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High-throughput mRNA sequencing (RNA-Seq) is widely used for transcript quantification of gene isoforms. Since RNA-Seq data alone is often not sufficient to accurately identify the read origins from the isoforms for quantification, we…

Computational Engineering, Finance, and Science · Computer Science 2015-12-31 Wei Zhang , Jae-Woong Chang , Lilong Lin , Kay Minn , Baolin Wu , Jeremy Chien , Jeongsik Yong , Hui Zheng , Rui Kuang

Cell populations are never truly homogeneous; individual cells exist in biochemical states that define functional differences between them. New technology based on microfluidic arrays combined with multiplexed quantitative polymerase chain…

Single-cell RNA sequencing (scRNA-seq) technology enables systematic delineation of cellular states and interactions, providing crucial insights into cellular heterogeneity. Building on this potential, numerous computational methods have…

Genomics · Quantitative Biology 2025-11-11 Ping Xu , Zaitian Wang , Zhirui Wang , Pengjiang Li , Ran Zhang , Gaoyang Li , Hanyu Xie , Jiajia Wang , Yuanchun Zhou , Pengfei Wang

Gene expression analysis is a critical method for cancer classification, enabling precise diagnoses through the identification of unique molecular signatures associated with various tumors. Identifying cancer-specific genes from gene…

Quantitative Methods · Quantitative Biology 2024-10-11 Farzana Tabassum , Sabrina Islam , Siana Rizwan , Masrur Sobhan , Tasnim Ahmed , Sabbir Ahmed , Tareque Mohmud Chowdhury

We have developed a statistical method named IsoDOT to assess differential isoform expression (DIE) and differential isoform usage (DIU) using RNA-seq data. Here isoform usage refers to relative isoform expression given the total expression…

Using methods of differential evolution (DE,) we determined the coronal elemental abundances and the differential emission measure (DEM) distributions for the plasma flaring on 2003 January 21. The analyses have been based on RESIK X-ray…

Solar and Stellar Astrophysics · Physics 2022-03-09 Anna Kepa , Barbara Sylwester , Marek Siarkowski , Janusz Sylwester

One of the most popular tools for large scale gene expression studies are high-density oligonucleotide (GeneChip(R)) arrays. These currently have 16-20 small probe cells (``features'') for evaluating the transcript abundance of each gene.…

Biological Physics · Physics 2007-05-23 Felix Naef , Daniel A. Lim , Nila Patil , Marcelo O. Magnasco

One of the most common analysis tasks in genomic research is to identify genes that are differentially expressed (DE) between experimental conditions. Empirical Bayes (EB) statistical tests using moderated genewise variances have been very…

Applications · Statistics 2016-07-28 Belinda Phipson , Stanley Lee , Ian J. Majewski , Warren S. Alexander , Gordon K. Smyth

We consider the problem of predicting gene expressions from DNA sequences. A key challenge of this task is to find the regulatory elements that control gene expressions. Here, we introduce Seq2Exp, a Sequence to Expression network…

Genomics · Quantitative Biology 2025-02-21 Xingyu Su , Haiyang Yu , Degui Zhi , Shuiwang Ji

Single-cell RNA sequencing (scRNA-seq) enables researchers to analyze gene expression at single-cell level. One important task in scRNA-seq data analysis is unsupervised clustering, which helps identify distinct cell types, laying down the…

Genomics · Quantitative Biology 2023-12-29 Weikang Jiang , Jinxian Wang , Jihong Guan , Shuigeng Zhou

We introduce a model of DNA sequence evolution which can account for biases in mutation rates that depend on the identity of the neighboring bases. An analytic solution for this class of non-equilibrium models is developed by adopting…

Biological Physics · Physics 2007-05-23 Peter F. Arndt , Christopher B. Burge , Terence Hwa

Late diagnosis and high costs are key factors that negatively impact the care of cancer patients worldwide. Although the availability of biological markers for the diagnosis of cancer type is increasing, costs and reliability of tests…

Machine Learning · Computer Science 2019-08-20 Sterling Ramroach , Melford John , Ajay Joshi

A transversal topic of my research has been the development and application of computational methods for DNA sequence analysis. The methods I have been developing aim at improving our understanding of the regulation processes happening in…

Genomics · Quantitative Biology 2014-09-24 Valentina Boeva

Current popular methods in literature of RNA sequencing normalisation do not account for gene length when compared across samples, whilst adjusting for count biases in the data. This creates a gap in the normalisation as bigger genes in RNA…

Other Quantitative Biology · Quantitative Biology 2022-09-02 Hilbert Lam Yuen In , Robbe Pincket

Despite great interest in solving RNA secondary structures due to their impact on function, it remains an open problem to determine structure from sequence. Among experimental approaches, a promising candidate is the "chemical modification…

Quantitative Methods · Quantitative Biology 2011-06-30 Sharon Aviran , Julius B. Lucks , Lior Pachter

Single-cell RNA-Sequencing (scRNA-Seq) has undergone major technological advances in recent years, enabling the conception of various organism-level cell atlassing projects. With increasing numbers of datasets being deposited in public…

The single-cell RNA sequencing (scRNA-seq) technology enables researchers to study complex biological systems and diseases with high resolution. The central challenge is synthesizing enough scRNA-seq samples; insufficient samples can impede…

Genomics · Quantitative Biology 2023-12-25 Yixuan Wang , Shuangyin Li , Shimin DI , Lei Chen

Detecting chemical modifications on RNA molecules remains a key challenge in epitranscriptomics. Traditional reverse transcription-based sequencing methods introduce enzyme- and sequence-dependent biases and fragment RNA molecules,…

Background: Single-cell RNA sequencing (scRNA-seq) yields valuable insights about gene expression and gives critical information about complex tissue cellular composition. In the analysis of single-cell RNA sequencing, the annotations of…

Genomics · Quantitative Biology 2023-03-29 Xiaowen Cao , Li Xing , Elham Majd , Hua He , Junhua Gu , Xuekui Zhang

The proliferation of misinformation necessitates robust yet computationally efficient fact verification systems. While current state-of-the-art approaches leverage Large Language Models (LLMs) for generating explanatory rationales, these…

Computation and Language · Computer Science 2025-11-10 Alamgir Munir Qazi , John P. McCrae , Jamal Abdul Nasir