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The development of new quantum light sources requires robust and convenient methods of characterizing their joint spectral properties. Measuring the joint spectral intensity between a photon pair ignores any correlations in spectral phase…

Quantum Physics · Physics 2020-12-02 Bryn A. Bell , Gil Triginer Garces , Ian A. Walmsley

Fluctuation enhanced sensing (FES) is a promising method to improve the selectivity and sensitivity of semiconductor and nanotechnology gas sensors. Most measurement setups include high cost signal conditioning and data acquisition units as…

Instrumentation and Detectors · Physics 2014-05-07 Robert Mingesz , Gergely Vadai , Zoltan Gingl

The full understanding of cellular functions requires information about protein numbers for various biomolecular assemblies and their dynamics, which can be partly accessed by super-resolution fluorescence microscopy. Yet, many protein…

Biological Physics · Physics 2020-06-17 Kristin S. Grußmayer , Klaus Yserentant , Dirk-Peter Herten

The excited state population of single solid-state emitters is subjected to energy fluctuations around the equilibrium driven by the bath and relaxation through the emission of phonons or photons. Simultaneous measurement of the associated…

Quantum Physics · Physics 2021-05-12 Hendrik Utzat , Moungi G. Bawendi

Correlative microscopy is a powerful technique that combines the advantages of multiple imaging modalities to achieve a comprehensive understanding of investigated samples. For example, fluorescence microscopy provides unique functional…

State-of-the-art technology for pulse counter electronics offers an important range of commercial devices, but such systems are usually expensive due to the complex logic used for this task. The use of counting electronics in conjunction…

Instrumentation and Detectors · Physics 2017-06-16 E. Ipus , C. A. Melo-Luna , L. Giraldo , O. Vergara , J. H. Reina

The photon spectrum from free-electron laser (FEL) light sources offers valuable information in time-resolved experiments and machine optimization in the spectral and temporal domains. We have developed a compact single-shot photon…

Femtosecond transient soft X-ray Absorption Spectroscopy (XAS) is a very promising technique that can be employed at X-ray Free Electron Lasers (FELs) to investigate out-of-equilibrium dynamics for material and energy research. Here we…

"Single-shot" ultrafast spectroscopy based on the frequency encoding of transient absorbance kinetics using chirped probe pulses is analyzed theoretically. FDSS has an advantage over pump-probe spectroscopy in a situation where the "noise"…

Optics · Physics 2007-05-23 Ilya A. Shkrob , Stanislas Pommeret

We have developed a digital fast Fourier transform (FFT) spectrometer made of an analog-to-digital converter (ADC) and a field-programmable gate array (FPGA). The base instrument has independent ADC and FPGA modules, which allow us to…

Instrumentation and Methods for Astrophysics · Physics 2017-07-26 Kazumasa Iwai , Yûki Kubo , Hiromitsu Ishibashi , Takahiro Naoi , Kenichi Harada , Kenji Ema , Yoshinori Hayashi , Yuichi Chikahiro

Label-free optical microscopy through absorption or scattering spectroscopy provides fundamental insights across biology and materials science, yet its sensitivity remains fundamentally limited by photon shot noise. While recent…

A broad range of scientific and industrial disciplines require precise optical measurements at very low light levels. Single-photon detectors combining high efficiency and high time resolution are pivotal in such experiments. By using…

More than twenty years ago, scientists succeeded in pushing the limits of optical detection to single molecules using fluorescence. This breakthrough has revolutionized biophysical measurements, but restrictions in photophysics and labeling…

Biological Physics · Physics 2015-06-17 Marek Piliarik , Vahid Sandoghdar

Many cell signaling pathways involve the diffusion of messengers that bind/unbind to intracellular components. Quantifying their net transport rate under different conditions, then requires having separate estimates of their free diffusion…

Subcellular Processes · Quantitative Biology 2017-06-21 Lorena Sigaut , Cecilia Villarruel , María Laura Ponce , Silvina Ponce Dawson

Time-Correlated Single Photon Counting (TCSPC) and time tagging of individual photon detections are powerful tools in many quantum optical experiments and other areas of applied physics. Using TCSPC, e.g., for the purpose of fluorescence…

Instrumentation and Detectors · Physics 2020-01-09 Michael Wahl , Tino Roehlicke , Sebastian Kulisch , Sumeet Rohilla , Benedikt Kraeamer , Andreas C. Hocke

The ongoing integration of fine-grained power management features already established in CPU-driven Systems-on-Chip (SoCs) enables both traditional Field Programmable Gate Arrays (FPGAs) and, more recently, hybrid Programmable SoCs (pSoCs)…

Signal Processing · Electrical Eng. & Systems 2019-03-25 Martin Geier , Dominik Faller , Marian Brändle , Samarjit Chakraborty

The importance of spin-correlated radical pairs in biology is increasingly recognized, with roles in biological effects of weak magnetic fields and emerging quantum spin-based biomedical applications. Fluorescence microscopy offers…

Biological Physics · Physics 2025-10-08 Noboru Ikeya , Jonathan R. Woodward

Resonance energy transfer methods are in wide use for evaluating protein-protein interactions and protein conformational changes in living cells. Fluorescence resonance energy transfer (FRET) measures energy transfer as a function of the…

Biomolecules · Quantitative Biology 2011-05-05 Amparo Baíllo , Laura Martínez-Muñoz , Mario Mellado

Diffusion coefficient measurements are important for many biological and material investigations, such as particle dynamics, kinetics, and size determinations. Amongst current measurement methods, single particle tracking (SPT) offers the…

Biomolecules · Quantitative Biology 2011-07-12 Shannon Kian Zareh , Michael C. DeSantis , Jonathan Kessler , Je-Luen Li , Y. M. Wang