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Tracking single fluorescent molecules has offered resolution into dynamic molecular processes at the single-molecule level. This perspective traces the evolution of single-molecule tracking, highlighting key developments across various…

Biological Physics · Physics 2026-02-24 Lance W. Q. Xu , Steve Pressé

Despite significant advances in particle imaging technologies over the past two decades, few advances have been made in particle tracking, i.e. linking individual particle positions across time series data. The state-of-the-art tracking…

Soft Condensed Matter · Physics 2022-01-25 Ella M. King , Zizhao Wang , David A. Weitz , Frans Spaepen , Michael P. Brenner

We propose a new learning-based approach for 3D particle field imaging using holography. Our approach uses a U-net architecture incorporating residual connections, Swish activation, hologram preprocessing, and transfer learning to cope with…

Image and Video Processing · Electrical Eng. & Systems 2020-02-19 Siyao Shao , Kevin Mallery , Santosh Kumar , Jiarong Hong

Despite its potential for label-free particle diagnostics, holographic microscopy is limited by specialized processing methods that struggle to generalize across diverse settings. We introduce a deep learning architecture leveraging human…

Optics · Physics 2024-11-26 Shyam Kumar , Jiarong Hong

The adsorption of polymer and surfactant molecules onto colloidal particles or droplets in solution can be characterized non-destructively by scattering techniques. In a first part, the general framework of Dynamic Light Scattering, Small…

Soft Condensed Matter · Physics 2007-05-24 Julian Oberdisse

When a laser-cooled atomic sample is optically excited, the envelope of coherent forward scattering can often be decomposed into a few complex Gaussian profiles. The convenience of Gaussian propagation helps addressing key challenges in…

Atomic Physics · Physics 2024-12-31 Xing Huang , Yuzhuo Wang , Jian Zhao , Saijun Wu

We propose an autofocusing algorithm to obtain, relatively accurately, the 3D position of each particle, particularly its axial location, and particle number of a dense transparent particle solution via its hologram. First, morphological…

Signal Processing · Electrical Eng. & Systems 2025-03-25 Wei-Na Li , Yi Zhou , Jiatai Chen , Hongjie Ou , XiangSheng Xie

Digital holography (DH) has been widely used for imaging and characterization of micro and nanostructures in materials science and biology and has the potential to provide high-resolution, non-destructive measurements of fluid surfaces as…

Three-dimensional (3D) high-resolution imaging is essential in microscopy, yet light scattering poses significant challenges in achieving it. Here, we present an approach to holographic imaging of spatially incoherent objects through…

Optics · Physics 2025-02-04 YoonSeok Baek , Hilton B. de Aguiar , Sylvain Gigan

A method for particle orientation tracking is developed and demonstrated specifically for anisotropic particles. Using (high-speed) multi-camera recordings of anisotropic particles from different viewpoints, we reconstruct the 3D location…

Computer Vision and Pattern Recognition · Computer Science 2025-05-13 Mees M. Flapper , Elian Bernard , Sander G. Huisman

In the last fifteen years several techniques based on the holographic principle have been developed for the study of the 3D local order in solids. These methods use various particles: electrons, hard x-ray photons, gamma photons, or…

Condensed Matter · Physics 2009-11-10 G. Faigel , M Tegze , G. Bortel , Z. Jurek , S. Marchesini , M. Belakhovskyo , A. Simionovici

We characterize nano-textured surfaces by optical diffraction techniques using an adapted commercial light microscope with two detectors, a CCD camera and a spectrometer. The acquisition and analyzing time for the topological parameters…

By using scattering in near field techniques, a microscope can be easily turned into a device measuring static and dynamic light scattering, very useful for the characterization of nanoparticle dispersions. Up to now, microscopy based…

Optics · Physics 2015-05-13 D. Brogioli , D. Salerno , V. Cassina , F. Mantegazza

We propose a variant of the heterodyne holography scheme that combines the properties of off-axis and phase-shifting holography. This scheme makes it possible to filter off numerically the zero-order image alias and the technical noise of…

Optics · Physics 2009-11-13 Michel Gross , Michael Atlan

This paper describes an imaging microscopic technique based on heterodyne digital holography where subwavelength-sized gold colloids can be imaged in cell environment. Surface cellular receptors of 3T3 mouse fibroblasts are labeled with 40…

Since holography yields exact results, even in situations where perturbation theory is not applicable, it is an ideal framework for modeling strongly correlated systems. We extend previous holographic methods to take the dynamical charge…

High Energy Physics - Theory · Physics 2018-12-26 Ulf Gran , Marcus Tornsö , Tobias Zingg

Light scattering techniques are widely used in many fields of condensed and sof t matter physics. Usually these methods are based on the study of the scattered light in the far field. Recently, a new family of near field detection schemes…

Monitoring chemical reactions in solutions at the scale of individual entities is challenging: single particle detection requires small confocal volumes which are hardly compatible with Brownian motion, particularly when long integration…

Images of semiconductor `dot in rods' and their small clusters are studied by measuring the second-order correlation function with a spatially resolving ICCD camera. This measurement allows one to distinguish between a single dot and a…

Light scattering detection in the near field, a rapidly expanding family of scattering techniques, has recently proved to be an appropriate procedure for performing dynamic measurements. Here we report an innovative algorithm, based on the…

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