English
Related papers

Related papers: Dynamic force spectroscopy on multiple bonds: expe…

200 papers

Analysis of bond rupture data from single-molecule force spectroscopy experiments commonly relies on the strong assumption that the bond dissociation process is irreversible. However, with increased spatiotemporal resolution of instruments…

Biological Physics · Physics 2022-05-13 Jakob Tómas Bullerjahn , Gerhard Hummer

Mechanical coupling between a cell and substrate relies on focal adhesions, clusters of adhesion proteins linking stress fibers (bundles of actin proteins) inside the cell with surrounding tissue. Focal adhesions have been demonstrated to…

Soft Condensed Matter · Physics 2007-05-23 Thomas Bickel , Robijn Bruinsma

In various cellular processes, biofilaments like F-actin and F-tubulin are able to exploit chemical energy associated to polymerization to perform mechanical work against an external load. The force-velocity relationship quantitatively…

Statistical Mechanics · Physics 2018-04-04 Alessia Perilli , Carlo Pierleoni , Giovanni Ciccotti , Jean-Paul Ryckaert

We extend the Bell forced dissociation rate model to take account into dynamic disorder. The motivation of the present work is from the recent forced dissociation experiments of the adhesive receptor-ligand complexes, in which some…

Soft Condensed Matter · Physics 2007-05-23 Fei Liu , Zhong-can Ou-Yang

In single-molecule force spectroscopy experiments, a biomolecule is attached to a force probe via polymer linkers, and the total extension -- of molecule plus apparatus -- is monitored as a function of time. In a typical unfolding…

Chemical Physics · Physics 2019-11-06 Roberto Covino , Michael T. Woodside , Gerhard Hummer , Attila Szabo , Pilar Cossio

Assessing the structural properties of large proteins is important to gain an understanding of their function in, e.g., biological systems or biomedical applications. We propose a method to examine the mechanical properties of proteins…

Single-molecule atomic force spectroscopy probes elastic properties of titin, ubiquitin and other relevant proteins. We explain bioprotein folding dynamics under both length- and force-clamp by modeling polyprotein modules as particles in a…

Statistical Mechanics · Physics 2016-11-17 L. L. Bonilla , A. Carpio , A. Prados

Using Langevin Dynamic simulations, we study effects of the shear force on the rupture of a double stranded DNA molecule. The model studied here contains two single diblock copolymers interacting with each other. The elastic constants of…

Soft Condensed Matter · Physics 2015-07-02 R. K. Mishra , T. Modi , D. Giri , S. Kumar

Mechanical forces acting on cell adhesion receptor proteins regulate a range of cellular functions by formation and rupture of non-covalent interactions with ligands. Typically, force decreases the lifetimes of intact complexes…

Biomolecules · Quantitative Biology 2014-06-16 Shaon Chakrabarti , Michael Hinczewski , D. Thirumalai

Time-resolved single-molecule biophysical experiments yield data that contain a wealth of dynamic information, in addition to the equilibrium distributions derived from histograms of the time series. In typical force spectroscopic setups…

Biomolecules · Quantitative Biology 2011-07-14 Michael Hinczewski , Yann von Hansen , Roland R. Netz

Single molecule force spectroscopy reveals unfolding of domains in titin upon stretching. We provide a theoretical framework for these experiments by computing the phase diagrams for force-induced unfolding of single domain proteins using…

Soft Condensed Matter · Physics 2009-10-31 D. K. Klimov , D. Thirumalai

The sliding friction force exerted by stochastic linkers interacting with a moving filament is calculated. The elastic properties of the substrate on which the linkers are anchored are shown to strongly influence the friction force. In some…

Soft Condensed Matter · Physics 2015-06-17 Pierre Sens

Single-molecule pulling experiments on unstructured proteins linked to neurodegenerative diseases have measured rupture forces comparable to those for stable folded proteins. To investigate the structural mechanisms of this unexpected force…

Biomolecules · Quantitative Biology 2013-06-19 S. Æ. Jónsson , S. Mitternacht , A. Irbäck

Mechanical stretching of secondary structures is studied through molecular dynamics simulations of a Go-like model. Force vs. displacement curves are studied as a function of the stiffness and velocity of the pulling device. The succession…

Soft Condensed Matter · Physics 2007-05-23 Marek Cieplak , Trinh Xuan Hoang , Mark O. Robbins

We solve the stochastic equations for a cluster of parallel bonds with shared constant loading, rebinding and the completely dissociated state as an absorbing boundary. In the small force regime, cluster lifetime grows only logarithmically…

Soft Condensed Matter · Physics 2007-05-23 T. Erdmann , U. S. Schwarz

A variety of experimental techniques have improved the 2D and 3D spatial resolution that can be extracted from \emph{in vivo} single-molecule measurements. This enables researchers to quantitatively infer the magnitude and directionality of…

Quantitative Methods · Quantitative Biology 2015-06-18 Christopher P. Calderon , Lucien E. Weiss , W. E. Moerner

We consider cigar shaped Fermi superfluid in the BEC-BCS crossover. Using polytropic form of equation of state, we derive low energy multibranch bosonic excitations and the corresponding density fluctuations in three different regimes along…

Other Condensed Matter · Physics 2007-09-08 Tarun Kanti Ghosh

Lifetimes of bound states of protein complexes or biomolecule folded states typically decrease when subject to mechanical force. However, a plethora of biological systems exhibit the counter-intuitive phenomenon of catch bonding, where…

Biomolecules · Quantitative Biology 2016-01-12 Shaon Chakrabarti , Michael Hinczewski , D. Thirumalai

We study the cooperative rupture of multiple adhesion bonds under shared linear loading. Simulations of the appropriate Master equation are compared with numerical integration of a rate equation for the mean number of bonds and its scaling…

Soft Condensed Matter · Physics 2007-05-23 T. Erdmann , U. S. Schwarz

We study the elastic properties of a single A/B copolymer chain with a specific sequence. We predict a rich structure in the force extension relations which can be addressed to the sequence. The variational method is introduced to probe…

Soft Condensed Matter · Physics 2009-11-07 Namkyung Lee , Thomas A. Vilgis