English

Motility fractionation of bacteria by centrifugation

Soft Condensed Matter 2014-11-07 v1

Abstract

Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can be also used to separate swimming cells having different motility. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as "hot" colloidal particles having a diffusion coefficient that depends on the swimming speed.

Keywords

Cite

@article{arxiv.1310.2753,
  title  = {Motility fractionation of bacteria by centrifugation},
  author = {Claudio Maggi and Alessia Lepore and Jacopo Solari and Alessandro Rizzo and Roberto Di Leonardo},
  journal= {arXiv preprint arXiv:1310.2753},
  year   = {2014}
}

Comments

7 pages, 5 figures (in press)