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Nanoscale secondary ion mass spectrometry (nanoSIMS) and fluorescence in situ hybridization (FISH) microscopy provide high-resolution, multimodal image representations of the identity and cell activity respectively of targeted microbial…

Computer Vision and Pattern Recognition · Computer Science 2022-04-07 Xiaojia He , Christof Meile , Suchendra M. Bhandarkar

Image partitioning, or segmentation without semantics, is the task of decomposing an image into distinct segments, or equivalently to detect closed contours. Most prior work either requires seeds, one per segment; or a threshold; or…

Computer Vision and Pattern Recognition · Computer Science 2021-04-20 Steffen Wolf , Alberto Bailoni , Constantin Pape , Nasim Rahaman , Anna Kreshuk , Ullrich Köthe , Fred A. Hamprecht

In medical imaging, chromosome straightening plays a significant role in the pathological study of chromosomes and in the development of cytogenetic maps. Whereas different approaches exist for the straightening task, typically geometric…

Computer Vision and Pattern Recognition · Computer Science 2021-10-20 Sifan Song , Daiyun Huang , Yalun Hu , Chunxiao Yang , Jia Meng , Fei Ma , Frans Coenen , Jiaming Zhang , Jionglong Su

Structured illumination microscopy (SIM) has attained high spatiotemporal delineation of subcellular architecture, yet offers limited insight into chemical composition. We develop Chem-SIM, a structured-illumination fluorescence detected…

We present a method for automatic cell classification in tissue samples using an automated training set from multiplexed immunofluorescence images. The method utilizes multiple markers stained in situ on a single tissue section on a robust…

Image and Video Processing · Electrical Eng. & Systems 2020-07-21 Alberto Santamaria-Pang , Anup Sood , Dan Meyer , Aritra Chowdhury , Fiona Ginty

Cryo-electron microscopy (cryo-EM) is a technique for reconstructing the 3-dimensional (3D) structure of biomolecules (especially large protein complexes and molecular assemblies). As the resolution increases to the near-atomic scale,…

Computer Vision and Pattern Recognition · Computer Science 2023-08-08 Weijie Chen , Xinyan Wang , Yuhang Wang

Understanding genetic variation, e.g., through mutations, in organisms is crucial to unravel their effects on the environment and human health. A fundamental characterization can be obtained by solving the haplotype assembly problem, which…

Genomics · Quantitative Biology 2022-10-25 Hansheng Xue , Vaibhav Rajan , Yu Lin

Phase microscopy is an invaluable tool in the biosciences and in clinical diagnostics. The sensitivity of current phase microscopy techniques is optimized for one specific mean phase value and varies significantly across a given sample.…

Optics · Physics 2019-08-30 Thomas Juffmann , Andrés de los Ríos Sommer , Sylvain Gigan

Multiplex immunofluorescence and immunohistochemistry benefit patients by allowing cancer pathologists to identify several proteins expressed on the surface of cells, enabling cell classification, better understanding of the tumour…

Computer Vision and Pattern Recognition · Computer Science 2021-07-19 Jessica Cooper , In Hwa Um , Ognjen Arandjelović , David J Harrison

Involved in mitotic condensation, interaction of transcriptional regulatory elements or isolation of structural domains, understanding loop formation is becoming a paradigm in the deciphering of chromatin architecture and its functional…

Quantitative Methods · Quantitative Biology 2024-09-17 Michaël Liefsoens , Timothy Földes , Maria Barbi

The rapid and accurate detection of biochemical compositions in fish is a crucial real-world task that facilitates optimal utilization and extraction of high-value products in the seafood industry. Raman spectroscopy provides a promising…

The expanding adoption of digital pathology has enabled the curation of large repositories of histology whole slide images (WSIs), which contain a wealth of information. Similar pathology image search offers the opportunity to comb through…

Computer Vision and Pattern Recognition · Computer Science 2021-07-30 Chengkuan Chen , Ming Y. Lu , Drew F. K. Williamson , Tiffany Y. Chen , Andrew J. Schaumberg , Faisal Mahmood

Histopathology image analysis plays a critical role in cancer diagnosis and treatment. To automatically segment the cancerous regions, fully supervised segmentation algorithms require labor-intensive and time-consuming labeling at the pixel…

Image and Video Processing · Electrical Eng. & Systems 2019-08-29 Gang Xu , Zhigang Song , Zhuo Sun , Calvin Ku , Zhe Yang , Cancheng Liu , Shuhao Wang , Jianpeng Ma , Wei Xu

Multi-echo Chemical Shift Encoded methods for Fat-Water quantification are growing in clinical use due to their ability to estimate and correct some confounding effects. State of the art CSE water-fat separation approaches rely on a…

Medical Physics · Physics 2017-06-15 Giulio Siracusano , Aurelio La Corte , Michele Gaeta , Giovanni Finocchio

Histo-pathological diagnostics are an inherent part of the everyday work but are particularly laborious and associated with time-consuming manual analysis of image data. In order to cope with the increasing diagnostic case numbers due to…

Morphological atlases are an important tool in organismal studies, and modern high-throughput Computed Tomography (CT) facilities can produce hundreds of full-body high-resolution volumetric images of organisms. However, creating an atlas…

Computer Vision and Pattern Recognition · Computer Science 2023-02-27 Jwalin Bhatt , Yaroslav Zharov , Sungho Suh , Tilo Baumbach , Vincent Heuveline , Paul Lukowicz

Many medical and biological protocols for analyzing individual biological cells involve morphological evaluation based on cell staining, designed to enhance imaging contrast and enable clinicians and biologists to differentiate between…

This paper presents an algorithm which aims to assist the radiologist in identifying breast cancer at its earlier stages. It combines several image processing techniques like image negative, thresholding and segmentation techniques for…

Computer Vision and Pattern Recognition · Computer Science 2009-11-04 Y. Ireaneus Anna Rejani , S. Thamarai Selvi

The segmentation, seen as the association of a partition with an image, is a difficult task. It can be decomposed in two steps: at first, a family of contours associated with a series of nested partitions (or hierarchy) is created and…

Computer Vision and Pattern Recognition · Computer Science 2016-09-12 Amin Fehri , Santiago Velasco-Forero , Fernand Meyer

Accurate segmentation of breast lesions is a crucial step in evaluating the characteristics of tumors. However, this is a challenging task, since breast lesions have sophisticated shape, topological structure, and variation in the intensity…

Computer Vision and Pattern Recognition · Computer Science 2018-02-26 Sulaiman Vesal , Nishant Ravikumar , Stephan Ellman , Andreas Maier