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Capturing biological specimens at large scales with sub-micron resolution is crucial for biomedical research, but conventional cameras often can't handle the pixel requirements. While most microscopes use motorized stages to move samples…

Optics · Physics 2025-03-28 Charlie Kersuzan , Aymerick Bazin , Amaury Badon

Multifocus microscopy enables recording of entire volumes in a single camera exposure. In dense samples, multifocus microscopy is severely hampered by background haze. Here, we introduce a scalable multifocus method that incorporates…

Compound eyes found in insects provide intriguing sources of biological inspiration for miniaturized imaging systems. Inspired by such insect eye structures, we demonstrate an ultrathin arrayed camera enabled by a flat multilevel…

Hyperspectral imaging provides high-dimensional spatial-temporal-spectral information revealing intrinsic matter characteristics. Here we report an on-chip computational hyperspectral imaging framework with high spatial and temporal…

A novel THz near-field spectrometer is presented which allows to perform biological and medical studies with high spectral resolution combined with a spatial resolution down to l/100. In the setup an aperture much smaller than the used…

Biological Physics · Physics 2009-11-07 S. Mair , B. Gompf , M. Dressel

Microwave photonic radars enable fast or even real-time high-resolution imaging thanks to its broad bandwidth. Nevertheless, the frequency range of the radars usually overlaps with other existed radio-frequency (RF) applications, and only a…

Signal Processing · Electrical Eng. & Systems 2023-07-19 Cong Ma , Yue Yang , Ce Liu , Beichen Fan , Xingwei Ye , Yamei Zhang , Xiangchuan Wang , Shilong Pan

Total internal reflection fluorescence microscopy (TIRF) has enabled low-background, live-cell friendly imaging of cell surfaces and other thin samples thanks to the shallow penetration of the evanescent light field into the sample. The…

Hyperspectral microscopy is an imaging technique that provides spectroscopic information with high spatial resolution. When applied in the relevant wavelength region, such as in the infrared (IR), it can reveal a rich spectral fingerprint…

Optical super-resolution microscopy is a key technology for structural biology that offers high imaging contrast and live-cell compatibility. Minimal (fluorescence) photons flux microscopy, or MINFLUX, is an emerging super-resolution…

Optics · Physics 2025-08-29 Lukas Scheiderer , Zach Marin , Jonas Ries

Light sheet fluorescence microscopy provides optical sectioning and is widely used in volumetric imaging of large specimens. However, the axial resolution and the lateral Field of View (FoV) of the system, defined by the light sheet,…

Optics · Physics 2021-06-16 Hongjin Li , Zihan Wu , Zhichao Yang , Karl Zhanghao , Peng Xi , Dayong Jin

An image captured with a wide-aperture camera exhibits a finite depth-of-field, with focused and defocused pixels. A compact and robust representation of focus and defocus helps analyze and manipulate such images. In this work, we study the…

Computer Vision and Pattern Recognition · Computer Science 2020-12-22 Parikshit Sakurikar , P. J. Narayanan

We report on the recently emerging (Laser) Light Sheet based Fluorescence Microscopy field (LSFM). The techniques used in this field allow to study and visualize biomedical objects non-destructively in high-resolution through virtual…

Instrumentation and Detectors · Physics 2011-06-17 Jan A. N. Buytaert , Emilie Descamps , Dominique Adriaens , Joris J. J. Dirckx

To understand the scientific imaging capability, one must characterize the intra-pixel sensitivity variation (IPSV) of the CMOS image sensor. Extracting an IPSV map contributes to an improved detector calibration that allows to eliminate…

Instrumentation and Methods for Astrophysics · Physics 2018-01-11 Swaraj Mahato , J. De Ridder , Guy Meynants , Gert Raskin , H. Van Winckel

The mathematical theory of compressed sensing (CS) asserts that one can acquire signals from measurements whose rate is much lower than the total bandwidth. Whereas the CS theory is now well developed, challenges concerning hardware…

Information Theory · Computer Science 2013-07-18 Makhlad Chahid , Jerome Bobin , Hamed Shams Mousavi , Emmanuel Candes , Maxime Dahan , Vincent Studer

Microscopy is an essential tool in scientific research, enabling the visualization of structures at micro- and nanoscale resolutions. However, the field of microscopy often encounters limitations in field-of-view (FOV), restricting the…

Image and Video Processing · Electrical Eng. & Systems 2025-03-17 Huanhuan Zhao , Ruben Millan-Solsona , Marti Checa , Spenser R. Brown , Jennifer L. Morrell-Falvey , Liam Collins , Arpan Biswas

Optical spectrometers are widely used scientific equipment with many applications involving material characterization, chemical analysis, disease diagnostics, surveillance, etc. Emerging applications in biomedical and communication fields…

Wide-field mid-infrared photothermal (MIP) imaging offers rapid labelfree chemical contrast for biomedical and polymer analysis. However, its field of view (FOV) is limited by the pulse intensity of conventional infrared lasers. Here, we…

Two-Photon Microscopy has become an invaluable tool for biological and medical research, providing high sensitivity, molecular specificity, inherent three-dimensional sub-cellular resolution and deep tissue penetration. In terms of imaging…

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging sub-wavelength sized objects, leaving its mark in multiple branches of biology and technology. While several super-resolution optical microscopy…

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