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We develop revised theoretical ideas on the mechanism by which the transcription factor proteins locate their specific binding sites on DNA faster than the three-dimensional (3D) diffusion controlled rate limit. We demonstrate that the…

Biological Physics · Physics 2014-09-22 Rajamanickam Murugan

Evolution in its course found a variety of solutions to the same optimisation problem. The advent of high-throughput genomic sequencing has made available extensive data from which, in principle, one can infer the underlying structure on…

Quantitative Methods · Quantitative Biology 2016-04-12 Silvia Grigolon , Silvio Franz , Matteo Marsili

Several processes in the cell, such as gene regulation, start when key proteins recognise and bind to short DNA sequences. However, as these sequences can be hundreds of million times shorter than the genome, they are hard to find by simple…

Subcellular Processes · Quantitative Biology 2021-01-27 Markus Nyberg , Tobias Ambjörnsson , Per Stenberg , and Ludvig Lizana

We discuss a simple and powerful approach for the ab initio identification of cis-regulatory motifs involved in transcriptional regulation. The method we present integrates several elements: human-mouse comparison, statistical analysis of…

Genomics · Quantitative Biology 2007-05-23 Davide Cora' , Carl Herrmann , Christoph Dieterich , Ferdinando Di Cunto , Paolo Provero , Michele Caselle

The binding of a transcription factor (TF) to a DNA operator site can initiate or repress the expression of a gene. Computational prediction of sites recognized by a TF has traditionally relied upon knowledge of several cognate sites,…

Biomolecules · Quantitative Biology 2008-11-10 Sahand Jamal Rahi , Peter Virnau , Leonid A. Mirny , Mehran Kardar

Splice sites play a crucial role in gene expression, and accurate prediction of these sites in DNA sequences is essential for diagnosing and treating genetic disorders. We address the challenge of splice site prediction by introducing…

Genomics · Quantitative Biology 2023-11-23 Asmita Poddar , Vladimir Uzun , Elizabeth Tunbridge , Wilfried Haerty , Alejo Nevado-Holgado

Transcription factor proteins bind specific DNA sequences to control the expression of genes. They contain DNA binding domains which belong to several super-families, each with a specific mechanism of DNA binding. The total number of…

Biomolecules · Quantitative Biology 2010-07-27 Shalev Itzkovitz , Tsvi Tlusty , Uri Alon

We present new algorithms for the problem of multiple string matching of gapped patterns, where a gapped pattern is a sequence of strings such that there is a gap of fixed length between each two consecutive strings. The problem has…

Data Structures and Algorithms · Computer Science 2014-07-08 Emanuele Giaquinta , Kimmo Fredriksson , Szymon Grabowski , Alexandru I. Tomescu , Esko Ukkonen

The speed of site-specific binding of transcription factor (TFs) proteins with genomic DNA seems to be strongly retarded by the randomly occurring sequence traps. Traps are those DNA sequences sharing significant similarity with the…

Subcellular Processes · Quantitative Biology 2016-07-22 G. Niranjani , R. Murugan

Discovery of transcription factor binding sites is a much explored and still exploring area of research in functional genomics. Many computational tools have been developed for finding motifs and each of them has their own advantages as…

Computational Engineering, Finance, and Science · Computer Science 2011-07-07 K. R Seeja

Detection of false-positive motifs is one of the main causes of low performance in motif finding methods. It is generally assumed that false-positives are mostly due to algorithmic weakness of motif-finders. Here, however, we derive the…

Genomics · Quantitative Biology 2010-12-23 Amin Zia , Alan M. Moses

The discovery of motifs underlying gene expression is a challenging one. Some of these motifs are known transcription factors, but sequence inspection often provides valuable clues, even discovery of novel motifs with uncharacterized…

Genomics · Quantitative Biology 2007-05-23 Arvind Rao , Alfred O. Hero , David J. States , James Douglas Engel

When analyzing the genome, researchers have discovered that proteins bind to DNA based on certain patterns of the DNA sequence known as "motifs". However, it is difficult to manually construct motifs due to their complexity. Recently,…

Machine Learning · Computer Science 2017-02-23 Jack Lanchantin , Ritambhara Singh , Yanjun Qi

The problem of motif detection can be formulated as the construction of a discriminant function to separate sequences of a specific pattern from background. In computational biology, motif detection is used to predict DNA binding sites of a…

Genomics · Quantitative Biology 2010-12-10 Qing Zhou

A topological multiple testing approach to peak detection is proposed for the problem of detecting transcription factor binding sites in ChIP-Seq data. After kernel smoothing of the tag counts over the genome, the presence of a peak is…

Applications · Statistics 2013-05-29 Armin Schwartzman , Andrew Jaffe , Yulia Gavrilov , Clifford A. Meyer

The detection of similarities between long DNA and protein sequences is studied using concepts of statistical physics. It is shown that mutual similarities can be detected by sequence alignment methods only if their amount exceeds a…

Condensed Matter · Physics 2009-10-28 Terence Hwa , Michael Lassig

Response time and transcription level are vital parameters of gene regulation. They depend on how fast transcription factors (TFs) find and how efficient they occupy their specific target sites. It is well known that target site search is…

Biological Physics · Physics 2018-06-18 Johannes Hettich , J. Christof M. Gebhardt

One goal of human genetics is to understand how the information for precise and dynamic gene expression programs is encoded in the genome. The interactions of transcription factors (TFs) with DNA regulatory elements clearly play an…

Genomics · Quantitative Biology 2014-04-15 Darren A. Cusanovich , Bryan Pavlovic , Jonathan K. Pritchard , Yoav Gilad

Scoring DNA sequences against Position Weight Matrices (PWMs) is a widely adopted method to identify putative transcription factor binding sites. While common bioinformatics tools produce scores that can reflect the binding strength between…

Genomics · Quantitative Biology 2015-03-18 Xiaoyan Ma , Daphne Ezer , Carmen Navarro , Boris Adryan

Transcription factors (TFs) regulate gene expression through complex and co-operative mechanisms. While many TFs act together, the logic underlying TFs binding and their interactions is not fully understood yet. Most current approaches for…

Machine Learning · Computer Science 2026-03-13 Pietro Demurtas , Ferdinando Zanchetta , Giovanni Perini , Rita Fioresi