Related papers: How to Track Protists in Three Dimensions
We introduce a device developed to perform a 3D tracking of passive or active particles under flow, confined in a medium of hundreds micrometers wide. Micro-objects are placed inside a vertical glass capillary and two mirrors are set behind…
A three-dimensional (3D) scanning velocimetry system is developed to quantify the 3D configurations of particles and their surrounding volumetric, three-component velocity fields. The approach uses a translating laser sheet to rapidly scan…
Microscopes are essential for biomechanics and hydrodynamical investigation of small aquatic organisms. We report a DIY microscope (GLUBscope) that enables the visualization of organisms from two orthogonal imaging planes (top and side…
Fluid flow through intricate confining geometries often exhibits complex behaviors, certainly in porous materials, e.g. in groundwater flows or the operation of filtration devices and porous catalysts. However, it has remained extremely…
The collective behavior of levitated particles in a weakly-ionized plasma (dusty plasma) has raised significant scientific interest. This is due to the complex array of forces acting on the particles, and their potential to act as in-situ…
Object tracking is a key challenge of computer vision with various applications that all require different architectures. Most tracking systems have limitations such as constraining all movement to a 2D plane and they often track only one…
An innovative method based on the traversal of rays, originating from detected particles, through a three-dimensional grid of voxels is presented. The methodology has as main advantage that the outcome of the method is independent of the…
Three-dimensional particle tracking is an essential tool in studying dynamics under the microscope, namely, fluid dynamics in microfluidic devices, bacteria taxis, cellular trafficking. The 3d position can be determined using 2d imaging…
3D fluorescence microscopy of living organisms has increasingly become an essential and powerful tool in biomedical research and diagnosis. An exploding amount of imaging data has been collected, whereas efficient and effective…
How microorganisms respond to and interact with their environment can vary significantly from individual to individual, which can have important microbiological and ecological implications. However, most microscopy techniques can only…
Particle tracking velocimetry in 3D is becoming an increasingly important imaging tool in the study of fluid dynamics, combustion as well as plasmas. We introduce a dynamic discrete tomography algorithm for reconstructing particle…
Advancements in artificial active matter heavily rely on our ability to characterise their motion. Yet, the most widely used tool to analyse the latter is standard wide-field microscopy, which is largely limited to the study of…
We present recent advances in the instrumentation and analysis methods for quantitative imaging of concentrated colloidal suspensions under flow. After a brief review of colloidal imaging, we describe various flow geometries for two and and…
In this article, we consider a swimmer (i.e. a self-deformable body) immersed in a fluid, the flow of which is governed by the stationary Stokes equations. This model is relevant for studying the locomotion of microorganisms or micro robots…
We present a fast, high-throughput method for characterizing the motility of microorganisms in 3D based on standard imaging microscopy. Instead of tracking individual cells, we analyse the spatio-temporal fluctuations of the intensity in…
We study the distribution of swimming micro-organisms advected by a model turbulent flow and attracted towards a localised light source through phototaxis. It is shown that particles aggregate along a dynamical attractor with fractal…
We describe the development of a tracking device, mounted on an epi-fluorescent inverted microscope, suited to obtain time resolved 3D Lagrangian tracks of fluorescent passive or active micro-objects in micro-fluidic devices. The system is…
Persistent motion of microswimmers near boundaries is known to result in surface accumulation. Recently it was shown experimentally that surface accumulation of microswimmers is impacted primarily by steric forces and short-ranged…
A method for particle orientation tracking is developed and demonstrated specifically for anisotropic particles. Using (high-speed) multi-camera recordings of anisotropic particles from different viewpoints, we reconstruct the 3D location…
Tracking of plant cells in images obtained by microscope is a challenging problem due to biological phenomena such as large number of cells, non-uniform growth of different layers of the tightly packed plant cells and cell division.…