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The lambda phage is a paradigm temperate bacteriophage. Its lysogenic and lytic life cycles echo competition between the DNA binding CI and CRO proteins. Here we address the Physics of this transition in terms of an energy function that…

Biomolecules · Quantitative Biology 2011-04-13 Andrei Krokhotine , Antti J. Niemi

The enterobacteria lambda phage is a paradigm temperate bacteriophage. Its lysogenic and lytic life cycles echo competition between the DNA binding $\lambda$-repressor (CI) and CRO proteins. Here we scrutinize the structure, stability and…

Biological Physics · Physics 2015-06-11 Andrey Krokhotin , Martin Lundgren , Antti J. Niemi

The two strands of a DNA molecule with a repetitive sequence can pair into many different basepairing patterns. For perfectly periodic sequences, early bulk experiments of Poerschke indicate the existence of a sliding process, permitting…

Biomolecules · Quantitative Biology 2007-05-23 Richard A. Neher , Ulrich Gerland

For cellular biochemical reaction systems where the numbers of molecules is small, significant noise is associated with chemical reaction events. This molecular noise can give rise to behavior that is very different from the predictions of…

Molecular Networks · Quantitative Biology 2009-11-13 Matthew Scott , Terence Hwa , Brian Ingalls

We propose a generic model of driven DNA under the influence of an oscillatory force of amplitude $F$ and frequency $\nu$ and show the existence of a dynamical transition for a chain of finite length. We find that the area of the hysteresis…

Soft Condensed Matter · Physics 2016-03-23 Sanjay Kumar , Ravinder Kumar , Wolfhard Janke

We calculate the probability of DNA loop formation mediated by regulatory proteins such as Lac repressor (LacI), using a mathematical model of DNA elasticity. Our model is adapted to calculating quantities directly observable in Tethered…

Biomolecules · Quantitative Biology 2009-11-13 Kevin B. Towles , John F. Beausang , Hernan G. Garcia , Rob Phillips , Philip C. Nelson

Delay is an inherent feature of genetic regulatory networks. It represents the time required for the assembly of functional regulator proteins. The protein production process is complex, as it includes transcription, translocation,…

Subcellular Processes · Quantitative Biology 2025-12-29 Sean Campbell , Courtney C. White , Amanda M. Alexander , William Ott

DNA methylation is usually treated as an epigenetic memory mark: transcriptional history is written into regulatory DNA and later stabilizes a chosen cell identity. This picture explains persistence, but it makes memory passive. Here we…

Molecular Networks · Quantitative Biology 2026-05-15 Kaifeng Wang , Ming Han

Active (catalysed) and passive (intrinsic) nucleosome repositioning is known to be a crucial event during the transcriptional activation of certain eucaryotic genes. Here we consider theoretically the intrinsic mechanism and study in detail…

Biological Physics · Physics 2009-11-07 I. M. Kulic , H. Schiessel

We use a statistical mechanical model to study nonthermal denaturation of DNA in the presence of protein-mediated loops. We find that looping proteins which randomly link DNA bases located at a distance along the chain could cause a…

Soft Condensed Matter · Physics 2009-12-22 K. G. Petrosyan , Chin-Kun Hu

In many cases, transcriptional regulation involves the binding of transcription factors at sites on the DNA that are not immediately adjacent to the promoter of interest. This action at a distance is often mediated by the formation of DNA…

Biomolecules · Quantitative Biology 2015-05-13 Lin Han , Hernan G. Garcia , Seth Blumberg , Kevin B. Towles , John F. Beausang , Philip C. Nelson , Rob Phillips

From a nanoscience perspective, cellular processes and their reduced in vitro imitations provide extraordinary examples for highly robust few or single molecule reaction pathways. A prime example are biochemical reactions involving DNA…

Biological Physics · Physics 2007-05-23 Ralf Metzler , Tobias Ambjoernsson , Andreas Hanke , Yongli Zhang , Stephen Levene

This paper presents an algorithm for approximating certain types of dynamical systems given by a system of ordinary delay differential equations by a Boolean network model. Often Boolean models are much simpler to understand than complex…

Molecular Networks · Quantitative Biology 2011-05-10 Franziska Hinkelmann , Reinhard Laubenbacher

During tissue development, patterns of gene expression determine the spatial arrangement of cell types. In many cases, gradients of secreted signaling molecules - morphogens - guide this process. The continuous positional information…

Molecular Networks · Quantitative Biology 2017-02-08 Ruben Perez-Carrasco , Pilar Guerrero , James Briscoe , Karen Page

It is well known that the structural deformations (stressed states) of DNA molecule play a crucial role in its biological functions including gene expression. For instance, looping in DNA (often mediated by protein binding) is a crucial…

Biological Physics · Physics 2007-05-23 Sachin Goyal , Noel C. Perkins

Cells use genetic switches to shift between alternate gene expression states, e.g., to adapt to new environments or to follow a developmental pathway. Here, we study the dynamics of switching in a generic-feedback on/off switch. Unlike…

Molecular Networks · Quantitative Biology 2015-05-27 Michael Assaf , Elijah Roberts , Zaida Luthey-Schulten

We develop a framework to discuss stability of epigenetic states as first exit problems in dynamical systems with noise. We consider in particular the stability of the lysogenic state of the lambda prophage, which is known to exhibit…

Soft Condensed Matter · Physics 2009-11-07 E. Aurell , K. Sneppen

DNA supercoiling plays an important role in a variety of cellular processes. The torsional stress related with supercoiling may be also involved in gene regulation through the local structure and dynamics of the double helix. To check this…

Biomolecules · Quantitative Biology 2015-05-19 Alexey K. Mazur

A versatile approach to modeling the conformations and energetics of DNA loops is presented. The model is based on the classical theory of elasticity, modified to describe the intrinsic twist and curvature of DNA, the DNA bending…

Biological Physics · Physics 2007-05-23 Alexander Balaeff , L. Mahadevan , Klaus Schulten

In many biochemical processes, proteins bound to DNA at distant sites are brought into close proximity by loops in the underlying DNA. For example, the function of some gene-regulatory proteins depends on such DNA looping interactions. We…

Quantitative Methods · Quantitative Biology 2009-11-13 John F Beausang , Philip C Nelson