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Multi-sample microarray experiments have become a standard experimental method for studying biological systems. A frequent goal in such studies is to unravel the regulatory relationships between genes. During the last few years, regression…

Applications · Statistics 2008-12-18 Nancy R. Zhang , Mary C. Wildermuth , Terence P. Speed

We consider the problem of detecting and estimating the strength of association between a trait of interest and alleles or haplotypes in a small genomic region (e.g. a gene or a gene complex), when no direct information on that region is…

Applications · Statistics 2008-04-11 Rodrigo Labouriau , Poul Sørensen , Helle R. Juul-Madsen

Gene expression and regulation rely on an apparently finely tuned set of reactions between some proteins and DNA. Such DNA-binding proteins have to find specific sequences on very long DNA molecules and they mostly do so in absence of any…

Biological Physics · Physics 2013-12-02 Maria Barbi , Fabien Paillusson

A central challenge in the study of protein evolution is the identification of historic amino acid sequence changes responsible for creating novel functions observed in present-day proteins. To address this problem, we developed a new…

Genomics · Quantitative Biology 2014-06-13 Victor Hanson-Smith , Christopher Baker , Alexander Johnson

Comprehensive knowledge of protein-ligand interactions should provide a useful basis for annotating protein functions, studying protein evolution, engineering enzymatic activity, and designing drugs. To investigate the diversity and…

Biomolecules · Quantitative Biology 2009-02-11 Akira R. Kinjo , Haruki Nakamura

We developed a method for estimating the positional distribution of transcription fac-tor (TF) binding sites using ChIP-chip data, and applied it to recently published experiments on binding sites of nine TFs; OCT4, SOX2, NANOG, HNF1A,…

Molecular Networks · Quantitative Biology 2008-11-11 Mark Koudritsky , Eytan Domany

Transcription factor concentrations provide signals to cells that allow them to regulate gene expression to make correct cell fate decisions. Calculations for noise bounds in gene regulation suggest that clustering or cooperative binding of…

Biological Physics · Physics 2026-01-09 Tamara Mijatović , Aimée R. Kok , Merlijn Brüggen , Jos W. Zwanikken , Marianne Bauer

The most common gene regulation mechanism is when a transcription factor protein binds to a regulatory sequence to increase or decrease RNA transcription. However, transcription factors face two main challenges when searching for these…

Quantitative Methods · Quantitative Biology 2023-11-21 Lucas Hedström , Ludvig Lizana

Transcription factors are proteins that regulate the expression of genes by binding to specific genomic regions known as Transcription Factor Binding Sites (TFBSs), typically located in the promoter regions of those genes. Accurate…

Machine Learning · Computer Science 2025-02-04 Nimisha Ghosh , Pratik Dutta , Daniele Santoni

Biological sequences may contain patterns that are signal important biomolecular functions; a classical example is regulation of gene expression by transcription factors that bind to specific patterns in genomic promoter regions. In motif…

Applications · Statistics 2015-06-04 Luis E. Carvalho

The transcription of DNA into mRNA is initiated and aided by a number of transcription factors (TFs), proteins with DNA-binding regions that attach themselves to binding sites in the DNA (transcription factor binding sites, TFBSs). As it…

Biomolecules · Quantitative Biology 2007-05-23 Mikael Huss , Karin Nordstrom

Next-generation sequencing technology enables the identification of thousands of gene regulatory sequences in many cell types and organisms. We consider the problem of testing if two such sequences differ in their number of binding site…

Genomics · Quantitative Biology 2014-02-04 Dennis Kostka , Tara Friedrich , Alisha K. Holloway , Katherine S. Pollard

Motivation: Alternative splicing is an important mechanism in which the regions of pre-mRNAs are differentially joined in order to form different transcript isoforms. Alternative splicing is involved in the regulation of normal…

Quantitative Methods · Quantitative Biology 2016-05-26 Hande Topa , Antti Honkela

Site-specific transcription factors (TFs) bind to their target sites on the DNA, where they regulate the rate at which genes are transcribed. Bacterial TFs undergo facilitated diffusion (a combination of 3D diffusion around and 1D random…

Molecular Networks · Quantitative Biology 2014-04-23 Daphne Ezer , Nicolae Radu Zabet , Boris Adryan

Statistical analysis of alignments of large numbers of protein sequences has revealed "sectors" of collectively coevolving amino acids in several protein families. Here, we show that selection acting on any functional property of a protein,…

Biomolecules · Quantitative Biology 2019-04-26 Shou-Wen Wang , Anne-Florence Bitbol , Ned S. Wingreen

DNA detection is of great significance in the point-of-care diagnostics. The stiffness of DNA, varying with its sequence and mechanochemical environment, could be a potential marker for DNA identification. The steric configurations of DNA…

Biological Physics · Physics 2019-08-06 Haiqian Yang , Liu Yang , Shaobao Liu

Transcription regulation is largely governed by the profile and the dynamics of transcription factors' binding to DNA. Stochastic effects are intrinsic to this dynamics and the binding to functional sites must be controled with a certain…

Genomics · Quantitative Biology 2007-05-23 Erik Aurell , Aymeric Fouquier d'Herouel , Claes Malmnas , Massimo Vergassola

Artificial synthesis of DNA molecules is an essential part of the study of biological mechanisms. The design of a synthetic DNA molecule usually involves many objectives. One of the important objectives is to eliminate short sequence…

Biomolecules · Quantitative Biology 2021-08-13 Zehavit Leibovich , Ilan Gronau

Biological systems have evolved to amazingly complex states, yet we do not understand in general how evolution operates to generate increasing genetic and functional complexity. Molecular recognition sites are short genome segments or…

Populations and Evolution · Quantitative Biology 2025-01-06 Tom Röschinger , Roberto Morán Tovar , Simone Pompei , Michael Lässig

A common problem in bioinformatics is related to identifying gene regulatory regions marked by relatively high frequencies of motifs, or deoxyribonucleic acid sequences that often code for transcription and enhancer proteins. Predicting…

Genomics · Quantitative Biology 2021-01-22 Ethan Jacob Moyer , Anup Das