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High-throughput 2D and 3D scanning electron microscopy, which relies on automation and dependable control algorithms, requires high image quality with minimal human intervention. Classical focus and astigmatism correction algorithms attempt…

Instrumentation and Detectors · Physics 2023-05-10 Philipp Johannes Schubert , Rangoli Saxena , Joergen Kornfeld

Photoacoustic imaging is the leading technique for deep tissue optical imaging, allowing single-shot imaging at depths. However, its resolution may be limited by acoustic aberrations, caused by natural unknown heterogeneities in the tissue…

Optics · Physics 2026-05-01 Yevgeny Slobodkin , Ori Katz

We propose an image resolution improvement method for optical coherence tomography (OCT) based on sparse continuous deconvolution. Traditional deconvolution techniques such as Lucy-Richardson deconvolution suffers from the artifact…

Biological Physics · Physics 2023-04-11 Zhengyu Qiao , Yong Huang , Qun Hao

Forward-viewing endoscopic optical coherence tomography (OCT) provides 3D imaging in vivo, and can be combined with widefield fluorescence imaging by use of a double-clad fiber. However, it is technically challenging to build a…

Allying high-resolution with a large field-of-view (FOV) is of great importance in the fields of biology and medicine, but particularly challenging when imaging non-flat living samples such as the human retina. Indeed, high-resolution is…

We present a method that takes as input a single dual-pixel image, and simultaneously estimates the image's defocus map -- the amount of defocus blur at each pixel -- and recovers an all-in-focus image. Our method is inspired from recent…

Computer Vision and Pattern Recognition · Computer Science 2021-10-13 Shumian Xin , Neal Wadhwa , Tianfan Xue , Jonathan T. Barron , Pratul P. Srinivasan , Jiawen Chen , Ioannis Gkioulekas , Rahul Garg

Full-field optical coherence tomography (FF-OCT) offers label-free, high-resolution imaging of biological samples but remains limited by slow acquisition due to piezoelectric mirror modulation. We present a continuous-scanning FF-OCT method…

Optics · Physics 2025-09-11 Charlie Kersuzan , Anirban Jana , Amaury Badon

The most realistic information about the transparent sample such as a live cell can be obtained only using bright-field light microscopy. At high-intensity pulsing LED illumination, we captured a primary 12-bit-per-channel (bpc) response…

Image and Video Processing · Electrical Eng. & Systems 2021-08-18 Ganna Platonova , Dalibor Stys , Pavel Soucek , Kirill Lonhus , Jan Valenta , Renata Rychtarikova

The purpose of the present work is a theoretical examination of how localized skin-tissue dehydration affects the depth of the confocal probing and what depth of effective detection can be reached with the chemical administration of skin…

Medical Physics · Physics 2007-05-23 I. V. Meglinski , D. Y. Churmakov , A. N. Bashkatov , E. A. Genina , V. V. Tuchin

This dissertation relates to the transition of the state of the art of swept source optical coherence tomography (SS-OCT) systems to a new realm in which the image acquisition speed is improved by an order of magnitude. With the aid of a…

Image and Video Processing · Electrical Eng. & Systems 2020-02-21 Amir Tofighi Zavareh

Spectral Domain Optical Coherence Tomography (SD-OCT) is a well-known imaging modality which allows for \textit{in-vivo} visualization of the morphology of different biological tissues at cellular level resolutions. The overall SD-OCT…

Traditional optical imaging faces an unavoidable trade-off between resolution and depth of field (DOF). To increase resolution, high numerical apertures (NA) are needed, but the associated large angular uncertainty results in a limited…

Currently, diagnosis of skin diseases is based primarily on visual pattern recognition skills and expertise of the physician observing the lesion. Even though dermatologists are trained to recognize patterns of morphology, it is still a…

A recent advance in optical coherence tomography (OCT), termed swept-source OCT, is generalized into a new technique, Fourier-domain OCT. It represents a realization of a full-field OCT system in place of the conventional serial image…

Dispersion is a common phenomenon in optics due to the frequency dependence of the refractive index in polychromatic light. This issue, if left untreated in optical coherence tomography (OCT) imaging, leads to signal broadening of the…

Image and Video Processing · Electrical Eng. & Systems 2022-01-03 Shangbang Luo , Guy Holland , Eric Mikula , Samantha Bradford , Reza Khazaeinezhad , James V Jester , Tibor Juhasz

Optical coherence tomography (OCT) has proven to be a powerful technique for studying tissue morphology in ophthalmology, cardiology, and endomicroscopy. Its performance is limited by the fundamental trade-off between the imaging…

Optics · Physics 2008-02-27 Keisuke Goda , Daniel R. Solli , Bahram Jalali

For measurements designed to accurately determine layer thickness, there is a natural trade-off between sensitivity to optical thickness and lateral resolution due to the angular ray distribution required for a focused beam. We demonstrate…

We have implemented a multi-functional optofluidic sensor that can monitor changes in the refractive index and pressure of biofluid simultaneously and can detect free-solution molecular interaction in-situ. In this paper, we demonstrate two…

Optics · Physics 2014-10-16 Zhida Xu , Logan Liu

In vivo application of dynamic optical coherence tomography (DOCT) is hindered by bulk motion of the sample. We demonstrate DOCT imaging of \invivo human skin by adopting a sample-fixation attachment to suppress bulk motion and a subsequent…

The performance of a confocal imaging system may be no better than a general-purpose widefield system if it is not properly maintained or quality controlled. The publication of ISO 21073, 'Confocal microscopes- Optical data of fluorescence…

Other Quantitative Biology · Quantitative Biology 2020-11-20 Glyn Nelson , Laurent Gelman , Orestis Faklaris , Roland Nitschke , Alex Laude