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We demonstrate with experiments and simulations how microscopic self-propelled particles navigate through environments presenting complex spatial features, which mimic the conditions inside cells, living organisms and future lab-on-a-chip…

Biological Physics · Physics 2011-09-22 Giovanni Volpe , Ivo Buttinoni , Dominik Vogt , Hans-Juergen Kuemmerer , Clemens Bechinger

An interferometric fluorescent microscope and a novel theoretic image reconstruction approach were developed and used to obtain super-resolution images of live biological samples and to enable dynamic real time tracking. The tracking…

Automated tracking of animal movement allows analyses that would not otherwise be possible by providing great quantities of data. The additional capability of tracking in realtime - with minimal latency - opens up the experimental…

Computer Vision and Pattern Recognition · Computer Science 2023-02-01 Andrew D. Straw , Kristin Branson , Titus R. Neumann , Michael H. Dickinson

The reconstruction of particle trajectories makes it possible to distinguish between different types of charged particles. In high-energy physics, where trajectories are rather long, large size trackers must be used to achieve sufficient…

Animals such as insects have provided a rich source of inspiration for designing robots. For example, animals navigate to goals via efficient coordination of individual motor actions, and demonstrate natural solutions to problems also faced…

Quantitative Methods · Quantitative Biology 2020-02-28 T. Thang Vo-Doan , Andrew D. Straw

Sorting, filtering, moving and controlling colloidal particles is crucial in many fields, ranging from chemistry to biology and physics. Dielectrophoresis is an outstanding tool for the manipulation of small particles by AC electric fields,…

Three-dimension real-time tracking of single emitters is an emerging tool for assessment of biological behavior as intraneuronal transport, for which spatiotemporal resolution is crucial to understand the microscopic interactions between…

Microscopic swimmers, e.g., chemotactic bacteria and cells, are capable of directed motion by exerting a force on their environment. For asymmetric microswimmers, e.g., bacteria, spermatozoa and many artificial active colloidal particles, a…

Soft Condensed Matter · Physics 2013-07-09 Mite Mijalkov , Giovanni Volpe

To study the behavior of freely moving model organisms such as zebrafish (Danio rerio) and fruit flies (Drosophila) across multiple spatial scales, it would be ideal to use a light microscope that can resolve 3D information over a wide…

A first-principle single-object model is proposed for pedestrian tracking. It is assumed that the extent of the moving object can be described via known statistics in 3D, such as pedestrian height. The proposed model thus need not constrain…

Computer Vision and Pattern Recognition · Computer Science 2024-12-12 Jan Krejčí , Oliver Kost , Ondřej Straka , Jindřich Duník

A particle tracking velocimetry apparatus is presented that is capable of measuring three-dimensional particle trajectories across large volumes, of the order of several meters, during natural snowfall events. Field experiments, aimed at…

Fluid Dynamics · Physics 2023-04-12 Nathaniel Bristow , Jiaqi Li , Peter Hartford , Michele Guala , Jiarong Hong

We characterize nano-textured surfaces by optical diffraction techniques using an adapted commercial light microscope with two detectors, a CCD camera and a spectrometer. The acquisition and analyzing time for the topological parameters…

In this article we present methods for measuring hindered Brownian motion in the confinement of complex 3D geometries using digital video microscopy. Here we discuss essential features of automated 3D particle tracking as well as diffusion…

Fluid Dynamics · Physics 2014-08-20 Simon L Dettmer , Ulrich F Keyser , Stefano Pagliara

X-ray microtomography is a versatile tool allowing the measurement of the 3D structure of optically thick samples. As a non-destructive technique, it is readily adapted to 4D imaging, where a sample can be monitored over time, and…

Instrumentation and Detectors · Physics 2025-12-17 Harry Allan , Tom Partridge , Joseph Jacob , Marco Endrizzi

Microscopic analysis of micro particles in situ in diverse water environments is necessary for monitoring water quality and localizing contamination sources. Conventional sensors such as optical microscopes and fluorometers often require…

Robotics · Computer Science 2019-11-26 Kevin Mallery , Dario Canelon , Jiarong Hong , Nikolaos Papanikolopoulos

Swimming cells and microorganisms must often move though complex fluids that contain an immersed microstructure such as polymer molecules, or filaments. In many important biological processes, such as mammalian reproduction and bacterial…

Fluid Dynamics · Physics 2018-08-06 Arshad Kamal , Eric E Keaveny

Microorganisms are rarely found in Nature swimming freely in an unbounded fluid. Instead, they typically encounter other organisms, hard walls, or deformable boundaries such as free interfaces or membranes. Hydrodynamic interactions between…

Fluid Dynamics · Physics 2013-10-21 Marcelo A. Dias , Thomas R. Powers

We present a new technique that combines off-axis Digital Holography and Dark Field Microscopy to track 100nm gold particles diffusing in water. We show that a single hologram is sufficient to localize several particles in a thick sample…

Optics · Physics 2015-06-03 Frédéric Verpillat , Fadwa Joud , Pierre Desbiolles , Michel Gross

3D cell tracking in a living organism has a crucial role in live cell image analysis. Cell tracking in C. elegans has two difficulties. First, cell migration in a consecutive frame is large since they move their head during scanning.…

Computer Vision and Pattern Recognition · Computer Science 2024-03-21 Kaito Shiku , Hiromitsu Shirai , Takeshi Ishihara , Ryoma Bise

We employ three numerical methods to explore the motion of low Reynolds number swimmers, modeling the hydrodynamic interactions by means of the Oseen tensor approximation, lattice Boltzmann simulations and multiparticle collision dynamics.…

Soft Condensed Matter · Physics 2007-05-23 David J. Earl , C. M. Pooley , J. F. Ryder , Irene Bredberg , J. M. Yeomans