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In this study, we explore the potential of machine learning for modeling molecular electronic spectral intensities as a continuous function in a given wavelength range. Since presently available chemical space datasets provide excitation…

Chemical Physics · Physics 2022-08-02 Prakriti Kayastha , Sabyasachi Chakraborty , Raghunathan Ramakrishnan

A single photon source is a key enabling technology in device-independent quantum communication, quantum simulation for instance boson sampling, linear optics-based and measurement-based quantum computing. These applications involve many…

The study of binary pulsars enables tests of general relativity. Orbital motion in binary systems causes the apparent pulsar spin frequency to drift, reducing the sensitivity of periodicity searches. Acceleration searches are methods that…

Instrumentation and Methods for Astrophysics · Physics 2018-12-12 Sofia Dimoudi , Karel Adamek , Prabu Thiagaraj , Scott M. Ransom , Aris Karastergiou , Wesley Armour

Single-molecule fluorescence techniques provide exceptional sensitivity to probe biomolecular interactions. However, their application to accurately quantify analytes at the picomolar concentrations relevant for biosensing remains…

Optics · Physics 2025-02-18 Malavika Kayyil Veedu , Jérôme Wenger

Fluorescence recovery after photobleaching (FRAP) measurements offer an important tool for analyzing diffusion and binding processes. Confocal scanning laser microscopes that are used in FRAP experiments bleach regions with a radially…

Subcellular Processes · Quantitative Biology 2009-03-09 George D Tsibidis

Molecular fluorescence microscopy is a leading approach to super-resolution and nanoscale imaging in life and material sciences. However, super-resolution fluorescence microscopy is often bottlenecked by system-specific calibrations and…

The Penning-trap electronic-detection technique that offers the precision and sensitivity requested in mass spectrometry for fundamental studies in nuclear and particle physics has not been proven yet to be universal. This has motivated the…

Ultra-compact spectrometers are becoming increasingly popular for their promising applications in biomedical analysis, environmental monitoring, and food safety. In this work, we report a novel single-pixel-photodetector spectrometer with a…

Applied Physics · Physics 2023-06-08 Jingyi Wang , Beibei Pan , Zi Wang , Jiakai Zhang , Zhiqi Zhou , Lu Yao , Yanan Wu , Wuwei Ren , Jianyu Wang , Jingyi Yu , Baile Chen

Background-free fluorescence detection in multidimensional electronic spectroscopy promises high sensitivity compared to conventional approaches. Here we explore the sensitivity limits of multidimensional electronic spectroscopy. We present…

Chemical Physics · Physics 2022-10-18 Amitav Sahu , Vivek N. Bhat , Sanjoy Patra , Vivek Tiwari

Single photon sources (SPSs) based on quantum emitters hold promise in quantum radiometry as metrology standard for photon fluxes at the low light level. Ideally this requires control over the photon flux in a wide dynamic range,…

Quality control in molecular optical sectioning microscopy is indispensable for transforming acquired digital images from qualitative descriptions to quantitative data. Although numerous tools, metrics, and phantoms have been developed,…

Over the past 15 years there has been an ongoing debate regarding the influence of the photonic environment on Forster resonance energy transfer (FRET). Disparate results corresponding to enhancement, suppression and null effect of the…

Optics · Physics 2018-08-15 Cristian L. Cortes , Zubin Jacob

Fluorescent imaging plays a critical role in a myriad of scientific endeavors, particularly in the biological sciences. Three-dimensional imaging of fluorescent intensity often requires serial data acquisition, that is voxel-by-voxel…

Optics · Physics 2015-10-27 Jeffrey J. Field , David G. Winters , Randy A. Bartels

We report the cell biological applications of a recently developed multiphoton fluorescence lifetime imaging microscopy system using a streak camera (StreakFLIM). The system was calibrated with standard fluorophore specimens and was shown…

Cell Behavior · Quantitative Biology 2009-11-10 R. V. Krishnan , Eva Biener , Jian-Hua Zhang , Robert Heckel , Brian Herman

This document describes the technical design concept of a compact high intensity, multi-GeV photon source. Capable of producing 10^12 equivalent photons per second this novel device will provide unprecedented access to physics processes…

Instrumentation and Detectors · Physics 2020-03-18 D. Day , P. Degtiarenko , S. Dobbs , R. Ent , D. J. Hamilton , T. Horn , D. Keller , C. Keppel , G. Niculescu , P. Reid , I. Strakovsky , B. Wojtsekhowski , J. Zhang

Recent advances in Fluorescence Resonance Energy Transfer (FRET) provides a way to measure and understand different biological systems and molecular interactions in nanometer order. In this report the introduction and principle of the FRET…

General Physics · Physics 2009-08-14 Syed Arshad Hussain

Single Photon Emission Computed Tomography (SPECT) is a non-invasive imaging modality, frequently used in myocardial perfusion imaging. The biggest challenges facing the majority of clinical SPECT systems are low sensitivity, poor…

Medical Physics · Physics 2021-03-30 Narayan Bhusal

Stimulated Raman scattering (SRS) microscopy is a powerful label-free imaging technique that probes the vibrational response of chemicals with high specificity and sensitivity. High-power, quantum-enhanced SRS microscopes have been recently…

Frequency comb spectroscopy has significantly advanced molecular spectroscopy across scientific research and diverse applications. Among its key performance metrics especially for time-resolved studies, sensitivity and measurement speed are…

We present a single-photon-sensitive spectrometer, based on a linear array of 512 single-photon avalanche diode detectors, with 0.04 nm spectral and 40 ps temporal resolutions. We employ a fast data-driven operation that allows direct…

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