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Super-resolution microscopy, or nanoscopy, enables the use of fluorescent-based molecular localization tools to study molecular structure at the nanoscale level in the intact cell, bridging the mesoscale gap to classical structural biology…

Subcellular Processes · Quantitative Biology 2024-05-28 Ivan R. Nabi , Ben Cardoen , Ismail M. Khater , Guang Gao , Timothy H. Wong , Ghassan Hamarneh

As demonstrated by Boto et al. [Phys. Rev. Lett. 85, 2733 (2000)], quantum lithography offers an increase in resolution below the diffraction limit. Here, we generalize this procedure in order to create patterns in one and two dimensions.…

Light-field microscopy represents a promising solution for microscopic volumetric imaging, thanks to its capability to encode information on multiple planes in a single acquisition. This is achieved through its peculiar simultaneous capture…

Live cell imaging using metallic nanoparticles as tags is an emerging technique to visualize long and highly dynamic processes due to the lack of photobleaching and high photon rate. However, the lack of excited states as compared to…

Mesoscale and Nanoscale Physics · Physics 2011-01-12 H. E. Grecco , O. E. Martínez

We introduce MINFLUX, a concept for localizing photon emitters in space. By probing the emitter with a local intensity minimum of excitation light, MINFLUX minimizes the fluorescence photons needed for high localization precision. A 22-fold…

Recent advances in optical technology have significantly enhanced the resolution of imaging of living cells, achieving nanometer-scale precision. However, the crowded three-dimensional environment within cells presents a challenge for…

Optics · Physics 2024-11-20 Vahid Nasirimarekani , Zuzana Ditte , Eberhard Bodenschatz

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

To overcome the limit of diffraction while achieving the superresolution technique, solid immersion lenses are the key optical elements for data storage and nanophotonics applications. Recent demonstrations have shown how different…

Optics · Physics 2026-02-24 Subhankar Roy , Jianping Hu , M Ummal Momeen

We present a scheme for nanoscopic imaging of a quantum mechanical two-level system using an optical probe in the far-field. Existing super-resolution schemes require more than two-levels and depend on an incoherent response to the lasers.…

Mesoscale and Nanoscale Physics · Physics 2017-01-09 Timo Kaldewey , Andreas V. Kuhlmann , Sascha R. Valentin , Arne Ludwig , Andreas D. Wieck , Richard J. Warburton

The proliferation of microscopy methods for live-cell imaging offers many new possibilities for users but can also be challenging to navigate. We focus here on computational methods that promise to boost live-cell fluorescence microscopy,…

Quantitative Methods · Quantitative Biology 2024-01-04 Hari Shroff , Ilaria Testa , Florian Jug , Suliana Manley

Coherent diffractive imaging is unique as the only route for achieving diffraction-limited spatial resolution in the extreme ultraviolet and X-ray regions, limited only by the wavelength of the light. Recently, advances in coherent short…

We propose a quantum-assisted reconstruction framework for high-resolution tomographic imaging that significantly reduces both qubit requirements and radiation exposure. Conventional quantum reconstruction methods require solving QUBO…

Quantum Physics · Physics 2025-04-30 Hyunju Lee , Kyungtaek Jun

The wave-particle duality of light introduces two fundamental problems to imaging, namely, the diffraction limit and the photon shot noise. Quantum information theory can tackle them both in one holistic formalism: model the light as a…

Quantum Physics · Physics 2020-04-10 Mankei Tsang

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently…

Biological Physics · Physics 2017-05-02 Shwetadwip Chowdhury , Will J. Eldridge , Adam Wax , Joseph A. Izatt

Modern imaging technologies are widely based on classical principles of light or electromagnetic wave propagation. They can be remarkably sophisticated, with recent successes ranging from single molecule microscopy to imaging far-distant…

Recent advances in propagation-based phase-contrast imaging, such as hierarchical imaging, have enabled the visualization of internal structures in large biological specimens and material samples. However, modulation-based techniques, which…

Quantum metrology exploits quantum resources to enhance measurement precision beyond the classical limit. Conventional protocols normally rely on the preparation of delicate quantum states to acquire these resources, posing a major…

Quantum Physics · Physics 2026-03-31 Yuan Liu , Ke-Mi Xu , Hong-Bo Sun , Linhan Lin

Superresolution fluorescence microscopy techniques beat the diffraction limit, enabling ultra-high resolution imaging in biological physics and nanoscience. In all cases that have been studied experimentally, the resolution scales inversely…

Optics · Physics 2012-10-10 Alexander Small

Polarimetric imaging, a technique that captures the invisible polarization-related properties of given materials, has broad applications from fundamental physics to advanced fields such as target recognition, stress detection, biomedical…

Quantum metrology has been shown to surpass classical limits of correlation, resolution, and sensitivity. It has been introduced to interferometric Radar schemes, with intriguing preliminary results. Even quantum-inspired detection of…

Quantum Physics · Physics 2024-01-11 Leonid Vidro , Liran Shirizly , Naftali Kirsh , Nadav Katz , Hagai S. Eisenberg