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We show the existence of a high interrelation between the different loops that may appear in a DNA segment. Conformational changes in a chain segment caused by the formation of a particular loop may either promote or prevent the appearance…

Biomolecules · Quantitative Biology 2009-03-24 Artur Garcia-Saez , J. Miguel Rubi

A class of nucleosome remodeling motors translocate nucleosomes, to which they are attached, toward the middle of DNA chain in the presence of ATP during in vitro experiments. Such a biological activity is likely based on a physical…

Biological Physics · Physics 2016-05-23 Kuni H. Iwasa , Ana Maria Florescu

Many fundamental biological processes are regulated by protein-DNA complexes called {\it synaptosomes}, which possess multiple interaction sites. Despite the critical importance of synaptosomes, the mechanisms of their formation remain not…

Soft Condensed Matter · Physics 2020-01-29 Cayke Felipe , Jaeoh Shin , Yulia Loginova , Anatoly B. Kolomeisky

Complex DNA topological structures, including polymer loops, are frequently observed in biological processes when protein molecules simultaneously bind to several distant sites on DNA. However, the molecular mechanisms of formation of these…

Soft Condensed Matter · Physics 2019-11-12 Jaeoh Shin , Anatoly B. Kolomeisky

The dynamics of a loop in DNA molecules at the denaturation transition is studied by scaling arguments and numerical simulations. The autocorrelation function of the state of complementary bases (either closed or open) is calculated. The…

Statistical Mechanics · Physics 2009-11-11 A. Bar , Y. Kafri , D. Mukamel

Cyclization of DNA with sticky ends is commonly used to construct DNA minicircles and to measure DNA bendability. The cyclization probability of short DNA (< 150 bp) has a strong length dependence, but how it depends on the rotational…

Biological Physics · Physics 2018-12-27 Jiyoun Jeong , Harold D. Kim

RNA sequencing (RNA-seq) enables characterization and quantification of individual transcriptomes as well as detection of patterns of allelic expression and alternative splicing. Current RNA-seq protocols depend on high-throughput…

Genomics · Quantitative Biology 2015-06-19 Hyunghoon Cho , Joe Davis , Xin Li , Kevin S. Smith , Alexis Battle , Stephen B. Montgomery

Genetic information is stored in a linear sequence of base-pairs; however, thermal fluctuations and complex DNA conformations such as folds and loops make it challenging to order genomic material for in vitro analysis. In this work, we…

Evolution of gene regulation is crucial for our understanding of the phenotypic differences between species, populations and individuals. Sequence-specific binding of transcription factors to the regulatory regions on the DNA is a key…

Populations and Evolution · Quantitative Biology 2015-11-09 Murat Tuğrul , Tiago Paixão , Nicholas H. Barton , Gašper Tkačik

We study the primary DNA structure of four of the most completely sequenced human chromosomes (including chromosome 19 which is the most dense in coding), using Non-extensive Statistics. We show that the exponents governing the decay of the…

Genomics · Quantitative Biology 2009-11-11 Th. Oikonomou , A. Provata

It has been reported that the number of transcription factors encoded in prokaryotic genomes scales approximately quadratically with their total number of genes. We propose a conceptual explanation of this finding and illustrate it using a…

Molecular Networks · Quantitative Biology 2010-09-24 Sergei Maslov , Sandeep Krishna , Tin Yau Pang , Kim Sneppen

The so called long range correlation properties of DNA sequences are studied using the variance analyses of the density distribution of a single or a group of nucleotides in a model independent way. This new method which was suggested…

Biological Physics · Physics 2007-05-23 A. K. Mohanty , A. V. S. S. Narayana Rao

We calculate the probability of DNA loop formation mediated by regulatory proteins such as Lac repressor (LacI), using a mathematical model of DNA elasticity. Our model is adapted to calculating quantities directly observable in Tethered…

Biomolecules · Quantitative Biology 2009-11-13 Kevin B. Towles , John F. Beausang , Hernan G. Garcia , Rob Phillips , Philip C. Nelson

Protein synthesis rates are determined, at the translational level, by properties of the transcript's sequence. The efficiency of an mRNA can be tuned by varying the ribosome binding sites controlling the recruitment of the ribosomes, or…

Subcellular Processes · Quantitative Biology 2017-12-14 Lucas D. Fernandes , Alessandro P. S. de Moura , Luca Ciandrini

DNA looping is important in DNA condensation and regulation. One method for forming a DNA loop, thought to be used by the condensing agent protamine, is bind-and-bend. In bind-and-bend, molecules bind all along the DNA, each creating a bend…

Biological Physics · Physics 2023-11-06 Michael Liaofan Liu , Daniel W. Oo , Ryan B. McMillan , Ashley R. Carter

Molecular biology explains function of molecules by their geometrical and electronical structures that are mainly determined by utilization of quantum effects in chemistry. However, further quantum effects are not thought to play any…

Quantum Physics · Physics 2011-12-30 Onur Pusuluk , Cemsinan Deliduman

DNA supercoiling plays an important role in a variety of cellular processes. The torsional stress related with supercoiling may be also involved in gene regulation through the local structure and dynamics of the double helix. To check this…

Biomolecules · Quantitative Biology 2015-05-19 Alexey K. Mazur

Topology affects physical and biological properties of DNA and impacts fundamental cellular processes, such as gene expression, genome replication, chromosome structure and segregation. In all organisms DNA topology is carefully modulated…

Biomolecules · Quantitative Biology 2019-03-27 P. Bettotti , V. Visone , L. Lunelli , G. Perugino , M. Ciaramella , A. Valenti

Tethered particle experiments use light microscopy to measure the position of a micrometer-sized bead tethered to a microscope slide via a ~micrometer length polymer, in order to infer the behavior of the invisible polymer. Currently, this…

Biological Physics · Physics 2009-11-13 John F. Beausang , Chiara Zurla , Carlo Manzo , David Dunlap , Laura Finzi , Philip C. Nelson

We study a minimal model for genome evolution whose elementary processes are single site mutation, duplication and deletion of sequence regions and insertion of random segments. These processes are found to generate long-range correlations…

Genomics · Quantitative Biology 2007-05-23 Philipp W. Messer , Peter F. Arndt , Michael Lässig