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In a shotgun proteomics experiment, proteins are the most biologically meaningful output. The success of proteomics studies depends on the ability to accurately and efficiently identify proteins. Many methods have been proposed to…

Quantitative Methods · Quantitative Biology 2012-11-30 Chao Yang , Zengyou He , Weichuan Yu

Rationale: In a shotgun proteomics experiment with data-dependent acquisition, real-time analysis of a precursor scan results in selection of a handful of peaks for subsequent isolation, fragmentation and secondary scanning. This peak…

Quantitative Methods · Quantitative Biology 2012-07-26 Benjamin J. Diament , Michael J. MacCoss , William Stafford Noble

Mass spectrometry provides a high-throughput way to identify proteins in biological samples. In a typical experiment, proteins in a sample are first broken into their constituent peptides. The resulting mixture of peptides is then subjected…

Applications · Statistics 2010-11-10 Qunhua Li , Michael J. MacCoss , Matthew Stephens

Often the development of novel functional peptides is not amenable to high throughput or purely computational screening methods. Peptides must be synthesized one at a time in a process that does not generate large amounts of data. One way…

Biomolecules · Quantitative Biology 2020-12-14 Rainier Barrett , Andrew D. White

As in many other scientific domains, we face a fundamental problem when using machine learning to identify proteins from mass spectrometry data: large ground truth datasets mapping inputs to correct outputs are extremely difficult to…

In the genomic era, the identification of gene signatures associated with disease is of significant interest. Such signatures are often used to predict clinical outcomes in new patients and aid clinical decision-making. However, recent…

Methodology · Statistics 2019-03-27 Naim U. Rashid , Quefeng Li , Jen Jen Yeh , Joseph G. Ibrahim

Learning ensembles by bagging can substantially improve the generalization performance of low-bias, high-variance estimators, including those evolved by Genetic Programming (GP). To be efficient, modern GP algorithms for evolving (bagging)…

Neural and Evolutionary Computing · Computer Science 2021-02-08 Marco Virgolin

Shotgun proteomics is a high-throughput technology used to identify unknown proteins in a complex mixture. At the heart of this process is a prediction task, the spectrum identification problem, in which each fragmentation spectrum produced…

Computational Engineering, Finance, and Science · Computer Science 2012-10-19 Ajit P. Singh , John Halloran , Jeff A. Bilmes , Katrin Kirchoff , William S. Noble

This paper proposes a general optimization strategy, which combines results from different optimization or parameter estimation methods to overcome shortcomings of a single method. Shotgun optimization is developed as a framework which…

Computation · Statistics 2017-11-15 Biljana Jonoska Stojkova , David A. Campbell

Bagging is a commonly used ensemble technique in statistics and machine learning to improve the performance of prediction procedures. In this paper, we study the prediction risk of variants of bagged predictors under the proportional…

Statistics Theory · Mathematics 2023-10-26 Pratik Patil , Jin-Hong Du , Arun Kumar Kuchibhotla

In the post-genome era, biologists have sought to measure the complete complement of proteins, termed proteomics. Currently, the most effective method to measure the proteome is with shotgun, or bottom-up, proteomics, in which the proteome…

Genomics · Quantitative Biology 2018-11-30 Jesse G. Meyer

With the insight of variance-bias decomposition, we design a new hybrid bagging-boosting algorithm named SBPMT for classification problems. For the boosting part of SBPMT, we propose a new tree model called Probit Model Tree (PMT) as base…

Machine Learning · Statistics 2023-11-07 Tian Qin , Wei-Min Huang

Background: Significance analysis plays a major role in identifying and ranking genes, transcription factor binding sites, DNA methylation regions, and other high-throughput features for association with disease. We propose a new approach,…

Methodology · Statistics 2017-01-10 Andrew E. Jaffe , John D. Storey , Hongkai Ji , Jeffrey T. Leek

Mass spectrometry is the dominant technology in the field of proteomics, enabling high-throughput analysis of the protein content of complex biological samples. Due to the complexity of the instrumentation and resulting data, sophisticated…

Quantitative measurements produced by mass spectrometry proteomics experiments offer a direct way to explore the role of proteins in molecular mechanisms. However, analysis of such data is challenging due to the large proportion of missing…

Methodology · Statistics 2025-01-22 Haeun Moon , Jin-Hong Du , Jing Lei , Kathryn Roeder

Perturb and Combine (P&C) group of methods generate multiple versions of the predictor by perturbing the training set or construction and then combining them into a single predictor (Breiman, 1996b). The motive is to improve the accuracy in…

Machine Learning · Computer Science 2016-10-05 Harsh Nisar , Bhanu Pratap Singh Rawat

The problem of combining individual forecasters to produce a forecaster with improved performance is considered. The connections between probability elicitation and classification are used to pose the combining forecaster problem as that of…

Methodology · Statistics 2017-07-11 Hamed Masnadi-Shirazi

Sequence discriminative training is a great tool to improve the performance of an automatic speech recognition system. It does, however, necessitate a sum over all possible word sequences, which is intractable to compute in practice.…

Computation and Language · Computer Science 2022-04-22 Nils-Philipp Wynands , Wilfried Michel , Jan Rosendahl , Ralf Schlüter , Hermann Ney

Fine-tuning pre-trained models is a popular approach in machine learning for solving complex tasks with moderate data. However, fine-tuning the entire pre-trained model is ineffective in federated data scenarios where local data…

Machine Learning · Computer Science 2025-02-28 Pei-Yau Weng , Minh Hoang , Lam M. Nguyen , My T. Thai , Tsui-Wei Weng , Trong Nghia Hoang

Metaproteomics are becoming widely used in microbiome research for gaining insights into the functional state of the microbial community. Current metaproteomics studies are generally based on high-throughput tandem mass spectrometry (MS/MS)…

Quantitative Methods · Quantitative Biology 2020-09-24 Xuan Guo , Shichao Feng
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