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Far-field super-resolution fluorescence microscopy has been rapidly developed for applications ranging from cell biology to nanomaterials. However, it remains a significant challenge to achieve super-resolution imaging at depth in opaque…

Optics · Physics 2024-05-01 Tengfei Wu , YoonSeok Baek , Fei Xia , Sylvain Gigan , Hilton B. de Aguiar

Optical focusing through/inside scattering media, like multimode fiber and biological tissues, has significant impact in biomedicine yet considered challenging due to strong scattering nature of light. Previously, promising progress has…

On-invasive optical imaging techniques are essential diagnostic tools in many fields. Although various recent methods have been proposed to utilize and control light in multiple scattering media, non-invasive optical imaging through and…

Diffraction-limited imaging through complex scattering media is a long sought after goal with important applications in biomedical research. In recent years, high resolution wavefront-shaping has emerged as a powerful approach to generate a…

Optics · Physics 2014-10-17 Ori Katz , Eran Small , Yefeng Guan , Yaron Silberberg

Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution…

Biological Physics · Physics 2007-08-27 James H. Rice

Super-resolution fluorescence microscopy is an important tool in biomedical research for its ability to discern features smaller than the diffraction limit. However, due to its difficult implementation and high cost, the universal…

High-resolution optical microscopy has transformed biological imaging, yet its resolution and contrast deteriorate with depth due to multiple light scattering. Conventional correction strategies typically approximate the medium as one or a…

As light travels through a disordered medium such as biological tissues, it undergoes multiple scattering events. This phenomenon is detrimental to in-depth optical microscopy, as it causes a drastic degradation of contrast, resolution and…

The resolution of optical imaging devices is ultimately limited by the diffraction of light. To circumvent this limit, modern super-resolution microscopy techniques employ active interaction with the object by exploiting its optical…

Optics · Physics 2022-01-05 A. A. Pushkina , G. Maltese , J. I. Costa-Filho , P. Patel , A. I. Lvovsky

Photoacoustic tomography leverages ultrasound's deep tissue penetration to retrieve optical absorption contrast well beyond the optical diffusion limit. Conventional photoacoustic systems rely on externally delivered light and are therefore…

Optics · Physics 2026-01-06 David C. Garrett , Lihong V. Wang

Three-photon (3P) microscopy is getting traction due to its superior performance in deep tissues. Yet, aberrations and light scattering still pose one of the main limitations in the attainable depth ranges for high-resolution imaging. Here,…

We proposed a method to achieve superresolved optical imaging without beating the diffraction limit of light. This is achieved by magnifying the ideal optical image of the object through higher-order spatial frequency generation while…

Optics · Physics 2016-08-08 Zhixiang Li , Jianji Liu , Guoquan Zhang

Multiple light scattering hampers imaging objects in complex scattering media. Approaches used in real practices mainly aim to filter out multiple scattering obscuring the ballistic waves that travel straight through the scattering medium.…

The penetration depth of photoacoustic imaging in biological tissues has been fundamentally limited by the strong optical attenuation when light is delivered externally through the tissue surface. To address this issue, we previously…

Medical Physics · Physics 2021-02-24 Mucong Li , Tri Vu , Georgy Sankii , Brenton Winship , Kohldon Boydston , Russell Terry , Pei Zhong , Junjie Yao

Biomedical applications requiring tissue diagnosis, activation, and treatment could be substantially leveraged by optical methods, owing to their unique feature set. However, their widespread application is severely limited by the strong…

Optics · Physics 2019-10-23 Maxim Cherkashin , Carsten Brenner , Georg Schmitz , Martin Hofmann

Diffraction limit is manifested in the loss of high spatial frequency information that results from decay of evanescent waves. As a result, conventional far-field optics yields no information about an object's subwavelength features. Here…

Optics · Physics 2012-01-24 Leonid Alekseyev , Evgenii Narimanov , Jacob Khurgin

Light scattering by tissue severely limits how deep beneath the surface one can image, and the spatial resolution one can obtain from these images. Diffuse optical tomography (DOT) is one of the most powerful techniques for imaging deep…

Image and Video Processing · Electrical Eng. & Systems 2021-05-31 Yongyi Zhao , Ankit Raghuram , Hyun K. Kim , Andreas H. Hielscher , Jacob T. Robinson , Ashok Veeraraghavan

While scattered light conveys most of the information we perceive, scattering may also distort that information before it reaches our detectors. The problem is acute in many applications, such as in high-resolution microscopy of biological…

Optical imaging deep inside scattering media remains a fundamental problem in bio-imaging. While wavefront shaping has been shown to allow focusing of coherent light at depth, achieving it non-invasively remains a challenge. Various…

Optics · Physics 2019-12-24 Antoine Boniface , Baptiste Blochet , Jonathan Dong , Sylvain Gigan

We develop an imaging algorithm that exploits strong scattering to achieve super-resolution in changing random media. The method processes large and diverse array datasets using sparse dictionary learning, clustering, and multidimensional…