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We carry out a theoretical study of the vibrational and relaxation properties of naturally-occurring proteins with the purpose of characterizing both the folding and equilibrium thermodynamics. By means of a suitable model we provide a full…

Statistical Mechanics · Physics 2007-05-23 Cristian Micheletti , Gianluca Lattanzi , Amos Maritan

Single-molecule experiments provide new insights into biological processes hitherto not accessible by measurements performed on bulk systems. We report on a study of the kinetics of a triple-branch DNA molecule with four conformational…

Biological Physics · Physics 2011-06-13 Sandra Engel , Anna Alemany , Nuria Forns , Philipp Maass , Felix Ritort

Acylphosphatase (AcP) is a small protein with 98 amino acid residues that catalyzes the hydrolysis of carboxyl-phosphate bonds. AcP is a typical two-state protein with slow folding rate due to its relatively large contact order in the…

Soft Condensed Matter · Physics 2022-03-14 Xuening Ma , Hao Sun , Haiyan Hong , Zilong Guo , Huanhuan Su , Hu Chen

Nanochannels provide means for detailed experiments on the effect of confinement on biomacromolecules, such as DNA. We here introduce a model for the complete unfolding of DNA from the circular to linear configuration. Two main ingredients…

I investigate a disordered version of a simplified model of protein folding, with binary degrees of freedom, applied to an ideal beta-hairpin structure. Disorder is introduced by assuming that the contact energies are independent and…

Disordered Systems and Neural Networks · Physics 2010-10-22 Marco Zamparo

The free energy of globular protein chain is considered to be a functional defined on smooth curves in three dimensional Euclidean space. From the requirement of geometrical invariance, together with basic facts on conformation of helical…

Statistical Mechanics · Physics 2007-05-23 V. V. Nesterenko , A. Feoli , G. Scarpetta

The understanding, and even the description of protein folding is impeded by the complexity of the process. Much of this complexity can be described and understood by taking a statistical approach to the energetics of protein conformation,…

chem-ph · Physics 2008-02-03 J. D. Bryngelson , J. N. Onuchic , N. D. Socci , P. G. Wolynes

Using the atomic force microscope based break junction approach, applicable to metal point contacts and single molecule junctions, measurements can be repeated thousands of times resulting in rich data sets characterizing the properties of…

Mesoscale and Nanoscale Physics · Physics 2017-03-08 Mark S. Hybertsen

Deviations from linearity in the dependence of the logarithm of protein unfolding rates, $\log k_u(f)$, as a function of mechanical force, $f$, measurable in single molecule experiments, can arise for many reasons. In particular, upward…

Soft Condensed Matter · Physics 2020-12-22 Pavel I. Zhuravlev , Michael Hinczewski , D. Thirumalai

A vast array of phenomena, ranging from chemical reactions to phase transformations, are analysed in terms of a free energy surface defined with respect to a single or multiple order parameters. Enhanced sampling methods are typically used,…

Statistical Mechanics · Physics 2023-04-26 Yagyik Goswami , Srikanth Sastry

We review the background, theory and general equations for the analysis of equilibrium protein unfolding experiments, focusing on denaturant and heat-induced unfolding. The primary focus is on the thermodynamics of reversible…

Biological Physics · Physics 2020-12-23 Kresten Lindorff-Larsen , Kaare Teilum

Brownian dynamics simulations are used to study the detachment of a particle from a substrate. Although the model is simple and generic, we attempt to map its energy, length and time scales onto a specific experimental system, namely a bead…

Soft Condensed Matter · Physics 2021-01-08 Emma Hodges , B. M. Cooke , E. M. Sevick , Debra J. Searles , B. Duenweg , J. Ravi Prakash

A geometric analysis of the global properties of the energy landscape of a minimalistic model of a polypeptide is presented, which is based on the relation between dynamical trajectories and geodesics of a suitable manifold, whose metric is…

Statistical Mechanics · Physics 2009-11-13 Lorenzo N. Mazzoni , Lapo Casetti

Normal mode analysis offers an efficient way of modeling the conformational flexibility of protein structures. Simple models defined by contact topology, known as elastic network models, have been used to model a variety of systems, but the…

Biomolecules · Quantitative Biology 2007-05-23 Dmitry A. Kondrashov , Adam W. Van Wynsberghe , Ryan M. Bannen , Qiang Cui , George N. Phillips

The assembly of proteins in membranes plays a key role in many crucial cellular pathways. Despite their importance, characterizing transmembrane assembly remains challenging for experiments and simulations. Equilibrium molecular dynamics…

Soft Condensed Matter · Physics 2024-08-05 Emil Jackel , Gianmarco Lazzeri , Roberto Covino

Mechanically induced unfolding of passive crosslinkers is a fundamental biological phenomenon encountered across the scales from individual macro-molecules to cytoskeletal actin networks. In this paper we study a conceptual model of…

Biological Physics · Physics 2015-01-08 M Caruel , J. -M Allain , L Truskinovsky

Protein folding and design are major biophysical problems, the solution of which would lead to important applications especially in medicine. Here a novel protein model capable of simultaneously provide quantitative protein design and…

Biological Physics · Physics 2015-06-22 Ivan Coluzza

Particles interacting with short-ranged potentials have attracted increasing interest, partly for their ability to model mesoscale systems such as colloids interacting via DNA or depletion. We consider the free energy landscape of such…

Mathematical Physics · Physics 2015-06-11 Miranda Holmes-Cerfon , Steven J. Gortler , Michael P. Brenner

Differential cross sections and analyzing powers for elastic scattering from, and for inelastic proton scattering to a set of $2^+_1$ states in, ${}^{12}$C, ${}^{20}$Ne, ${}^{24}$Mg, ${}^{28}$Si and ${}^{40}$Ca, and for a set of energies…

Nuclear Theory · Physics 2008-11-26 Y. J. Kim , K. Amos , S. Karataglidis , W. A. Richter

Single-molecule mechanical manipulation has enabled the quantitative understanding of the kinetics of bond ruptures as well as protein unfolding mechanism. Single-molecule experiments with theoretical models have allowed one to gain insight…

Biological Physics · Physics 2010-05-10 Gwonchan Yoon , Sungsoo Na , Kilho Eom