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We present a quantitative, multi-scale coarse-grained model of DNA coated colloids. The parameters of this model are transferable and are solely based on experimental data. As a test case, we focus on nano-sized colloids carrying…

Inspired by recent successes using single-stranded DNA tiles to produce complex structures, we develop a two-step coarse-graining approach that uses detailed thermodynamic calculations with oxDNA, a nucleotide-based model of DNA, to…

Soft Condensed Matter · Physics 2018-04-09 Pedro Fonseca , Flavio Romano , John S. Schreck , Thomas E. Ouldridge , Jonathan P. K. Doye , Ard A. Louis

To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that…

DNA is an astonishing material that can be used as a molecular building block to construct periodic arrays and devices with nanoscale accuracy and precision. Here, we present simple bead-spring model of DNA nanostars having three, four and…

Soft Condensed Matter · Physics 2021-10-22 Supriyo Naskar , Dhiraj Bhatia , Shiang-Tai Lin , Prabal K. Maiti

We introduce an extended version of oxDNA, a coarse-grained model of DNA designed to capture the thermodynamic, structural and mechanical properties of single- and double-stranded DNA. By including explicit major and minor grooves, and by…

We study the static and dynamical properties of DNA in the vicinity of its melting transition, i.e. the separation of the two strands upon heating. The investigation is based on a simple mechanical model which includes the helicoidal…

Statistical Mechanics · Physics 2007-06-19 Maria Barbi , Stefano Lepri , Michel Peyrard , Nikos Theodorakopoulos

We study theoretically the mechanical failure of a simple model of double stranded DNA under an applied shear. Starting from a more microscopic Hamiltonian that describes a sheared DNA, we arrive at a nonlinear generalization of a ladder…

Soft Condensed Matter · Physics 2009-04-13 Buddhapriya Chakrabarti , David R. Nelson

DNA bending is biologically important for genome regulation and is relevant to a range of nanotechnological systems. Recent results suggest that sharp bending is much easier than implied by the widely-used worm-like chain model; many of…

Biomolecules · Quantitative Biology 2015-07-01 Ryan M. Harrison , Flavio Romano , Thomas E. Ouldridge , Ard A. Louis , Jonathan P. K. Doye

Using theory and simulations, we carried out a first systematic characterization of DNA unzipping via nanopore translocation. Starting from partially unzipped states, we found three dynamical regimes depending on the applied force, f: (i)…

Soft Condensed Matter · Physics 2023-02-08 Antonio Suma , Vincenzo Carnevale , Cristian Micheletti

The simplest model of DNA mechanics describes the double helix as a continuous rod with twist and bend elasticity. Recent work has discussed the relevance of a little-studied coupling $G$ between twisting and bending, known to arise from…

Statistical Mechanics · Physics 2019-04-08 Stefanos K. Nomidis , Enrico Skoruppa , Enrico Carlon , John F. Marko

In this work we propose a model for DNA double helix within the tight-binding framework that incorporates the helicity of the molecules. We have studied localization properties of three DNAsequences,the periodic poly(dG)-poly(dC) and…

Mesoscale and Nanoscale Physics · Physics 2015-07-27 Sourav Kundu , S. N. Karmakar

Modeling of DNA-protein interactions is a complex process involving many important time and length scales. This can be facilitated through the use of coarse-grained models which reduce the number of degrees of freedom and allow efficient…

To study the elastic properties of rod-like DNA nanostructures, we perform long simulations of these structure using the oxDNA coarse-grained model. By analysing the fluctuations in these trajectories we obtain estimates of the bend and…

We apply a recently-developed coarse-grained model of DNA, designed to capture the basic physics of nanotechnological DNA systems, to the study of a `burnt-bridges' DNA motor consisting of a single-stranded cargo that steps processively…

Biological Physics · Physics 2014-11-14 Petr Šulc , Thomas E. Ouldridge , Flavio Romano , Jonathan P. K. Doye , Ard A. Louis

DNA hybridization is a fundamental reaction with wide-ranging applications in biotechnology. The nearest-neighbor (NN) model provides the most reliable description of the energetics of duplex formation. Most DNA thermodynamics studies have…

Biomolecules · Quantitative Biology 2024-04-30 Paolo Rissone , Marc Rico-Pasto , Steve Smith , Felix Ritort

Complementary DNA strands in solution reliably hybridize to form stable duplexes. We study the kinetics of the hybridization process and the mechanisms by which two initially isolated strands come together to form a stable double helix. We…

Biological Physics · Physics 2019-03-27 Raymond Jin , Lutz Maibaum

The computational modelling of DNA is becoming crucial in light of new advances in DNA nanotechnology, single-molecule experiments and in vivo DNA tampering. Here we present a mesoscopic model for double stranded DNA (dsDNA) at the single…

Soft Condensed Matter · Physics 2025-02-03 Y. A. G. Fosado , D. Michieletto , J. Allan , C. Brackley , O. Henrich , D. Marenduzzo

We studied the unzipping kinetics of single molecules of double-stranded DNA by pulling one of their two strands through a narrow protein pore. PCR analysis yielded the first direct proof of DNA unzipping in such a system. The time to unzip…

Soft Condensed Matter · Physics 2009-11-07 Alexis F. Sauer-Budge , Jacqueline A. Nyamwanda , David K. Lubensky , Daniel Branton

We present a phase-field model based on the Cahn-Hilliard equation to investigate the properties of phase separation in DNA nanostar systems. Leveraging a realistic free-energy functional derived from Wertheim theory, our model captures the…

Soft Condensed Matter · Physics 2025-04-23 Marco Cappa , Francesco Sciortino , Lorenzo Rovigatti

Short blunt-ended DNA duplexes comprising 6 to 20 base pairs self-assemble into polydisperse semi-flexible chains due to hydrophobic stacking interactions between terminal base pairs. Above a critical concentration, which depends on…

Soft Condensed Matter · Physics 2012-07-17 Cristiano De Michele , Lorenzo Rovigatti , Tommaso Bellini , Francesco Sciortino