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Related papers: Differential Dynamic Microscopy: a High-Throughput…

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We demonstrate 'differential dynamic microscopy' (DDM) for the fast, high throughput characterization of the dynamics of active particles. Specifically, we characterize the swimming speed distribution and the fraction of motile cells in…

Quantitative Methods · Quantitative Biology 2011-07-12 Laurence G. Wilson , Vincent A. Martinez , Jana Schwarz-Linek , J. Tailleur , Peter N. Pusey , Gary Bryant , Wilson C. K. Poon

We characterize the full spatiotemporal gait of populations of swimming {\it Escherichia coli} using renewal processes to analyze the measurements of intermediate scattering functions. This allows us to demonstrate quantitatively how the…

We present the first time-resolved measurements of the oscillatory velocity field induced by swimming unicellular microorganisms. Confinement of the green alga C. reinhardtii in stabilized thin liquid films allows simultaneous tracking of…

Fluid Dynamics · Physics 2015-05-19 Jeffrey S. Guasto , Karl A. Johnson , J. P. Gollub

We use moving light patterns to control the motion of {\it Escherichia coli} bacteria whose motility is photo-activated. Varying the pattern speed controls the magnitude and direction of the bacterial flux, and therefore the accumulation of…

The experimental characterisation of the swimming statistics of populations of microorganisms or artificially propelled particles is essential for understanding the physics of active systems and their exploitation. Here, we construct a…

Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having…

Soft Condensed Matter · Physics 2014-11-07 Claudio Maggi , Alessia Lepore , Jacopo Solari , Alessandro Rizzo , Roberto Di Leonardo

One approach to quantifying biological diversity consists of characterizing the statistical distribution of specific properties of a taxonomic group or habitat. Microorganisms living in fluid environments, and for whom motility is key,…

Soft Condensed Matter · Physics 2020-12-03 Maciej Lisicki , Marcos F. Velho Rodrigues , Raymond E. Goldstein , Eric Lauga

The motility of microorganisms is influenced greatly by their hydrodynamic interactions with the fluidic environment they inhabit. We show by direct experimental observation of the bi-flagellated alga Chlamydomonas reinhardtii that fluid…

Biological Physics · Physics 2015-11-03 Boyang Qin , Arvind Gopinath , Jing Yang , Jerry P Gollub , Paulo E Arratia

The flagellated bacterium Escherichia coli is increasingly used experimentally as a self-propelled swimmer. To obtain meaningful, quantitative results that are comparable between different laboratories, reproducible protocols are needed to…

A swimming microorganism stirs the surrounding fluid, creating a flow field that governs not only its locomotion and nutrient uptake, but also its interactions with other microorganisms and the environment. Despite its fundamental…

Bacterial migration through confined spaces is critical for several phenomena like: biofilm formation, bacterial transport in soils, and bacterial therapy against cancer . In the present work, \textit{E. coli} (strain K12-MG1655 WT)…

Although the motility of the flagellated bacteria, Escherichia coli, has been widely studied, the effect of viscosity on swimming speed remains controversial. The swimming mode of wild-type E.coli is often idealized as a "run-and- tumble"…

Biological Physics · Physics 2018-05-09 Zijie Qu , Fatma Zeynep Temel , Rene Henderikx , Kenneth S. Breuer

In this fluid dynamics video, we present the first time-resolved measurements of the oscillatory velocity field induced by swimming unicellular microorganisms. Confinement of the green alga C. reinhardtii in stabilized thin liquid films…

Fluid Dynamics · Physics 2015-05-20 Jeffrey S. Guasto , Karl A. Johnson , J. P. Gollub

Understanding flow and transport of bacteria in porous media is crucial to technologies such as bioremediation, biomineralization or enhanced oil recovery. While physicochemical bacteria filtration is well-documented, recent studies showed…

Soft Condensed Matter · Physics 2022-08-24 Marco Dentz , Adama Creppy , Carine Douarche , Eric Clément , Harold Auradou

The microalga Chlamydomonas Reinhardtii (CR) is used here as a model system to study the effect of complex environments on the swimming of micro-organisms. Its motion can be modelled by a run and tumble mechanism so that it describes a…

Soft Condensed Matter · Physics 2019-03-27 Marvin Brun-Cosme-Bruny , Eric Bertin , Benoît Coasne , Philippe Peyla , Salima Rafaï

We show, using differential dynamic microscopy, that the diffusivity of non-motile cells in a three-dimensional (3D) population of motile E. coli is enhanced by an amount proportional to the active cell flux. While non-motile mutants…

Biological Physics · Physics 2013-11-07 A. Jepson , V. A. Martinez , J. Schwarz-Linek , A. Morozov , W. C. K. Poon

When treated with antibiotics below the minimum inhibitory concentration, bacterial cell division turns off, but cell growth does not. Thus, rod-like bacteria, including E. coli, can elongate many times their length without increasing their…

Soft Condensed Matter · Physics 2026-03-11 Richard Z. DeCurtis , Yongtae Ahn , Jane E. Hill , Sara M. Hashmi

Unraveling bacterial strategies for spatial exploration is crucial for understanding the complexity in the organization of life. Bacterial motility determines the spatio-temporal structure of microbial communities, controls infection…

In this fluid dynamics video, we demonstrate the microscale mixing enhancement of passive tracer particles in suspensions of swimming microalgae, Chlamydomonas reinhardtii. These biflagellated, single-celled eukaryotes (10 micron diameter)…

Swimming at a micrometer scale demands particular strategies. Indeed when inertia is negligible as compared to viscous forces (i.e. Reynolds number $Re$ is lower than unity), hydrodynamics equations are reversible in time. To achieve…

Soft Condensed Matter · Physics 2011-02-17 Michaël Garcia , Stefano Berti , Philippe Peyla , Salima Rafaï
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