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We present a study of the DNA translocation of the bacteriophage \phi 29 packaging molecular motor. From the experimental available information we present a model system based in an stochastic fashing potential, which reproduces the…

Biological Physics · Physics 2013-12-12 R. Perez-Carrasco , A. Fiasconaro , F. Falo , J. M. Sancho

Dynamics of the polymorphic A<->B transitions in DNA is compared for two polypurine sequences, poly(dA).poly(dT) and poly(dG).poly(dC), long known to exhibit contrasting properties in experiments. In free molecular dynamics simulations…

Biomolecules · Quantitative Biology 2007-05-23 Alexey K. Mazur

The understanding of mechanisms that control epigenetic changes is an important research area in modern functional biology. Epigenetic modifications such as DNA methylation are in general very stable over many cell divisions. DNA…

Genomics · Quantitative Biology 2019-01-21 Charalampos Kyriakopoulos , Pascal Giehr , Alexander Lück , Jörn Walter , Verena Wolf

Nanochannels provide means for detailed experiments on the effect of confinement on biomacromolecules, such as DNA. We here introduce a model for the complete unfolding of DNA from the circular to linear configuration. Two main ingredients…

Protonation of DNA basepairs is a reversible phenomenon which can be controlled by tuning the pH of the system. Under mild acidic conditions, the hydrogen bonding pattern of the DNA basepairs undergoes a change. We study the effect of…

Materials Science · Physics 2007-07-31 Sairam S. Mallajosyula , Swapan K Pati

Adaptation of the chemotaxis sensory pathway of the bacterium Escherichia coli is integral for detecting chemicals over a wide range of background concentrations, ultimately allowing cells to swim towards sources of attractant and away from…

Cell Behavior · Quantitative Biology 2015-05-18 Diana Clausznitzer , Olga Oleksiuk , Linda Lovdok , Victor Sourjik , Robert G. Endres

Escherichia coli bacteria respond to DNA damage by a highly orchestrated series of events known as the SOS response, regulated by transcription factors, protein-protein binding and active protein degradation. We present a dynamical model of…

Molecular Networks · Quantitative Biology 2007-05-23 Sandeep Krishna , Sergei Maslov , Kim Sneppen

Single molecule manipulation techniques reveal that the mechanical resistance of a protein depends on the direction of the applied force. Using a lattice model of polymers, we show that changing the pulling direction leads to different…

Statistical Mechanics · Physics 2009-11-13 Sanjay Kumar , Debaprasad Giri

Mutation is a critical mechanism by which evolution explores the functional landscape of proteins. Despite our ability to experimentally inflict mutations at will, it remains difficult to link sequence-level perturbations to systems-level…

Bacteriophages infect cells by attaching to the outer membrane and injecting their DNA into the cell.The phage DNA is then transcribed by the cell's transcription machinery.A number of physical mechanisms by which DNA can be translocated…

Biological Physics · Physics 2013-01-10 Sandip Ghosal

We explore the connection between a stochastic simulation model and an ordinary differential equations (ODEs) model of the dynamics of an excitable gene circuit that exhibits noise-induced oscillations. Near a bifurcation point in the ODE…

Molecular Networks · Quantitative Biology 2012-04-26 Robert C. Hilborn , Benjamin Brookshire , Jenna Mattingly , Anusha Purushotham , Anuraag Sharma

An ideal nanofabrication method should allow the organization of nanoparticles and molecules with nanometric positional precision, stoichiometric control and well-defined orientation. The DNA origami technique has evolved into a highly…

The percentage and sequence of AT and GC base pairs and charges on the DNA backbone contribute significantly to the stiffness of DNA. This elastic property of DNA also changes with small interacting ligands. The single-molecule force…

Soft Condensed Matter · Physics 2017-02-02 Anurag Singh , Amar Nath Gupta

Molecular-scale components are expected to be central to nanoscale electronic devices. While molecular-scale switching has been reported in atomic quantum point contacts, single-molecule junctions provide the additional flexibility of…

Fast, reliable and inexpensive DNA sequencing is an important pursuit in healthcare, especially in personalized medicine with possible deep societal impacts. Despite significant progress of various nanopore-based sequencing configurations,…

Gene expression in a cell entails random reaction events occurring over disparate time scales. Thus, molecular noise that often results in phenotypic and population-dynamic consequences sets a fundamental limit to biochemical signaling.…

Molecular Networks · Quantitative Biology 2009-02-19 C. -M. Ghim , E. Almaas

A general model for the early recognition and colocalization of homologous DNA sequences is proposed. We show, on a thermodynamic ground, how the distance between two homologous DNA sequences is spontaneously regulated by the concentration…

Subcellular Processes · Quantitative Biology 2008-09-30 M. Nicodemi , B. Panning , A. Prisco

For cellular biochemical reaction systems where the numbers of molecules is small, significant noise is associated with chemical reaction events. This molecular noise can give rise to behavior that is very different from the predictions of…

Molecular Networks · Quantitative Biology 2009-11-13 Matthew Scott , Terence Hwa , Brian Ingalls

Within systems biology there is an increasing interest in the stochastic behavior of genetic and biochemical reaction networks. An appropriate stochastic description is provided by the chemical master equation, which represents a continuous…

Biological Physics · Physics 2011-06-23 E. Giampieri , D. Remondini , L. de Oliveira , G. Castellani , P. Lió

The effect of sequence heterogeneity on polynucleotide translocation across a pore and on simple models of molecular motors such as helicases, DNA polymerase/exonuclease and RNA polymerase is studied in detail. Pore translocation of RNA or…

Statistical Mechanics · Physics 2009-11-10 Yariv Kafri , David K. Lubensky , David R. Nelson
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