

Instrumentation and Detectors · Physics 2009-01-20 Shalabh Gupta , George C. Valley , Robert H. Walden , Bahram Jalali

The brightfield microscope is instrumental in the visual examination of both biological and physical samples at sub-millimeter scales. One key clinical application has been in cancer histopathology, where the microscopic assessment of the…

Computer Vision and Pattern Recognition · Computer Science 2020-06-03 Po-Hsuan Cameron Chen , Krishna Gadepalli , Robert MacDonald , Yun Liu , Kunal Nagpal , Timo Kohlberger , Jeffrey Dean , Greg S. Corrado , Jason D. Hipp , Martin C. Stumpe