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We demonstrate that a deep neural network can significantly improve optical microscopy, enhancing its spatial resolution over a large field-of-view and depth-of-field. After its training, the only input to this network is an image acquired…

Machine Learning · Computer Science 2017-11-21 Yair Rivenson , Zoltan Gorocs , Harun Gunaydin , Yibo Zhang , Hongda Wang , Aydogan Ozcan

Microscopy is routinely used to image biological structures of interest. Due to imaging constraints, acquired images, also called as micrographs, are typically low-SNR and contain noise. Over the last few years, regression-based tasks like…

Image and Video Processing · Electrical Eng. & Systems 2024-10-16 Ashesh Ashesh , Joran Deschamps , Florian Jug

Conventional LIDAR systems require hundreds or thousands of photon detections to form accurate depth and reflectivity images. Recent photon-efficient computational imaging methods are remarkably effective with only 1.0 to 3.0 detected…

Applications · Statistics 2019-11-13 Joshua Rapp , Vivek K Goyal

Phase is a fundamental resource for optical imaging but cannot be directly observed with intensity measurements. The existing methods to quantify a phase distribution rely on complex devices and structures. Here we experimentally…

Optics · Physics 2020-03-25 Tengfeng Zhu , Junyi Huang , Zhichao Ruan

The dark-field signal uncovers details beyond conventional X-ray attenuation contrast, which is especially valuable for material sciences. In particular, dark-field techniques are able to reveal structures beyond the spatial resolution of a…

We present a novel approach for benchmarking and validating quantitative phase tomography (QPT) systems using three-dimensional microphantoms. These microphantoms, crafted from biological and imaging data, replicate the optical and…

Label-free imaging of rapidly moving, sub-diffraction sized structures has important applications in both biology and material science, as it removes the limitations associated with fluorescence tagging. However, unlabeled nanoscale…

Image-based lighting is a widely used technique to reproduce shading under real-world lighting conditions, especially in real-time rendering applications. A particularly challenging scenario involves materials exhibiting a sparkling or…

Graphics · Computer Science 2025-07-04 Tom Kneiphof , Reinhard Klein

Imaging depth of optical microscopy has been fundamentally limited to millimeter or sub-millimeter due to light scattering. X-ray microscopy can resolve spatial details of few microns deeply inside a sample but the contrast resolution is…

Biological Physics · Physics 2015-06-17 Wenxiang Cong , Fenglin Liu , Chao Wang , Ge Wang

Imaging specimens over large scales and with a sub-micron resolution is instrumental to biomedical research. Yet, the number of pixels to form such an image usually exceeds the number of pixels provided by conventional cameras. While most…

Instrumentation and Detectors · Physics 2019-12-03 Gaelle Recher , Pierre Nassoy , Amaury Badon

Due to its specificity, fluorescence microscopy (FM) has become a quintessential imaging tool in cell biology. However, photobleaching, phototoxicity, and related artifacts continue to limit FM's utility. Recently, it has been shown that…

Multidimensional imaging techniques provide powerful ways to examine various kinds of scientific questions. The routinely produced datasets in the terabyte-range, however, can hardly be analyzed manually and require an extensive use of…

Computer Vision and Pattern Recognition · Computer Science 2016-08-31 Johannes Stegmaier

We report a novel lensless on-chip microscopy platform based on near-field blind ptychographic modulation. In this platform, we place a thin diffuser in between the object and the image sensor for light wave modulation. By blindly scanning…

Image and Video Processing · Electrical Eng. & Systems 2020-02-13 Shaowei Jiang , Jiakai Zhu , Pengming Song , Chengfei Guo , Zichao Bian , Ruihai Wang , Yikun Huang , Shiyao Wang , He Zhang , Guoan Zheng

By their very nature microscopy images of cells and tissues consist of a limited number of object types or components. In contrast to most natural scenes, the composition is known a priori. Decomposing biological images into semantically…

Computer Vision and Pattern Recognition · Computer Science 2019-09-04 Avelino Javer , Jens Rittscher

Several Scientific and engineering applications require merging of sampled images for complex perception development. In most cases, for such requirements, images are merged at intensity level. Even though it gives fairly good perception of…

Computer Vision and Pattern Recognition · Computer Science 2014-08-01 T. R. Gopalakrishnan Nair , Richa Sharma

When one captures images in low-light conditions, the images often suffer from low visibility. This poor quality may significantly degrade the performance of many computer vision and multimedia algorithms that are primarily designed for…

Computer Vision and Pattern Recognition · Computer Science 2016-07-26 Xiaojie Guo

The conversion of phase variations in an optical wavefield into intensity information is of fundamental importance for optical imaging technology including microscopy of biological cells. While conventional approaches to phase-imaging…

Optics · Physics 2022-09-07 Lukas Wesemann , Jon Rickett , Timothy J. Davis , Ann Roberts

Quantitative phase imaging (QPI) is important in many applications such as microscopy and crystallography. To quantitatively reveal phase information, people could either employ interference to map phase distribution into intensity fringes,…

Optics · Physics 2020-11-11 Xianye Li , Yafei sun , Yikang He , Xun Li , Baoqing Sun

A new algorithmic framework is presented for holographic phase retrieval via maximum likelihood optimization, which allows for practical and robust image reconstruction. This framework is especially well-suited for holographic coherent…

Image and Video Processing · Electrical Eng. & Systems 2022-02-18 David A. Barmherzig , Ju Sun

Interferometric scattering microscopy is a powerful technique that enables various applications, such as mass photometry and particle tracking. Here we present a numerical toolbox to simulate images obtained in interferometric scattering,…