Related papers: Full Field Supercritical Angle Fluorescence Micros…
Two-photon excited fluorescence (TPEF) microscopy and fluorescence lifetime imaging (FLIM) are powerful imaging techniques in bio-molecular science. The need for elaborate light sources for TPEF and speed limitations for FLIM, however,…
Exposure to intense illumination light is an unavoidable consequence of fluorescence microscopy, and poses a risk to the health of the sample in every live-cell fluorescence microscopy experiment. Furthermore, the possible side-effects of…
We introduce an absorption imaging technique for ultracold gases that suppresses interference fringes and coherence-induced artifacts by reducing the transverse spatial coherence of the imaging light. The method preserves the narrow…
Mid-infrared spectroscopy probes molecular vibrations to identify chemical species and functional groups. Therefore, mid-infrared hyperspectral imaging is one of the most powerful and promising candidates for chemical imaging using optical…
Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…
Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several…
I propose a superoscillation measurement method for subdiffraction incoherent optical sources, with potential applications in astronomy, remote sensing, fluorescence microscopy, and spectroscopy. The proposal, based on coherent optical…
Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…
Imaging assays of cellular function, especially those using fluorescent stains, are ubiquitous in the biological and medical sciences. Despite advances in computer vision, such images are often analyzed using only manual or rudimentary…
There is widespread interest in estimating the fluorescence properties of natural materials in an image. However, the separation between reflected and fluoresced components is difficult, because it is impossible to distinguish reflected and…
In vivo fluorescence imaging in the second near-infrared window (1.0-1.7 microns) can afford deep tissue penetration and high spatial resolution, owing to the reduced scattering of long-wavelength photons. Here, we synthesize a series of…
We present simulations of an efficient high numerical aperture solid immersion metalens concept for fluorescence microscopy. The technique exploits the preferential emission of interfacial dipoles into a high refractive index substrate…
In this work, we present a method for absolute measurement of air fluorescence yield based on high resolution optical emission spectroscopy. The absolute measurement of the air fluorescence yield is feasible using the Cherenkov light,…
We report on the 3-dimensional imaging of biological cells including living neurons by a recently developed fibered interferometric Scanning Optical Microscope. The topography and surface structure of mouse fibroblasts and hippocampal…
Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-scanning. This constitutes…
Improving the resolution of fluorescence microscopy beyond the diffraction limit can be achievedby acquiring and processing multiple images of the sample under different illumination conditions.One of the simplest techniques, Random…
We measured images and Fourier images of fluorescence for 0.11- and 0.22-$\mu$m-diameter dye-doped polystyrene micro-sphere beads on a solid immersion lens, and experimentally verified strongly-angle-dependent fluorescence intensities due…
Single-shot fluorescence imaging techniques have gained increasing interest in recent years due to their ability to rapidly capture complex biological data without the need for extensive scanning. In this letter, we introduce polarized…
We present a novel method for real-time monitoring and kinetic analysis of fluorescence in situ hybridization (FISH). We implement the method using a vertical microfluidic probe containing a microstructure designed for rapid switching…
Long baseline diffraction-limited optical aperture synthesis technology by interferometry plays an important role in scientific study and practical application. In contrast to amplitude (phase) interferometry, intensity interferometry --…