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We previously developed an automatic track scanning system which enables the detection of large-angle nuclear fragments in the nuclear emulsion films of the OPERA experiment. As a next step, we have investigated this system's track…

Instrumentation and Detectors · Physics 2015-06-23 T. Fukuda , S. Fukunaga , H. Ishida , T. Matsumoto , T. Matsuo , S. Mikado , S. Nishimura , S. Ogawa , H. Shibuya , J. Sudou , A. Ariga , S. Tufanli

Microscopy imaging plays a vital role in understanding many biological processes in development and disease. The recent advances in automation of microscopes and development of methods and markers for live cell imaging has led to rapid…

Computer Vision and Pattern Recognition · Computer Science 2017-05-10 Saad Ullah Akram , Juho Kannala , Lauri Eklund , Janne Heikkilä

Single-molecule narrowfield microscopy is a versatile tool to investigate a diverse range of protein dynamics in live cells and has been extensively used in bacteria. Here, we describe how these methods can be extended to larger eukaryotic,…

Subcellular Processes · Quantitative Biology 2016-06-09 Adam J. M. Wollman , Mark C. Leake

We discuss a method for tracking individual molecules which globally optimizes the likelihood of the connections between molecule positions fast and with high reliability even for high spot densities and blinking molecules. Our method works…

Computer Vision and Pattern Recognition · Computer Science 2013-04-01 Andreas Karrenbauer , Dominik Wöll

Existing studies for automated melanoma diagnosis are based on single-time point images of lesions. However, melanocytic lesions de facto are progressively evolving and, moreover, benign lesions can progress into malignant melanoma.…

Computer Vision and Pattern Recognition · Computer Science 2020-06-22 Zhen Yu , Jennifer Nguyen , Xiaojun Chang , John Kelly , Catriona Mclean , Lei Zhang , Victoria Mar , Zongyuan Ge

The coarse-grained molecular dynamics (MD) or Brownian dynamics (BD) simulation is a particle-based approach that has been applied to a wide range of biological problems that involve interactions with surrounding fluid molecules or the…

Soft Condensed Matter · Physics 2014-11-20 Szu-Pei Fu , Yuan-Nan Young , Shidong Jiang

Tethered particle experiments use light microscopy to measure the position of a micrometer-sized bead tethered to a microscope slide via a ~micrometer length polymer, in order to infer the behavior of the invisible polymer. Currently, this…

Biological Physics · Physics 2009-11-13 John F. Beausang , Chiara Zurla , Carlo Manzo , David Dunlap , Laura Finzi , Philip C. Nelson

Optical detection of individual nanometer-sized analytes, virus particles, and protein molecules holds great promise for understanding and control of biological samples and healthcare applications. As fluorescent labels impose restrictions…

Optics · Physics 2020-10-19 Martin Dieter Baaske , Peter Sebastian Neu , Michel Orrit

Graphene serves critical application and research purposes in various fields. However, fabricating high-quality and large quantities of graphene is time-consuming and it requires heavy human resource labor costs. In this paper, we propose a…

Applied Physics · Physics 2021-03-26 Hui-Ying Siao , Siyu Qi , Zhi Ding , Chia-Yu Lin , Yu-Chiang Hsieh , Tse-Ming Chen

This paper presents a real-time approach for single-colored ball detection and tracking. The approach consists of two main phases. In a first offline calibration phase, the intrinsic parameters of the camera and the radial distortion are…

Computer Vision and Pattern Recognition · Computer Science 2014-10-16 Haythem Ghazouani

High-throughput screening using cell images is an efficient method for screening new candidates for pharmaceutical drugs. To complete the screening process, it is essential to have an efficient process for analyzing cell images. This paper…

Computer Vision and Pattern Recognition · Computer Science 2024-02-28 Mizuki Fukasawa , Tomokazu Fukuda , Takuya Akashi

We investigate the automatic differentiation of hybrid models, viz. models that may contain delays, logical tests and discontinuities or loops. We consider differentiation with respect to parameters, initial conditions or the time. We…

Systems and Control · Computer Science 2017-06-13 John Masse , Clara Masse , François Ollivier

The transport of macromolecules, such as DNA, through the cytoskeleton is critical to wide-ranging cellular processes from cytoplasmic streaming to transcription. The rigidity and steric hindrances imparted by the network of filaments…

Optical mapping by direct visualization of individual DNA molecules, stretched in nanochannels with sequence-specific fluorescent labeling, represents a promising tool for disease diagnostics and genomics. An important challenge for this…

Quantitative Methods · Quantitative Biology 2015-01-21 Charleston Noble , Adam N. Nilsson , Camilla Freitag , Jason P. Beech , Jonas O. Tegenfeldt , Tobias Ambjörnsson

Sequencing by tunneling is a next-generation approach to read single-base information using electronic tunneling transverse to the single-stranded DNA (ssDNA) backbone while the latter is translocated through a narrow channel. The original…

Soft Condensed Matter · Physics 2015-09-23 P. Boynton , A. V. Balatsky , I. K. Schuller , M. Di Ventra

Interstellar molecules, which play an important role in astrochemistry, are identified using observed spectral lines. Despite the advent of spectral analysis tools in the past decade, the identification of spectral lines remains a tedious…

Astrophysics of Galaxies · Physics 2025-02-07 Yisheng Qiu , Tianwei Zhang , Thomas Möller , XueJian Jiang , Zihao Song , Huaxi Chen , Donghui Quan

We present feature finding and tracking algorithms in 3D in living cells, and demonstrate their utility to measure metrics important in cell biological processes. We developed a computational imaging hybrid approach that combines automated…

Biological Physics · Physics 2015-04-06 Kemp Plumb , Sarah Elaz , Vincent Pelletier , Maria L. Kilfoil

Recent advances in scanning transmission electron and scanning tunneling microscopies allow researchers to measure materials structural and electronic properties, such as atomic displacements and charge density modulations, at an Angstrom…

A method enabling automatic detection and tracking of large amounts of individual dust particles in plasmas is presented. Individual trajectories can be found with a good spatiotemporal resolution, even without applying any external light…

Data Analysis, Statistics and Probability · Physics 2010-10-19 Y. Zayachuk , F. Brochard , S. Bardin , J-L. Briançon , R. Hugon , J. Bougdira

Tracking cells in 3D at high speed continues to attract extensive attention for many biomedical applications, such as monitoring immune cell migration and observing tumor metastasis in flowing blood vessels. Here, we propose a deep…

Optics · Physics 2018-05-16 Kan Liu , Hui Qiao , Jiamin Wu , Haoqian Wang , Lu Fang , Qionghai Dai