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Flow cytometry (FCM) is the standard multi-parameter assay for measuring single cell phenotype and functionality. It is commonly used for quantifying the relative frequencies of cell subsets in blood and disaggregated tissues. A typical…

Applications · Statistics 2020-09-01 Shai Gorsky , Cliburn Chan , Li Ma

We present a three-stage probabilistic model for the progression of Chronic Myeloid Leukemia (CML), as manifested by the leukemic stem cells, progenitor cells and mature leukemic cells. This progression is captured through the process of…

Populations and Evolution · Quantitative Biology 2022-04-25 Sonjoy Pan , Siddhartha P. Chakrabarty , Soumyendu Raha

In many reacting flow systems, the thermo-chemical state-space is known or assumed to evolve close to a low-dimensional manifold (LDM). Various approaches are available to obtain those manifolds and subsequently express the original…

Principal Component Analysis (PCA) is a classical method for reducing the dimensionality of data by projecting them onto a subspace that captures most of their variation. Effective use of PCA in modern applications requires understanding…

Statistics Theory · Mathematics 2019-06-14 David Hong , Laura Balzano , Jeffrey A. Fessler

Imaging mass spectrometry (IMS) has transformed proteomics by providing an avenue for collecting spatially distributed molecular data. Mass spectrometry data acquired with matrix assisted laser desorption ionization (MALDI) IMS consist of…

Applications · Statistics 2016-02-05 Lyron J. Winderbaum , Inge Koch , Ove J. R. Gustafsson , Stephan Meding , Peter Hoffmann

Powerful detectors at modern experimental facilities routinely collect data at multiple GB/s. Online analysis methods are needed to enable the collection of only interesting subsets of such massive data streams, such as by explicitly…

Distributed, Parallel, and Cluster Computing · Computer Science 2022-08-23 Rafael Vescovi , Ryan Chard , Nickolaus Saint , Ben Blaiszik , Jim Pruyne , Tekin Bicer , Alex Lavens , Zhengchun Liu , Michael E. Papka , Suresh Narayanan , Nicholas Schwarz , Kyle Chard , Ian Foster

Spatial Independent Components Analysis (ICA) is increasingly used in the context of functional Magnetic Resonance Imaging (fMRI) to study cognition and brain pathologies. Salient features present in some of the extracted Independent…

Significant advances in biotechnology have allowed for simultaneous measurement of molecular data points across multiple genomic and transcriptomic levels from a single tumor/cancer sample. This has motivated systematic approaches to…

In this paper we analyze approximate methods for undertaking a principal components analysis (PCA) on large data sets. PCA is a classical dimension reduction method that involves the projection of the data onto the subspace spanned by the…

Machine Learning · Statistics 2017-08-16 Darren Homrighausen , Daniel J. McDonald

Signals analysis for cytometry remains a challenging task that has a significant impact on uncertainty. Conventional cytometers assume that individual measurements are well characterized by simple properties such as the signal area, width,…

Acute Leukemia is the most common hematologic malignancy in children and adolescents. A key methodology in the diagnostic evaluation of this malignancy is immunophenotyping based on Multiparameter Flow Cytometry (FCM). However, this…

Machine Learning · Computer Science 2024-06-27 Elpiniki Maria Lygizou , Michael Reiter , Margarita Maurer-Granofszky , Michael Dworzak , Radu Grosu

Modern single-cell flow and mass cytometry technologies measure the expression of several proteins of the individual cells within a blood or tissue sample. Each profiled biological sample is thus represented by a set of hundreds of…

Machine Learning · Computer Science 2022-06-29 Siyuan Shan , Vishal Baskaran , Haidong Yi , Jolene Ranek , Natalie Stanley , Junier Oliva

Traditional principal component analysis (PCA) is well known in high-dimensional data analysis, but it requires to express data by a matrix with observations to be continuous. To overcome the limitations, a new method called flexible PCA…

Methodology · Statistics 2021-08-17 Tonglin Zhang , Baijian Yang , Qianqian Song , Jing Su

We present a reproducible deep learning pipeline for leukemic cell classification, focusing on system architecture, experimental robustness, and software design choices for medical image analysis. Acute lymphoblastic leukemia (ALL) is the…

Computer Vision and Pattern Recognition · Computer Science 2026-01-06 Douglas Costa Braga , Daniel Oliveira Dantas

How to extract useful insights from data is always a challenge, especially if the data is multidimensional. Often, the data can be organized according to certain hierarchical structure that are stemmed either from data collection process or…

Applications · Statistics 2016-04-21 Kun Yang , Wing Hung Wong

Perfusion analysis computes blood flow parameters (blood volume, blood flow, mean transit time) from the observed flow of contrast agent, passing through the patient's vascular system. Perfusion deconvolution has been widely accepted as the…

Computational Engineering, Finance, and Science · Computer Science 2010-06-02 Oleg Pianykh

The particle detectors at the future linear colliders, like ILD and SiD, use Particle Flow Algorithms (PFA)s to reach higher jet energy resolutions than the classical pure calorimetry. During the past few years, the University of Iowa group…

Instrumentation and Detectors · Physics 2012-03-02 Garabed Halladjian , Usha Mallik , Remi Zaidan

Sub-visible particle analysis using flow imaging microscopy combined with deep learning has proven effective in identifying particle types, enabling the distinction of harmless components such as silicone oil from protein particles.…

Computer Vision and Pattern Recognition · Computer Science 2025-08-11 Utku Ozbulak , Michaela Cohrs , Hristo L. Svilenov , Joris Vankerschaver , Wesley De Neve

High dimensional data has introduced challenges that are difficult to address when attempting to implement classical approaches of statistical process control. This has made it a topic of interest for research due in recent years. However,…

Applications · Statistics 2019-04-23 Mohammad Nabhan , Yajun Mei , Jianjun Shi

Intra-operative identification of malignant versus benign or healthy tissue is a major challenge in fluorescence guided cancer surgery. We propose a perfusion quantification method for computer-aided interpretation of subtle differences in…

Image and Video Processing · Electrical Eng. & Systems 2020-06-26 Sergiy Zhuk , Jonathan P. Epperlein , Rahul Nair , Seshu Thirupati , Pol Mac Aonghusa , Ronan Cahill , Donal O'Shea