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Virus trafficking is fundamental for infection success and plasmid cytosolic trafficking is a key step of gene delivery. Based on the main physical properties of the cellular transport machinery such as microtubules, motor proteins, our…

Quantitative Methods · Quantitative Biology 2007-12-21 Thibault Lagache , David Holcman

Intracellular transport of DNA carriers is a fundamental step of gene delivery. We present here a theoretical approach to study generically a single virus or DNA particle trafficking in a cell cytoplasm. Cellular trafficking has been…

Quantitative Methods · Quantitative Biology 2007-11-28 T. Lagache , E. Dauty , D. Holcman

The nuclear pore supports molecular communication between cytoplasm and nucleus in eukaryotic cells. Selective transport of proteins is mediated by soluble receptors, whose regulation by the small GTPase Ran leads to cargo accumulation in,…

Subcellular Processes · Quantitative Biology 2009-03-17 R. B. Kopito , M. Elbaum

Nuclear pore complexes are constantly confronted by large fluxes of macromolecules and macromolecular complexes that need to get into and out of the nucleus. Such bi-directional traffic occurring in a narrow channel can easily lead to…

Biological Physics · Physics 2008-07-22 Ruti Kapon , Alon Topchik , David Mukamel , and Ziv Reich

The more we learn about the cytoplasm of cells, the more we realise that the cytoplasm is not uniform but instead is highly inhomogeneous. In any inhomogeneous solution, there are concentration gradients, and particles move either up or…

Subcellular Processes · Quantitative Biology 2019-04-03 Richard P. Sear

Internet, social media, neuronal or blood vessel are organized in complex networks. These networks are characterized by several quantities such as the underlying graph connectivity (topology), how they grow in time, scaling laws or by the…

Subcellular Processes · Quantitative Biology 2018-10-18 Matteo Dora , David Holcman

We propose an alternative mechanism for intracellular cargo transport which results from motor induced longitudinal fluctuations of cytoskeletal microtubules (MT). The longitudinal fluctuations combined with transient cargo binding to the…

Soft Condensed Matter · Physics 2007-05-23 Igor M. Kulić , Philip C. Nelson

Transport of molecular motors along protein filaments in a half-closed geometry is a common feature of biologically relevant processes in cellular protrusions. Using a lattice gas model we study how the interplay between active and…

Biological Physics · Physics 2017-03-30 Isabella R. Graf , Erwin Frey

Cells are strongly out-of-equilibrium systems driven by continuous energy supply. They carry out many vital functions requiring active transport of various ingredients and organelles, some being small, others being large. The cytoskeleton,…

Biological Physics · Physics 2017-09-20 Cecile Appert-Rolland , Maximilian Ebbinghaus , Ludger Santen

Intracellular transport is essential for maintaining proper cellular function in most eukaryotic cells, with perturbations in active transport resulting in several types of disease. Efficient delivery of critical cargos to specific…

Biological Physics · Physics 2016-08-16 David Ando , Nickolay Korabel , Kerwyn Casey Huang , Ajay Gopinathan

Intracellular components explore the cytoplasm via active motor-driven transport in conjunction with passive diffusion. We model the motion of organelles in narrow tubular cells using analytical techniques and numerical simulations to study…

Biological Physics · Physics 2018-04-11 Saurabh Mogre , Elena F. Koslover

Cells control the size and organization of biomolecular condensates formed by liquid-liquid phase separation (LLPS), but multiple mechanisms likely contribute to this control and remain to be fully elucidated. Here we propose a…

Biological Physics · Physics 2026-04-10 Le Qiao , Peter Gispert , Lukas S. Stelzl , Friederike Schmid

Molecular signalling in living cells occurs at low copy numbers and is thereby inherently limited by the noise imposed by thermal diffusion. The precision at which biochemical receptors can count signalling molecules is intimately related…

Statistical Mechanics · Physics 2016-09-26 Aljaz Godec , Ralf Metzler

Diffusion of tracer particles in the cytoplasm of mammalian cells is often anomalous with a marked heterogeneity even within individual particle trajectories. Despite considerable efforts, the mechanisms behind these observations have…

Biological Physics · Physics 2020-08-05 Adal Sabri , Xinran Xu , Diego Krapf , Matthias Weiss

Anomalously slow passive diffusion, $\langle \delta x^2(t)\rangle\simeq t^{\alpha}$, with $0<\alpha<1$, of larger tracers such as messenger RNA and endogenous submicron granules in the cytoplasm of living biological cells has been…

Biological Physics · Physics 2014-09-24 Igor Goychuk , Vasyl O. Kharchenko , Ralf Metzler

A certain class of viruses replicates inside a cell if they can enter the nucleus through one of many small target pores, before being permanently trapped or degraded. We adopt for viral motion a switching stochastic process model and we…

Subcellular Processes · Quantitative Biology 2016-07-20 Thibault Lagache , David Holcman

Intracellular transport is based on molecular motors that pull cargos along cytoskeletal filaments. One motor species always moves in one direction, e.g. conventional kinesin moves to the microtubule plus end, while cytoplasmic dynein moves…

Subcellular Processes · Quantitative Biology 2008-07-04 Melanie J. I. Müller , Stefan Klumpp , Reinhard Lipowsky

Living cells display a remarkable capacity to compartmentalize their functional biochemistry. A particularly fascinating example is the cell nucleus. Exchange of macromolecules between the nucleus and the surrounding cytoplasm does not…

Subcellular Processes · Quantitative Biology 2017-04-19 Ching-Hao Wang , Pankaj Mehta , Michael Elbaum

In this work we approach cell migration under a large-scale assumption, so that the system reduces to a particle in motion. Unlike classical particle models, the cell displacement results from its internal activity: the cell velocity is a…

Cell Behavior · Quantitative Biology 2018-08-02 Christèle Etchegaray , Nicolas Meunier

Cell movement, for example during embryogenesis or tumor metastasis, is a complex dynamical process resulting from an intricate interplay of multiple components of the cellular migration machinery. At first sight, the paths of migrating…

Cell Behavior · Quantitative Biology 2009-04-17 Peter Dieterich , Rainer Klages , Roland Preuss , Albrecht Schwab
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